| Project/Area Number |
18H02654
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 49050:Bacteriology-related
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
松村 拓大 金沢大学, 医学系, 講師 (00456930)
油谷 雅広 金沢大学, 医学系, 助教 (20648810)
|
|---|
| Project Period (FY) |
2018-04-01 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2020)
|
| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
|
|---|
| Keywords | Clostridium botulinum / botulinum neurotoxin / hemagglutinin / ボツリヌス菌 / botulinum / 病原性 / ボツリヌス / Clostridium / 細菌毒素 / C. botulinum / クロストリジウム属 / Clostridia |
|---|
| Outline of Final Research Achievements |
C. botulinum produces botulinum neurotoxin complex which is composed of botulinum neurotoxin (BoNT) and non-toxic proteins, and causes botulism. Hemagglutinin component (HA) of non-toxic proteins is known to increase the oral toxicity of BoNT by enhancing the toxin absorption across the epithelial barrier of intestine. There exist ha genes negative strains, and all of these strains have orfx1~3, and p47 genes instead of ha genes. The biological function of these genes are unknown. In this study, we investigated the biological functions of these genes mainly using recombinant proteins of these gene products.
|
| Academic Significance and Societal Importance of the Research Achievements |
A2型ボツリヌス菌やE型ボツリヌス菌などHA遺伝子を持たない菌もHA遺伝子を持つ菌と同様にヒトに乳児ボツリヌス症や食中毒を起こす菌として知られており、これらはHA遺伝子の代わりにorfx1~3およびp47遺伝子を有する。そこでこれらの機能未知遺伝子がボツリヌス症の病態形成に関与している可能性について検討することは学術的に意義がある。
|
