Comprehensive investigation of tumorigenesis mechanism induced by sarcoma-related fusion genes

• Project/Area Number: 18H02677
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 50010:Tumor biology-related
• Research Institution: National Cancer Center Japan
• Principal Investigator: Kohsaka Shinji 国立研究開発法人国立がん研究センター, 研究所, ユニット長 (00627119)
• Co-Investigator(Kenkyū-buntansha): 椨 康一 東京医科歯科大学, 難治疾患研究所, 助教 (10466469)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 肉腫 / 融合遺伝子 / 機能解析 / バイオマテリアル

Outline of Final Research Achievements

Sarcoma-related fusion genes were integrated into 3T3, C2C12 and mouse mesenchymal stem cells and abilities of cell proliferation and differentiation inhibition were evaluated. Parental cells and oncogenic cells were labeled with individual fluorescent proteins and seeded on polymer slides to identify synthetic polymers on which oncogenic cells grow rapidly or attach strongly compared with parental cells. Established oncogenic cells were performed RNA-seq and conducted clustering analysis and Gene Set Enrichment Analysis to identify gene sets and signaling pathways specifically affected by respective fusion genes. Candidate molecular targeted drugs having specific inhibitory effects on fusion-introduced cells were screened and validated by individual drug sensitivity assay.

Academic Significance and Societal Importance of the Research Achievements

融合遺伝子特異的な遺伝子発現パターンが同定され, 融合遺伝子横断的な分類がすすみ, 融合遺伝子を標的とした分子標的薬の創薬に向けた分子プロファイリングの基盤が構築された. また融合遺伝子導入細胞特異的に増殖・接着する合成ポリマーが同定され, がん化や薬剤耐性などの細胞機能のスクリーニングに適したマテリアルの開発につながることが期待される.

Research Products

• [Journal Article] Rapid reprogramming of tumour cells into cancer stem cells on double-network hydrogels (2021)
  • Author(s): Suzuka J, Tsuda M, Wang L, Kohsaka S, Kishida K, Semba S, Sugino H, Aburatani S, Frauenlob M, Kurokawa T, Kojima S, Ueno T, Ohmiya Y, Mano H, Yasuda K, Gong JP, Tanaka S
  • Journal Title: Nat Biomed Eng Volume: － Issue: 8 Pages: 914-925
  • DOI: 10.1038/s41551-021-00692-2
  • Peer Reviewed / Open Access
• [Journal Article] The initial assessment of expert panel performance in core hospitals for cancer genomic medicine in Japan (2021)
  • Author(s): Sunami K, Naito Y, Aimono E, Amano T, Ennishi D, Kage H, Kanai M, Komine K, Koyama T, Maeda T, Morita S, Sakai D, Kohsaka S, Tsuchihara K, Yoshino T
  • Journal Title: Int J Clin Oncol Volume: 26 Issue: 3 Pages: 443-449
  • DOI: 10.1007/s10147-020-01844-1
  • Peer Reviewed / Open Access
• [Journal Article] Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single-cell level (2021)
  • Author(s): Takeda Nakamura I, Ikegami M, Hasegawa N, Hayashi T, Ueno T, Kawazu M, Yagishita S, Goto Y, Shinno Y, Kojima Y, Takamochi K, Takahashi F, Takahashi K, Mano H, Kohsaka S
  • Journal Title: Cancer Sci Volume: － Issue: 5 Pages: 2006-2019
  • DOI: 10.1111/cas.14821
  • Peer Reviewed / Open Access
• [Journal Article] Comprehensive molecular and clinicopathological profiling of desmoid tumours (2021)
  • Author(s): Kohsaka Shinji、Hirata Makoto、Ikegami Masachika、Ueno Toshihide、Kojima Shinya、Sakai Tomohisa、Ito Kan、Naka Norifumi、Ogura Koichi、Kawai Akira、Iwata Shintaro、Okuma Tomotake、Yonemoto Tsukasa、Kobayashi Hiroshi、Suehara Yoshiyuki、Hiraga Hiroaki、Kawamoto Teruya、Motoi Toru、Oda Yoshinao、Matsubara Daisuke、etc
  • Journal Title: European Journal of Cancer Volume: 145 Pages: 109-120
  • DOI: 10.1016/j.ejca.2020.12.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Extensive functional evaluation of exon 20 insertion mutations of EGFR (2021)
  • Author(s): Hirose T, Ikegami M, Endo M, Matsumoto Y, Nakashima Y, Mano H, and Kohsaka S
  • Journal Title: Lung Cancer Volume: 152 Pages: 135-142
  • DOI: 10.1016/j.lungcan.2020.12.023
  • Peer Reviewed / Open Access
• [Journal Article] Histological characteristics of lung adenocarcinoma with uncommon actionable alterations: special emphasis on MET exon 14 skipping alterations (2021)
  • Author(s): Hayashi Takuo、Kohsaka Shinji、Takamochi Kazuya、Kishikawa Satsuki、Ikarashi Daiki、Sano Kei、Hara Kieko、Onagi Hiroko、Suehara Yoshiyuki、Takahashi Fumiyuki、Saito Tsuyoshi、Nakatsura Tetsuya、Kitano Shigehisa、Suzuki Kenji、Yao Takashi
  • Journal Title: Histopathology Volume: - Issue: 7 Pages: 1-1
  • DOI: 10.1111/his.14311
  • Peer Reviewed / Open Access
• [Journal Article] RET inhibition in novel patient-derived models of RET-fusion positive lung adenocarcinoma reveals a role for MYC upregulation (2020)
  • Author(s): Hayashi T, Odintsov I, Smith RS, Ishizawa K, Liu AJW, Delasos L, Kurzatkowski C, Tai H, Gladstone E, Vojnic M, Kohsaka S, Suzawa K, Liu Z, Kunte S, Mattar MS, Khodos I, Davare MA, Drilon A, Cheng E, Stanchina E, Ladanyi M, Somwar R
  • Journal Title: Dis Model Mech Volume: － Issue: 2 Pages: 1-11
  • DOI: 10.1242/dmm.047779
  • Peer Reviewed / Open Access
• [Journal Article] Genetic Testing Distinguishes Multiple Chondroid Chordomas with Neuraxial Bone Metastases from Multicentric Tumors (2020)
  • Author(s): Kobayashi Hiroshi、Shin Masahiro、Makise Naohiro、Shinozaki-Ushiku Aya、Ikegami Masachika、Taniguchi Yuki、Shinoda Yusuke、Kohsaka Shinji、Ushiku Tetsuo、Oda Katsutoshi、Miyagawa Kiyoshi、Aburatani Hiroyuki、Mano Hiroyuki、Tanaka Sakae
  • Journal Title: Case Reports in Genetics Volume: 2020 Pages: 1-6
  • DOI: 10.1155/2020/8877722
  • Peer Reviewed / Open Access
• [Journal Article] Clinical practice guidance for next-generation sequencing in cancer diagnosis and treatment (edition 2) (2020)
  • Author(s): Naito Y, Aburatani H, Amano T, Baba E, Furukawa T, Hayashida T, Hiyama E, Ikeda S, Kanai M, Kato M, Kinoshita I, Kiyota N, Kohno T, Kohsaka S, et al.
  • Journal Title: Int J Clin Oncol Volume: － Issue: 2 Pages: 233-283
  • DOI: 10.1007/s10147-020-01831-6
  • Peer Reviewed / Open Access
• [Journal Article] Identification of a Novel MAN1A1-ROS1 Fusion Gene Through mRNA-based Screening for Tyrosine Kinase Gene Aberrations in a Patient with Leiomyosarcoma (2020)
  • Author(s): Suehara Y, Kohsaka S, Hayashi T, Akaike K, Kurisaki-Arakawa A, Sato S, Kobayashi E, Mizuno S, Ueno T, Morii T, Okuma T, Kurihara T, Hasegawa N, Sano K, Sasa K, Okubo T, Kim Y, Mano H, Saito T
  • Journal Title: Clin Orthop Relat Res Volume: － Issue: 4 Pages: 838-852
  • DOI: 10.1097/corr.0000000000001548
  • Peer Reviewed / Open Access
• [Journal Article] Nanostring-based screening for tyrosine kinase fusions in inflammatory myofibroblastic tumors (2020)
  • Author(s): Kurihara Taisei、Suehara Yoshiyuki、Akaike Keisuke、Hayashi Takuo、Kohsaka Shinji、Ueno Toshihide、Hasegawa Nobuhiko、Takagi Tatsuya、Sasa Keita、Okubo Taketo、Kim Youngji、Mano Hiroyuki、Yao Takashi、Kaneko Kazuo、Saito Tsuyoshi
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 1-1
  • DOI: 10.1038/s41598-020-75596-3
  • Peer Reviewed / Open Access
• [Journal Article] Diffuse expression of MUC6 defines a distinct clinicopathological subset of pulmonary invasive mucinous adenocarcinoma (2020)
  • Author(s): Kishikawa S, Hayashi T, Saito T, Takamochi K, Kohsaka S, Sano K, Sasahara N, Sasa K, Kurihara T, Hara K, Suehara Y, Takahashi F, Suzuki K, Yao T
  • Journal Title: Mod Pathol Volume: － Issue: 4 Pages: 786-797
  • DOI: 10.1038/s41379-020-00690-w
  • Peer Reviewed / Open Access
• [Journal Article] The Brain Microenvironment Induces DNMT1 Suppression and Indolence of Metastatic Cancer Cells (2020)
  • Author(s): Hirata Eishu、Ishibashi Kojiro、Kohsaka Shinji、Shinjo Keiko、Kojima Shinya、Kondo Yutaka、Mano Hiroyuki、Yano Seiji、Kiyokawa Etsuko、Sahai Erik
  • Journal Title: iScience Volume: 23 Issue: 9 Pages: 101480-101480
  • DOI: 10.1016/j.isci.2020.101480
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Clinicopathological characteristics of lung adenocarcinoma with compound EGFR mutations. (2020)
  • Author(s): Hayashi T, Kohsaka S, Takamochi K, Hara K, Kishikawa S, Sano K, Takahashi F, Suehara Y, Saito T, Takahashi K, Suzuki K, Yao T.
  • Journal Title: Human Pathology Volume: 14 Pages: 42-51
  • DOI: 10.1016/j.humpath.2020.07.007
  • Peer Reviewed
• [Journal Article] Comprehensive clinicopathological and molecular analysis of primary malignant melanoma of the oesophagus (2020)
  • Author(s): Tsuyama Sho、Kohsaka Shinji、Hayashi Takuo、Suehara Yoshiyuki、Hashimoto Takashi、Kajiyama Yoshiaki、Tsurumaru Masahiko、Ueno Toshihide、Mano Hiroyuki、Yao Takashi、Saito Tsuyoshi
  • Journal Title: Histopathology Volume: 78 Issue: 2 Pages: 240-251
  • DOI: 10.1111/his.14210
  • Peer Reviewed
• [Journal Article] Genomic Profiling of Multiple Primary Cancers Including Synchronous Lung Adenocarcinoma and Bilateral Malignant Mesotheliomas: Identification of a Novel BAP1 Germline Variant (2020)
  • Author(s): Shinozaki-Ushiku A, Kohsaka S, Kage H, Oda K, Miyagawa K, Nakajima J, Aburatani H, Mano H, Ushiku T
  • Journal Title: Pathology International Volume: － Issue: 10 Pages: 775-780
  • DOI: 10.1111/pin.12977
  • Peer Reviewed
• [Journal Article] Assessment of Predictive Biomarkers of the Response to Pazopanib Based on an Integrative Analysis of High-grade Soft-tissue Sarcomas: Analysis of a Tumor Sample from a Responder and Patients with Other Soft-tissue Sarcomas (2020)
  • Author(s): Suehara Y.、Kohsaka S.、Yamaguchi S.、Hayashi T.、Kurihara T.、Sano K.、Sasa K.、Akaike K.、Ueno T.、Kojima S.、Ikegami M.、Mizuno S.、Okubo T.、Kim Y.、Kaneko K.、Saito T.、Kato S.、Mano H.
  • Journal Title: Clinical Orthopaedics & Related Research Volume: 478 Issue: 11 Pages: 2461-2476
  • DOI: 10.1097/corr.0000000000001322
  • Peer Reviewed
• [Journal Article] High-throughput functional evaluation of BRCA2 variants of unknown significance. (2020)
  • Author(s): Ikegami, M., Kohsaka, S., Ueno, T., Momozawa, Y., Inoue, S., Tamura, K., Shimomura, A., Hosoya, N., Kobayashi, H., Tanaka, S., Mano, H.
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 2573-2573
  • DOI: 10.1038/s41467-020-16141-8
  • Peer Reviewed / Open Access
• [Journal Article] がんゲノム医療におけるVUS（variant of unknown significance）の評価 (2020)
  • Author(s): 高阪 真路
  • Journal Title: がん分子標的治療 Volume: 18 Pages: 72-77
• [Journal Article] がんゲノム医療の推進に向けた知識データベース構築と維持 (2020)
  • Author(s): 高阪 真路, 小山 隆文, 角南 久仁子, 下井 辰徳, 柴田 大朗
  • Journal Title: 保健医療科学 Volume: 69 Pages: 260-273
  • NAID: 130007926381
  • Open Access
• [Journal Article] Transformation from EGFR/PTEN co-mutated lung adenocarcinoma to small cell carcinoma in lymph node metastasis (2020)
  • Author(s): Hayashi T, Takamochi K, Kohsaka S, Kishikawa S, Suehara Y, Takahashi F, Suzuki K, Saito T, Yao T
  • Journal Title: Pathology International Volume: － Issue: 5 Pages: 295-299
  • DOI: 10.1111/pin.12919
  • Peer Reviewed / Open Access
• [Journal Article] Identification of Novel CD74-NRG2α Fusion From Comprehensive Profiling of Lung Adenocarcinoma in Japanese Never or Light Smokers (2020)
  • Author(s): Kohsaka S, Hayashi T, Nagano M, Ueno T, Kojima S, Kawazu M, Shiraishi Y, Kishikawa S, Suehara Y, Takahashi F, Takahashi K, Suzuki K, Takamochi K, Mano H
  • Journal Title: Journal of Thoracic Oncology Volume: － Issue: 6 Pages: 30098-8
  • DOI: 10.1016/j.jtho.2020.01.021
  • Peer Reviewed / Open Access
• [Journal Article] Mesenchymal Tumors with RREB1-MRTFB Fusion Involving the Mediastinum: Extra-Glossal Ectomesenchymal Chondromyxoid Tumors? (2020)
  • Author(s): Makise N, Mori T, Kobayashi H, Nakagawa K, Ryo E, Nakajima J, Kohsaka S, Mano H, Aburatani H, Yoshida A and Ushiku T
  • Journal Title: Histopathology Volume: － Issue: 7 Pages: 1023-1031
  • DOI: 10.1111/his.14080
  • Peer Reviewed
• [Journal Article] Spinal solitary fibrous tumor of the neck: Next-generation sequencing-based analysis of genomic aberrations. (2020)
  • Author(s): Ando M, Kobayashi H, Shinozaki-Ushiku A, Chikuda H, Matsubayashi Y, Yoshida M, Saito Y, Kohsaka S, Oda K, Miyagawa K, Aburatani H, Mano H, Yamasoba T.
  • Journal Title: Auris Nasus Larynx. Volume: Epub ahead of print Issue: 6 Pages: 1058-1063
  • DOI: 10.1016/j.anl.2019.12.001
  • Peer Reviewed
• [Journal Article] Activation of insulin-like growth factor-1 receptor confers acquired resistance to osimertinib in non-small cell lung cancer with EGFR T790M mutation (2020)
  • Author(s): Hayakawa D, Takahashi F, Mitsuishi Y, Tajima K, Hidayat M, Winardi W, Ihara H, Kanamori K, Matsumoto N, Asao T, Ko R, Shukuya T, Takamochi K, Hayashi T, Suehara Y, Takeda Nakamura I, Ueno T, Kohsaka S, Mano H, Takahashi K
  • Journal Title: Thorac Cancer Volume: 11 Issue: 1 Pages: 140-149
  • DOI: 10.1111/1759-7714.13255
  • Peer Reviewed / Open Access
• [Journal Article] Detection of circulating sarcoma tumor cells using a microfluidic chip-type cell sorter. (2019)
  • Author(s): Hasegawa N, Takeda Nakamura I, Ueno T, Kojima S, Kawazu M, Akaike K, Okubo T, Takagi T, Suehara Y, Hayashi T, Saito T, Kaneko K, Mano H, Kohsaka S.
  • Journal Title: Sci Rep. Volume: 9 Issue: 1 Pages: 20047-20047
  • DOI: 10.1038/s41598-019-56377-z
  • Peer Reviewed / Open Access
• [Journal Article] Protein Expression Profiles Corresponding to Histological Changes with Denosumab Treatment in Giant Cell Tumors of Bone. (2019)
  • Author(s): Suehara Y, Okubo T, Kurihara T, Hayashi T, Kohsaka S, Kazuno S, Sano K, Hasegawa N, Miura Y, Akaike K, Kim Y, Takamochi K, Takahashi F, Ueno T, Kaneko K, Saito T.
  • Journal Title: Proteomics Clin Appl Volume: 13 Issue: 5 Pages: 1800147-1800147
  • DOI: 10.1002/prca.201800147
  • Peer Reviewed
• [Journal Article] Small lung tumor biopsy samples are feasible for high quality targeted next generation sequencing (2019)
  • Author(s): Kage Hidenori、Kohsaka Shinji、Shinozaki‐Ushiku Aya、Hiraishi Yoshihisa、Sato Jiro、Nagayama Kazuhiro、Ushiku Tetsuo、Takai Daiya、Nakajima Jun、Miyagawa Kiyoshi、Aburatani Hiroyuki、Mano Hiroyuki、Nagase Takahide
  • Journal Title: Cancer Science Volume: 110 Issue: 8 Pages: 2652-2657
  • DOI: 10.1111/cas.14112
  • Peer Reviewed / Open Access
• [Journal Article] Comprehensive assay for the molecular profiling of cancer by target enrichment from formalin‐fixed paraffin‐embedded specimens (2019)
  • Author(s): Kohsaka Shinji、Tatsuno Kenji、Ueno Toshihide、et al.
  • Journal Title: Cancer Science Volume: 110 Issue: 4 Pages: 1464-1479
  • DOI: 10.1111/cas.13968
  • Peer Reviewed / Open Access
• [Journal Article] Proteomic signatures corresponding to the SS18/SSX fusion gene in synovial sarcoma. (2018)
  • Author(s): Ishii M, Suehara Y, Sano K, Kohsaka S, Hayashi T, Kazuno S, Akaike K, Mukaihara K, Kim Y, Okubo T, Takamochi K, Takahashi F, Kaneko K, Saito T.
  • Journal Title: Oncotarget Volume: 9(101) Issue: 101 Pages: 37509-37519
  • DOI: 10.18632/oncotarget.26493
  • Peer Reviewed / Open Access
• [Journal Article] Tumor clonality and resistance mechanisms in EGFR mutation-positive non-small-cell lung cancer: implications for therapeutic sequencing (2018)
  • Author(s): Kohsaka S, Petronczki M, Solca F, Maemondo M
  • Journal Title: Future Oncol Volume: 15 Issue: 6 Pages: 637-652
  • DOI: 10.2217/fon-2018-0736
  • Peer Reviewed
• [Journal Article] PPP2R1A regulated by PAX3/FOXO1 fusion contributes to the acquisition of aggressive behavior in PAX3/FOXO1-positive alveolar rhabdomyosarcoma. (2018)
  • Author(s): Akaike K, Suehara Y, Kohsaka S, Hayashi T, Tanabe Y, Kazuno S, Mukaihara K, Toda-Ishii M, Kurihara T, Kim Y, Okubo T, Hayashi Y, Takamochi K, Takahashi F, Kaneko K, Ladanyi M, Saito T.
  • Journal Title: Oncotarget Volume: 9(38) Issue: 38 Pages: 25206-25215
  • DOI: 10.18632/oncotarget.25392
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 発がん関連遺伝子における生殖細胞系列バリアントのハイスループット機能解析法 (2021)
  • Author(s): 高阪真路
  • Organizer: 遺伝性腫瘍セミナー
  • Invited
• [Presentation] Liquid型パネルとC-CATリポジトリの活用 (2021)
  • Author(s): 高阪真路
  • Organizer: 第18回日本臨床腫瘍学会学術集会
  • Invited
• [Presentation] がん個別化医療の実現に向けた戦略的研究開発 (2021)
  • Author(s): 高阪真路
  • Organizer: AI技術が切り拓く新たなリウマチ学に向けた教育シンポジウム
  • Invited
• [Presentation] がんゲノム医療の現況と展望 (2020)
  • Author(s): 高阪真路
  • Organizer: 第33回横浜市立大学がんプロ合同セミナー
  • Invited
• [Presentation] 個別化がんゲノム医療の実現に向けた知識データベースの構築 (2020)
  • Author(s): 高阪真路
  • Organizer: 第58回日本癌治療学会学術集会
  • Invited
• [Presentation] がん個別化医療の実現に向けた戦略的研究開発 (2020)
  • Author(s): 高阪真路
  • Organizer: AI技術が切り拓く新たなリウマチ学に向けた教育シンポジウム
  • Invited
• [Presentation] がんゲノム医療におけるリキッドバイオプシーの実践 (2020)
  • Author(s): 高阪真路
  • Organizer: 第109回日本病理学会総会
  • Invited
• [Presentation] EGFR遺伝子exon 20挿入変異に対するハイスループット機能解析 (2019)
  • Author(s): 廣瀬毅, 高阪真路, 池上政周, 間野博行
  • Organizer: 第42回日本分子生物学会
• [Presentation] がん個別化医療の実現に向けた戦略的研究開発 (2019)
  • Author(s): 高阪真路
  • Organizer: 第237回呼吸器学会関東地方会
  • Invited
• [Presentation] ハイスループット遺伝子機能解析法のがんゲノム医療への応用 (2019)
  • Author(s): 高阪真路
  • Organizer: 第78回日本癌学会学術総会
  • Invited
• [Presentation] シングルセル解析のがんゲノム医療への応用 (2019)
  • Author(s): 高阪真路
  • Organizer: 第16回日本病理学会カンファレンス
  • Invited
• [Presentation] がん遺伝子における意義不明バリアントのハイスループット機能解析法 (2019)
  • Author(s): 高阪真路, 長野匡晃, 上野敏秀, 間野博行
  • Organizer: 第17回日本臨床学会学術集会
  • Invited
• [Presentation] Application of a High-throughput Functional Evaluation of Variants of Unknown Significance to Personalized Medicine (2019)
  • Author(s): 高阪真路
  • Organizer: 第38回札幌国際シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] がん研究からがんゲノム医療への応用 (2019)
  • Author(s): 高阪真路
  • Organizer: 2019年度第1回教育研修・講演会
  • Invited
• [Presentation] ゲノム医療におけるリキッドバイオプシーの有用性 (2019)
  • Author(s): 高阪真路, 間野博行
  • Organizer: 第108回日本病理学会総会
  • Invited
• [Presentation] がんゲノム医療の実現にむけたがん遺伝子ハイスループット機能解析法の構築 (2019)
  • Author(s): 高阪真路
  • Organizer: 第30回日本医学会総会
• [Presentation] がん遺伝子ハイスループット機能解析によるがん生物学の統合的理解 (2018)
  • Author(s): 高阪真路, 長野匡晃, 間野博行
  • Organizer: 第41回日本分子生物学会
  • Invited
• [Presentation] 意義不明変異陽性がんに対する分子標的治療 (2018)
  • Author(s): 高阪真路, 長野匡晃, 間野博行
  • Organizer: 第77回日本癌学会学術総会
  • Invited
• [Presentation] ハイスループット機能解析法のがんゲノム医療への応用 (2018)
  • Author(s): 高阪真路, 長野匡晃, 間野博行
  • Organizer: 第91回日本生化学会大会
  • Invited
• [Presentation] Application of a High-throughput Functional Evaluation of Variants of Unknown Significance to Personalized Medicine (2018)
  • Author(s): Shinji Kohsaka, Masaaki Nagano, and Hiroyuki Mano
  • Organizer: The 6th JCA-AACR Special Joint Conference
  • Int'l Joint Research / Invited
• [Presentation] がんゲノム医療のためのハイスループット機能解析法の確立 (2018)
  • Author(s): 高阪真路, 長野匡晃, 間野博行
  • Organizer: 第107回日本病理学会総会
  • Invited
• [Presentation] NGSのゲノム解析レポートの読み方 (2018)
  • Author(s): 高阪真路
  • Organizer: 第107回日本病理学会総会
  • Invited
• [Presentation] 肺がん関連遺伝子変異のハイスループット機能解析 (2018)
  • Author(s): 高阪真路, 長野匡晃, 間野博行
  • Organizer: 第22回がん分子標的治療学会
  • Invited
• [Presentation] High-throughput functional evaluation of variants of unknown significance in ERBB2 (2018)
  • Author(s): Masaaki Nagano, Shinji Kohsaka, Toshihide Ueno, Shinya Kojima, Masachika Ikegami, Ikuko Takeda, and Hiroyuki Mano
  • Organizer: American Association for Cancer Research Annual Meeting 2018
  • Int'l Joint Research
• [Book] C-CAT調査結果の読み方. がんゲノム医療遺伝子パネル検査実践ガイド (2020)
  • Author(s): 高阪 真路
  • Total Pages: 252
  • Publisher: 医学書院
  • ISBN: 9784260042468
• [Book] 医学のあゆみ がん研究10か年戦略の成果と課題 プレシジョンメディシンの実現に向けた意義不明変異の解釈 (2019)
  • Author(s): 高阪 真路
  • Total Pages: 272
  • Publisher: 医歯薬出版株式会社
• [Book] 病理と臨床 2019年10月号 シングルセル解析遺伝子解析 (2019)
  • Author(s): 高阪 真路
  • Total Pages: 112
  • Publisher: 文光堂
• [Book] BIO Clinica 2019年 8月臨時増刊号 VUS解釈のためのハイスループット機能解析法 (2019)
  • Author(s): 高阪 真路
  • Total Pages: 100
  • Publisher: 北隆館
• [Book] Precision Medicine 2019年2月号 がんクリニカルシークエンス検査の開発と臨床応用 (2019)
  • Author(s): 高阪 真路, 辰野 健二, 織田 克利
  • Total Pages: 102
  • Publisher: 北隆館
• [Book] 特集 がんゲノム医療・Precision Medicineの展開と諸問題 (2018)
  • Author(s): 高阪 真路, 辰野 健二, 織田 克利
  • Total Pages: 132
  • Publisher: 科学評論社
• [Book] 実験医学増刊 Vol.36 No.15 動き始めた がんゲノム医療 (2018)
  • Author(s): 高阪真路
  • Total Pages: 243
  • Publisher: 羊土社
  • ISBN: 9784758103732