| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
In this study, we analyzed the behavior of endogenous exosomes and developed a method to attach immunoregulatory molecules to exosomes in order to develop in vivo gene immunotherapy using endogenous exosomes produced in the body. We focused on blood exosomes as endogenous exosomes, and succeeded in establishing a method to collect and label them. We selected NBD peptide, an NF-κB inhibitory peptide, as an immunoregulatory molecule, and succeeded in developing an efficient method for loading NBD peptide into exosomes. The NBD peptide-loaded exosomes showed a high anti-inflammatory effect. In conclusion, we have succeeded in establishing the basis for in vivo gene immunotherapy using endogenous exosomes.
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