Origin of drug resistant gene of Neisseria gonorrhoeae.
| Project/Area Number |
18H02659
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 49050:Bacteriology-related
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Ohnishi Makoto 国立感染症研究所, 副所長, 副所長 (10233214)
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| Co-Investigator(Kenkyū-buntansha) |
志牟田 健 国立感染症研究所, 細菌第一部, 主任研究官 (40370960)
中山 周一 国立感染症研究所, 細菌第一部, 主任研究官 (80280767)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2021: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Keywords | 淋菌 / 形質転換 / 薬剤耐性 / セフトリアキソン / 自然形質転換 |
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| Outline of Final Research Achievements |
To clarify the origin of drug resistance genes in Neisseria gonorrhoeae, we isolated 12 strains of ceftriaxone-resistant nonpathogenic Neisseria spp.(N. polysaccahrea, N. lactamica, N. cinerea and N. subflava). We revealed their genome sequence and characterized resistance genes. All of these ceftriaxone resistant genes were shown to be capable of transforming ceftriaxone susceptible Neisseria gonorrhoeae into resistant by. natural transformation using genome DNA of donors. Ceftriaxone resistant N. subflava, which could not directly transform N. gonorrhoeae into resistant , but could transform N. gonorrhoeae into resistant via other bacteria.
These results indicated that N. polysaccahrea, N. lactamica, N. cinerea and N. subflava formed a resistance gene pool harboring resistance genes of N. gonorrhoeae.
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| Academic Significance and Societal Importance of the Research Achievements |
淋菌の薬剤耐性化に非病原性ナイセリア属細菌が大きく影響を及ぼしている可能性を示した。淋菌を含むナイセリア属は遺伝子の共有化が可能であり、属としての共存進化の形の一端を示した意義は大きい。
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Report
(5 results)
Research Products
(19 results)
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[Journal Article] Clonal expansion and spread of the ceftriaxone-resistant Neisseria gonorrhoeae strain FC428, identified in Japan in 2015, and closely related isolates2019
Author(s)
203)Lee K, Nakayama SI, Osawa K, Yoshida H, Arakawa S, Furubayashi KI, Kameoka H, Shimuta K, Kawahata T, Unemo M, Ohnishi M.
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Journal Title
J Antimicrob Chemother
Volume: 74
Issue: 7
Pages: 1812-1819
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Multidrug-resistant Neisseria gonorrhoeae isolate, belonging to the internationally spreading Japanese FC428 clone, with ceftriaxone resistance and intermediate resistance to azithromycin, Ireland, August 2018.2018
Author(s)
Golparian D, Rose L, Lynam A, Mohamed A, Bercot B, Ohnishi M, Crowley B, Unemo M.
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Journal Title
Related Report
Peer Reviewed / Int'l Joint Research
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