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Analysis of ubiquitin network regulation by TRIM family proteins

Research Project

Project/Area Number 18H02607
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 48040:Medical biochemistry-related
Research InstitutionHokkaido University

Principal Investigator

Hatakeyama Shigetsugu  北海道大学, 医学研究院, 教授 (70294973)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywordsユビキチン / TRIMタンパク質 / 細胞内シグナル / 質量分析 / 細胞周期 / タンパク質分解 / 細胞内情報伝達
Outline of Final Research Achievements

It has been reported that TRIM family ubiquitin ligases are involved in the regulation of signal transduction and autophagy that control immune response, oncogenesis and cell differentiation, and has recently attracted attention in many fields of intracellular signal transduction system. In this application, we comprehensively analyzed the intracellular network system based on TRIM family ubiquitin ligase by comprehensive knockdown screening and proteomics method specialized in ubiquitination. Furthermore, by elucidating the functions of TRIM family in oncogenesis and the immune system, we proceeded to obtain findings that contribute to the diagnosis and treatment of autoimmune diseases, allergic diseases and cancers.

Academic Significance and Societal Importance of the Research Achievements

これまでは各TRIMタンパク質の機能が個別に報告されてきたが、本申請研究では網羅的にTRIMタンパク質が関するさまざまな細胞内シグナルを解析し、新たな基質タンパク質の同定に結び付いた。本研究成果により、TRIMファミリーによる翻訳後修飾(特にユビキチン化)の生理機能および疾患との関連の解明が進み、最終的にはがん化、細胞分化・個体発生・神経変性疾患・自己免疫疾患などの多様な方面おける総合的理解にも貢献する可能性が高い。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (12 results)

All 2020 2019 2018

All Journal Article (9 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 9 results,  Open Access: 4 results) Presentation (2 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] The role of Mediator and Little Elongation Complex in transcription termination2020

    • Author(s)
      Takahashi H, Ranjan A, Chen S, Suzuki H, Shibata M, Hirose T, Hirose H, Sasaki K, Abe R, Chen K, He Y, Zhang Y, Takigawa I, Tsukiyama T, Watanabe M, Fujii S, Iida M, Yamamoto J, Yamaguchi Y, Suzuki Y, Matsumoto M, Nakayama KI, Washburn P, Saraf A, Florens L, Sato S, Tomomori-Sato C, Conaway RC, Conaway JW, Hatakeyama S
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 1-20

    • DOI

      10.1038/s41467-020-14849-1

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] NONO is a negative regulator for SOX2 promoter.2020

    • Author(s)
      Liang S, Takahashi H, Hirose T, Kuramitsu Y, Hatakeyama S, Yoshiyama H, Wang R, Hamada J, Iizasa H.
    • Journal Title

      Cancer Genomics & Proteomics

      Volume: 17 Issue: 4 Pages: 359-367

    • DOI

      10.21873/cgp.20195

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis2020

    • Author(s)
      Sugisawa Erika、Takayama Yasunori、Takemura Naoki、Kondo Takeshi、Hatakeyama Shigetsugu、Kumagai Yutaro、Sunagawa Masataka、Tominaga Makoto、Maruyama Kenta
    • Journal Title

      Cell

      Volume: 182 Issue: 3 Pages: 609-624

    • DOI

      10.1016/j.cell.2020.06.022

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets2020

    • Author(s)
      Watanabe Masashi、Saeki Yasushi、Takahashi Hidehisa、Ohtake Fumiaki、Yoshida Yukiko、Kasuga Yusuke、Kondo Takeshi、Yaguchi Hiroaki、Suzuki Masanobu、Ishida Hiroki、Tanaka Keiji、Hatakeyama Shigetsugu
    • Journal Title

      Communications Biology

      Volume: 3 Issue: 1 Pages: 592-592

    • DOI

      10.1038/s42003-020-01328-y

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autophagy promotes citrullination of VIM (vimentin) and its interaction with major histocompatibility complex class II in synovial fibroblasts2019

    • Author(s)
      Sugawara Eri、Kato Masaru、Kudo Yuki、Lee Wenshi、Hisada Ryo、Fujieda Yuichiro、Oku Kenji、Bohgaki Toshiyuki、Amengual Olga、Yasuda Shinsuke、Onodera Tomohiro、Hatakeyama Shigetsugu、Atsumi Tatsuya
    • Journal Title

      Autophagy

      Volume: - Issue: 5 Pages: 1-10

    • DOI

      10.1080/15548627.2019.1664144

    • NAID

      120007032618

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Loss of TRIM29 Alters Keratin Distribution to Promote Cell Invasion in Squamous Cell Carcinoma.2018

    • Author(s)
      Yanagi T, Watanabe M, Hata H, Kitamura S, Imafuku K, Yanagi H, Homma A, Wang L, Takahashi H, Shimizu H, Hatakeyama S.
    • Journal Title

      Cancer Res

      Volume: 78 Issue: 24 Pages: 6795-6806

    • DOI

      10.1158/0008-5472.can-18-1495

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Brain-Derived Neurotrophic Factor Improves Limited Exercise Capacity in Mice With Heart Failure2018

    • Author(s)
      Matsumoto Junichi、et al. Hatakeyama Shigetsugu、Matsumoto Masaki、Nakayama Keiichi I.、Otsuka Yutaro、Sabe Hisataka、Tsutsui Hiroyuki、Anzai Toshihisa
    • Journal Title

      Circulation

      Volume: 138 Issue: 18 Pages: 2064-2066

    • DOI

      10.1161/circulationaha.118.035212

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Anti-Sez6l2 antibody, detected in a patient with immune-mediated cerebellar ataxia, inhibits complex formation of GluR1 and Sez6l22018

    • Author(s)
      Yaguchi, H., Yabe, I., Takahashi, H., Watanabe, M., Nomura, T., Kano, T., Watanabe, M. and Hatakeyama, S.
    • Journal Title

      J. Neurol.

      Volume: 265 Issue: 4 Pages: 962-965

    • DOI

      10.1007/s00415-018-8785-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TRIM59 promotes gliomagenesis by inhibiting TC45 dephosphorylation of STAT32018

    • Author(s)
      Sang, Y., Li, Y., Song, L., Alvarez, A.A., Zhang, W., Lv, D., Tang, J., Liu, F., Chang, Z., Hatakeyama, S., Hu, B., Cheng, S. and Feng, H.
    • Journal Title

      Cancer Res.

      Volume: 78 Issue: 7 Pages: 1792-1804

    • DOI

      10.1158/0008-5472.can-17-2774

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] TRIMファミリーの機能解析2019

    • Author(s)
      畠山鎮次
    • Organizer
      第2回ユビキチン研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Comprehensive identification of E3 ubiquitin ligase substrates by fusion of TUBE and ligase trapping methods2018

    • Author(s)
      Masashi watanabe, Shigetsugu Hatakeyama
    • Organizer
      The 2nd GI-CoRE GSQ, GSB & IGM Joint Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] 人体の構造と機能[2] 生化学 第14版2019

    • Author(s)
      畠山 鎮次
    • Total Pages
      304
    • Publisher
      医学書院
    • ISBN
      9784260035569
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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