| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Outline of Final Research Achievements |
Oxygen is essential for maintaining homeostasis of living organisms through various cell response mechanisms. However, the oxygen concentration to which individual cells are exposed in the living body is ambiguous. Here, by developing and improving the latest bioimaging technology using a multiphoton excitation microscope, we analyzed the oxygen concentration of individual cells in the tissues of living animals at the single cell level. We measured phosphorescence lifetime of osteoclasts in the bone marrow using the phosphorescence lifetime imaging method. As a result, we succeeded in determining the range of physiological oxygen concentration of osteoclasts is 2 to 5%.
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