| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
The objectives of this study were (1) to elucidate the mode of infection of cultured cells with human prion and the mechanism of persistent infection, and (2) to develop a system for spontaneous prion generation using recombinant prion protein derived from insect cells. After analyzing the growth characteristics and other properties of cultured cells that were used in this study, we verified whether the cells could be infected with human prion persistently. In (2), multiple prion variants were spontaneously generated by the reaction at 45 degrees Celsius, but the variants lost their characteristics and converged to a single one after the reaction repeated. This study clarified the disappearance process of human prion in cultured cells and the mechanism of spontaneous generation of multiple prion variants and their competing processes.
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