Structural basis of neutralized monoclonal antibody which recognizes multiple species of flavivirus
Project/Area Number |
18H02855
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 54030:Infectious disease medicine-related
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Research Institution | High Energy Accelerator Research Organization |
Principal Investigator |
Kato Ryuichi 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (50240833)
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Co-Investigator(Kenkyū-buntansha) |
小澤 龍彦 富山大学, 学術研究部医学系, 准教授 (10432105)
正木 秀幸 近畿大学, 生物理工学部, 准教授 (90247982)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
|
Keywords | ウエストナイルウイルス / フラビウイルス / 中和抗体 / X線結晶構造解析 / 中和モノクローナル抗体 / エンベロープタンパク質 / 立体構造解析 / モノクローナル抗体 / 中和活性 |
Outline of Final Research Achievements |
West Nile virus (WNV) is a mosquito-borne human infectious virus of the Flavivirus family and sometimes causes lethal encephalitis and meningitis. We isolated interesting antibodies which neutralize both WNV and Japanese encephalitis virus (JEV) that also belongs to Flavivirus family. We determined the X-ray crystal structure of the complex of one of the antibodies and the enveloped protein of WNV at 2.6 A resolution. The three-dimensional structure and mutational analysis revealed that Arg388 which are conserved in JEV and WNV and CDR of L-chain are essential for the binding activity.
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Academic Significance and Societal Importance of the Research Achievements |
2種類の抗原に活性を示すモノクローナル抗体の単離自体ユニークであり、それとWNVのエンベロープタンパク質複合体の立体構造解析は当然唯一無二である。明らかにした立体構造から分子認識機構を明らかにすることができ、それはフラビウイルスに共通の抗体医薬および合成低分子抗ウイルス薬の創薬を検討する上で重要な知見を与えると言える。
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Production of a rabbit monoclonal antibody for highly sensitive detection of citrus mosaic virus and related viruses.2020
Author(s)
Miyoshi S, Tokunaga S, Ozawa T, Takeda H, Aono M, Miyoshi T, Kishi H, Muraguchi A, Shimizu S, Nozawa A, Sawasaki T.
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Journal Title
PloS One
Volume: 15
Issue: 4
Pages: e0229196-e0229196
DOI
Related Report
Peer Reviewed / Open Access
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