| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2019: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
Rotaviruses are a major cause of severe gastroenteritis in infants and young children worldwide and are responsible for approximately 215,000 deaths annually, particularly in developing countries. The objective of this research is to understand how rotaviruses replicate in vitro and cause diseases in vivo using plasmid-based rotavirus reverse genetics systems. We established a plasmid-based reverse genetics system for G4P[8] human rotavirus strain Odelia. Using reverse genetics systems, we recovered a panel of monoreassortant viruses harboring one gene segment from strain Odelia within the simian rotavirus strain SA11 genetic backbone. We also tried to develop a reverse genetics system for murine rotavirus strain EW. Reassortant viruses between strains SA11 and EW generated by the rescue system enabled study of the biological functions of viral gene segments. These systems will provide insight into the molecular mechanisms underlying rotavirus replication and pathogenesis.
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