| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Outline of Final Research Achievements |
The thymic medulla is an anatomical region involved in establishing central self-tolerance. Hassall corpuscle (HC) is a characteristic keratinized structure within the thymic medulla; however, the role of HC on central tolerance is not well understood. In this study, we performed gene expression analysis of purified HC-constituting medullary thymic epithelial cells (mTEC). We revealed that HC-mTEC undergo cellular senescence in a steady state and express high levels of inflammatory molecules such as CXCL5 and IL-1 family members. Depletion of neutrophils, which express the CXCL5 receptor, suppresses the activation of pDCs in the thymic medulla. These results suggest that neutrophils are recruited and activated in response to CXCL5 produced as part of SASP from HC mTECs, and he activated neutrophils may induce pDCs activation in the thymic medulla. In summary, we succeeded in defining HC constitutive mTEC at the molecular level.
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