| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
RUNX is a transcription factor that plays an important role in hematopoietic cell differentiation. One of the regulatory mechanisms of gene expression via RUNX is the repression of target gene expression by RUNX-dependent gene silencers, but the regulatory mechanism is largely unknown.
In this study, we identified gene regions in close proximity to the silencer in Cd4 gene locus. As a result, genomic proximity between the silencer and enhancer regions was observed in situations where silencer activity is high, suggesting that silencer-induced suppression of gene expression is induced by targeting the enhancer. The regulation of enhancer RNA expression was also suggested as a mechanism of gene repression by silencer.
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