| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
The cell division of immune cells not only amplify antigen-specific clones but also determine the direction of differentiation, that is critical for determining how immunity work. Immune cells that respond to the antigen must be arrested at the appropriate time after the onset of division, but the regulatory mechanism is unclear. The Cdkn2a gene was identified by the combination of transcriptome and proteome for the molecular basis that controls the growth limit of lymphocytes. Among the proteins encoded by the Cdkn2a gene, we found that there was a correlation between the B cell proliferation limit and the Arf expression. In addition to Arf, we were able to identify new factors that epigenetically controling B cell proliferation limit. B cell-specific knockout mouse of candidate gene was generated, and analysis of the effects on germinal center B cell response duration and immune response has been performed.
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