Analysis of nucleolus-mitochondria network regulated by epigenetic factors
Project/Area Number |
18H02618
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 48040:Medical biochemistry-related
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Research Institution | Kumamoto University |
Principal Investigator |
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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Keywords | エピゲノム / 核小体 / ミトコンドリア / 遺伝子 / クロマチン / 代謝 |
Outline of Final Research Achievements |
This study focuses on investigating novel epigenomic mechanisms which functionally link between nucleolus and mitochondria. Using specific siRNAs libraries, we found about 15 epigenetic factors that are involved in structure and function of nucleolus and mitochondria, and identified their target genes by RNA-seq and ChIP-seq. NSD2/WHSC1/MMSET methylase maintains growth-promoting gene activities to protect cellular senescence. In addition, we proposed the phenotypic variation in cellular senescence condition in fibroblasts. Further, LSD1/KDM1A and LSD2/KDM1B demethylases, nuclear enzymes that utilize the flavin adenosine dinucleotide as a cofactor, regulated energy metabolism in myocyte and adipocyte differentiation, respectively. Our findings indicate that these epigenetic factors have an essential role in controlling nucleolus and mitochondria metabolism.
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Academic Significance and Societal Importance of the Research Achievements |
細胞の代謝の恒常性には、エネルギー(ATP)の合成と消費のバランスが重要である。その大半は、細胞質側のミトコンドリアでATPが合成され、核側の核小体によるリボソーム形成とタンパク質合成でATPが消費されている。このバランスを制御する機序については不明な点が多い。核小体とミトコンドリアの連動性に着目し、新規のエピゲノム制御機構を明らかにすることを目指した。核小体-ミトコンドリアのネットワークの基軸解明に迫り、生理と病態への関わりを明らかにするという学術的・社会的な意義がある。
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Report
(4 results)
Research Products
(62 results)
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[Journal Article] Expression of leukotriene B4 receptor 1 defines functionally distinct DCs that control allergic skin inflammation.2020
Author(s)
Tomoaki Koga, Fumiyuki Sasaki, Kazuko Saeki, Soken Tsuchiya, Toshiaki Okuno, Mai Ohba, Takako Ichiki, Satoshi Iwamoto, Hirotsugu Uzawa, Keiko Kitajima, Chikara Meno, Eri Nakamura, Norihiro Tada, Yoshinori Fukui, Junichi Kikuta, Masaru Ishii, Yukihiko Sugimoto, and Mitsuyoshi Nakao, Takehiko Yokomizo.
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Journal Title
Cellular & Molecular Immunology
Volume: 8
Issue: 6
Pages: 1-13
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Mesenchymal actomyosin contractility is required for androgen-driven urethral masculinization in mice.2019
Author(s)
Acebedo AR, Suzuki K, Hino S, Alcantara MC, Sato Y, Haga H, Matsumoto KI, Nakao M, Shimamura K, Takeo T, Nakagata N, Miyagawa S, Nishinakamura R, Adelstein RS, Yamada G.
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Journal Title
Commun. Biol.
Volume: eCollection
Issue: 1
Pages: 95-95
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] LSD1 mediates metabolic reprogramming by glucocorticoids during myogenic differentiation2018
Author(s)
K. Anan, S. Hino, N. Shimizu, A. Sakamoto, K. Nagaoka, R. Takase, K. Kohrogi, H. Araki, Y. Hino, S. Usuki, S. Oki, H. Tanaka, K. Nakamura, F. Endo, and M. Nakao
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Journal Title
Nucleic Acids Res.
Volume: 18
Issue: 11
Pages: 5441-5454
DOI
Related Report
Peer Reviewed / Open Access
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[Book] 生化学2019
Author(s)
阿南浩太郎、日野信次朗、中尾光善
Publisher
日本生化学会
Related Report
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