Intercellar reaction analysis using nanoparticles

• Project/Area Number: 18H02560
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
• Research Institution: Showa University
• Principal Investigator: Kato Masaru 昭和大学, 薬学部, 教授 (30332943)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2020: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2018: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: ナノ粒子 / タンパク質 / 精製 / 固相抽出 / HPLC / 細胞 / 細胞内局所 / 光 / 細胞外小胞 / 精製法 / 機能性物質 / 分離精製 / 光刺激 / 細胞内反応

Outline of Final Research Achievements

We have developed an excellent purification method for functional nanoparticles using cartridges or HPLC. In the HPLC method, discrimination was based on the surface potential of the nanoparticles and particles were separated based on the magnitude of the zeta potential. A technique using a solid-phase extraction cartridge enabled purification in a short period of time. By using these methods, it became possible to obtain uniform nanoparticles.

Academic Significance and Societal Importance of the Research Achievements

開発したナノ粒子の分離精製法は、簡便に均一なナノ粒子の入手が可能になった。また分析法は、ナノ粒子の表面電位に基づいて分離が行われるので、効率的な精製が可能になった。均一なナノ粒子は、内包物質の放出をより正確に制御することが可能であり、安全なナノ粒子の利用に貢献すると期待される。

Research Products

• [Journal Article] Quantitative determination of plasma cabozantinib concentration using HPLC-UV and its application to patients with renal cell carcinoma (2023)
  • Author(s): Maruyama Shinichi、Kato Masaru、Hiraga Tatsuru、Ishida Masaru、Kanno Hiroshi
  • Journal Title: Biomedical Chromatography Volume: 37 Issue: 5
  • DOI: 10.1002/bmc.5599
  • Peer Reviewed
• [Journal Article] Methylnigakinone content determination and geographical origin discrimination for P. quassioides via fluorescence fingerprint and principal component analyses (2022)
  • Author(s): Hamazaki Yasunori、Kato Masaru、Karasawa Koji
  • Journal Title: Journal of Pharmaceutical and Biomedical Analysis Volume: 219 Pages: 114932-114932
  • DOI: 10.1016/j.jpba.2022.114932
  • Peer Reviewed
• [Journal Article] A method for purifying nanoparticles using cationic modified monoliths and aqueous elution (2022)
  • Author(s): Kato Masaru、Yamaguchi Misa、Morita Tomoka、Watanabe Noriko、Ota Shigenori、Yamamoto Eiichi
  • Journal Title: Journal of Chromatography A Volume: 1664 Pages: 462802-462802
  • DOI: 10.1016/j.chroma.2021.462802
  • Peer Reviewed
• [Journal Article] Facile and simple purification method for small extracellular vesicles obtained from a culture medium through cationic particle capture (2021)
  • Author(s): Kato Masaru、Nakamoto Riho、Ishizuka Masaki、Watanabe Noriko
  • Journal Title: Analytical and Bioanalytical Chemistry Volume: 413 Issue: 9 Pages: 2523-2528
  • DOI: 10.1007/s00216-021-03207-9
  • Peer Reviewed
• [Journal Article] Stability and drug release studies of an antimycotic nanomedicine using HPLC, dynamic light scattering and atomic force microscopy (2018)
  • Author(s): A. Watanabe, M. Takagi, S. Murata, M. Kato,
  • Journal Title: Journal of Pharmaceutical Biomedical Analysis Volume: 148 Pages: 149-155
  • DOI: 10.1016/j.jpba.2017.09.030
  • Peer Reviewed
• [Journal Article] Enrichment of liposomal nanomedicines using monolithic solid phase extraction discs following preactivation with bivalent metal ion solutions (2018)
  • Author(s): Watanabe Ayako、Karasawa Koji、Murayama Shuhei、Sano Yoshihiro、Takagi Mio、Yamamoto Eiichi、Murata Shigeo、Kato Masaru
  • Journal Title: Journal of Chromatography A Volume: 1564 Pages: 224-227
  • DOI: 10.1016/j.chroma.2018.06.011
  • Peer Reviewed
• [Presentation] poly-Lys修飾HPLCカラムによる表面物性の異なる２種のナノ粒子の分離 (2023)
  • Author(s): 加藤 大、金井 優佳、萬田 夕貴、山本 栄一、 宮崎 将太、青山 千顕、秋武 将俊、太田 茂徳
  • Organizer: 日本薬学会第143年会
• [Presentation] HPLCによるナノメディシンの 評価法の開発 (2023)
  • Author(s): 加藤 大
  • Organizer: 第14回JBFシンポジウム
• [Presentation] 医療貢献を目指した分析化学 (2021)
  • Author(s): 加藤 大
  • Organizer: 第33回 バイオメディイカル分析科学シンポジウム
  • Invited
• [Presentation] HPLC column for 100nm nanoparticles analysis (2021)
  • Author(s): Masaru Kato
  • Organizer: PBA2021 Kyoto
  • Int'l Joint Research / Invited
• [Remarks] 昭和大学薬学部基礎薬学講座生体分析化学部門
  • URL: https://www.showa-u.ac.jp/education/pharm/major/bachem.html