| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
This study focuses on investigating novel epigenomic mechanisms which functionally link between nucleolus and mitochondria. Using specific siRNAs libraries, we found about 15 epigenetic factors that are involved in structure and function of nucleolus and mitochondria, and identified their target genes by RNA-seq and ChIP-seq. NSD2/WHSC1/MMSET methylase maintains growth-promoting gene activities to protect cellular senescence. In addition, we proposed the phenotypic variation in cellular senescence condition in fibroblasts. Further, LSD1/KDM1A and LSD2/KDM1B demethylases, nuclear enzymes that utilize the flavin adenosine dinucleotide as a cofactor, regulated energy metabolism in myocyte and adipocyte differentiation, respectively. Our findings indicate that these epigenetic factors have an essential role in controlling nucleolus and mitochondria metabolism.
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