Development of recombinant genital herpes vaccine based on the live attenuated rotavirus vaccine
| Project/Area Number |
18H02784
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 52050:Embryonic medicine and pediatrics-related
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
井平 勝 藤田医科大学, 保健学研究科, 教授 (10290165)
三浦 浩樹 藤田医科大学, 医学部, 助教 (10751761)
東本 祐紀 藤田医科大学, 医療科学部, 助教 (20569701)
谷口 孝喜 藤田医科大学, 医学部, 名誉教授 (40094213)
河本 聡志 藤田医科大学, 医学部, 准教授 (60367711)
菅田 健 藤田医科大学, 医学部, 講師 (60454401)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2020: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2019: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Keywords | ロタウイルスワクチン / リバースジェネティックス / 性器ヘルペスワクチン / 粘膜免疫 / リコンビナントワクチン / ロタウイルス / 性器ヘルペス / ワクチン / HSV-2 |
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| Outline of Final Research Achievements |
In this study, we sought to develop simian RV (SA11), in which HSV-2 glycoprotein D (gD2) gene was inserted by reverse genetics system, as a herpes vaccine, and evaluated its immunogenicity in mice.
Artificially synthesized gD2 DNA fragment was inserted into T7 expression NSP1 plasmids (pT7/NSP1-gD2). Using the plasmid, recombinant SA11-gD2 virus (rSA11-gD2) was generated. Insertion of the gD2 gene and synthesis of gD2 protein were demonstrated by sequence analysis and Wester blotting analysis, respectively. Diarrhea occurred after the first inoculation of rSA11-gD2 in suckling mice. Although IgG and IgA antibodies against RV were induced, no gD2 antibody was detected in convalescent sera in the suckling mice. Meanwhile, in the eight-week-old mice inoculated with three times of rSA11-gD2, significant increases in not only IgG and IgA against RV but also IgG against gD2 were demonstrated.
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| Academic Significance and Societal Importance of the Research Achievements |
既に安全性の確立され、定期接種ワクチンとして使用されている弱毒生ロタウイルスワクチンを基盤として、グローバルな観点から公衆衛生学的に重要課題として認識されている性器ヘルペスを予防するためのワクチン開発につながる基盤技術を確立した。新規ワクチンプラットフォームの中でも、性器ヘルペス予防に重要な粘膜免疫を安全かつ効果的に誘導できるものは限られており、本研究成果の意義は極めて高いと考えられる。今後、粘膜免疫が感染防御に重要と考えられる感染症の予防法開発という意味でも、その意義は高い。
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Report
(4 results)
Research Products
(2 results)
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[Journal Article] Persistent systemic rotavirus vaccine infection in a child with X-linked severe combined immunodeficiency.2019
Author(s)
Yoshikawa T, Ihira M, Higashimoto Y, Hattori F, Miura H, Sugata K, Komoto S, Taniguchi K, Iguchi A, Yamada M, Ariga T.
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Journal Title
J Med Virol.
Volume: 91
Issue: 6
Pages: 1008-1013
DOI
Related Report
Peer Reviewed / Open Access
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