Identification of circulating stromal/cancer cell population detected in hepatocellular carcinoma.
Project/Area Number |
18H02792
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 53010:Gastroenterology-related
|
Research Institution | Kanazawa University |
Principal Investigator |
|
Project Period (FY) |
2018-04-01 – 2021-03-31
|
Project Status |
Completed (Fiscal Year 2020)
|
Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
|
Keywords | 肝細胞癌 / 癌幹細胞 / 肝細胞がん / 癌間質細胞 / 肝癌幹細胞 / 肝癌間質細胞 / 循環癌間質細胞 / 1細胞トランスクリプトーム / バイオマーカー |
Outline of Final Research Achievements |
Hepatocellular carcinoma is the second leading cause of cancer death worldwide. In this study, we performed the immunohistochemistry and flow cytometer analysis to identify the cellular heterogeneity of tumor as well as stromal cells. We further performed the single cell transcriptome analysis using peripheral blood mononuclear cells of hepatocellular carcinoma patients. We identified that tumor stromal cells expressing PD1 or CCR6 as well as tumor cells expressing CCL20 were mostly detected at the periphery, not the center of the tumor. We further identified the circulating cell population specifically detected in hepatocellular carcinoma patients. Interestingly, these cell population contained both tumor cells and stromal cells potentially regulating immune system. This study provides important molecular basis of circulating cells potentially be utilized for the diagnosis and treatment of hepatocellular carcinoma.
|
Academic Significance and Societal Importance of the Research Achievements |
肝癌は世界の悪性腫瘍による死因の第2位を占め、新たな診断治療法の開発が強く求められている。本研究では肝癌組織における間質細胞の多様性に注目、特に肝癌患者の血液中に存在する細胞集団に着目、健常人、慢性肝炎、肝硬変患者では存在せず、肝癌患者においてのみ出現する細胞集団を1細胞トランスクリプトーム解析により同定した。これらの細胞集団は肝癌の悪性度や免疫応答制御に関わる細胞集団である可能性がトランスクリプトームデータから示唆されている。今回の研究成果を応用することで新たな高悪性度肝癌の診断、治療の標的分子としての有用性を検討することが可能となり、大きな学術的、社会的意義を有する。
|
Report
(4 results)
Research Products
(8 results)
-
-
[Journal Article] Serum laminin γ2 monomer as a novel diagnostic and predictive biomarker for hepatocellular carcinoma.2021
Author(s)
T Yamashita, N Koshikawa, T Shimakami, T Terashima, M Nakagawa, K Nio, R Horii, N Iida, K Kawaguchi, K Arai, Y Sakai, T Yamashita, E Mizukoshi, M Honda, A Kitao, S Kobayashi, S Takahara, Y Imai, K Yoshimura, T Murayama, Y Nakamoto, E Yoshida, T Yoshimura, M Seiki, S Kaneko.
-
Journal Title
Hepatology
Volume: -
Issue: 2
Pages: 760-775
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] The osteopontin-CD44 axis in hepatic cancer stem cells regulates IFN signaling and HCV replication.2018
Author(s)
Shirasaki T, Honda M, Yamashita T, Nio K, Shimakami T, Shimizu R, Nakasyo S, Murai K, Shirasaki N, Okada H, Sakai Y, Sato T, Suzuki T, Yoshioka K, Kaneko S.
-
Journal Title
Sci Rep.
Volume: 8
Issue: 1
Pages: 13143-13143
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-