Intercellar reaction analysis using nanoparticles
| Project/Area Number |
18H02560
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 47020:Pharmaceutical analytical chemistry and physicochemistry-related
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| Research Institution | Showa University |
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Principal Investigator |
Kato Masaru 昭和大学, 薬学部, 教授 (30332943)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2020: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2018: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
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| Keywords | ナノ粒子 / タンパク質 / 精製 / 固相抽出 / HPLC / 細胞 / 細胞内局所 / 光 / 細胞外小胞 / 精製法 / 機能性物質 / 分離精製 / 光刺激 / 細胞内反応 |
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| Outline of Final Research Achievements |
We have developed an excellent purification method for functional nanoparticles using cartridges or HPLC. In the HPLC method, discrimination was based on the surface potential of the nanoparticles and particles were separated based on the magnitude of the zeta potential. A technique using a solid-phase extraction cartridge enabled purification in a short period of time. By using these methods, it became possible to obtain uniform nanoparticles.
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| Academic Significance and Societal Importance of the Research Achievements |
開発したナノ粒子の分離精製法は、簡便に均一なナノ粒子の入手が可能になった。また分析法は、ナノ粒子の表面電位に基づいて分離が行われるので、効率的な精製が可能になった。均一なナノ粒子は、内包物質の放出をより正確に制御することが可能であり、安全なナノ粒子の利用に貢献すると期待される。
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Report
(5 results)
Research Products
(11 results)
