Long-term culture of human spermatogonial stem cells
| Project/Area Number |
18H02935
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 56030:Urology-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | 精子 / 幹細胞 / 生殖 / 不妊症 / 不妊 |
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| Outline of Final Research Achievements |
Spermatogonial stem cells (SSCs) continuously proliferate by self-renewal division and produce sperm after injection into the seminifelous tubules of testes. This study aimed at the development of new infertility treatment, and tried to develop a method to manipulate human SSCs. CXCR4 was identified as a marker of human SSCs, through screening of cell surface antigens. Factors which promote proliferation of spermatogonia were examined by screening of small molecule chemical libraries and ARTN, NRTN, PSPN, the family molecules of GDNF, the self-renewal factor of SSCs, as well as FGF family molecules, and BMP family molecules, which are considered to be involved in survival of spermatogonia. Using culture conditions modified from the conventional culture condition of mouse SSCs by adding ARTN, a ligand for GFRA3, human testes cells produced colonies in vitro, which were maintained for > 2 months period.
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| Academic Significance and Societal Importance of the Research Achievements |
小児悪性腫瘍患者の生存率は近年向上したが、放射線治療や薬物療法の副作用として不妊になるケースが多い。精子凍結ができない小児患者の妊孕性を担保する方法として、あらかじめ精子幹細胞を凍結保存し治療後に精細管内へ移植する試みが海外にて進められている。しかし小児精巣から採取できる精子幹細胞は極めて少なく、試験管内にて増幅する技術が必要とされている。また培養を行うことで悪性腫瘍細胞の混入を防ぐことができるという利点もある。本研究の成果はヒト精子幹細胞の試験管内増幅技術の確立のために不可欠であるヒト精子幹細胞のマーカー分子や増殖を促す因子を同定した点で、学術的・社会的意義がある。
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] An interplay of NOX1-derived ROS and oxygen determines the spermatogonial stem cell self-renewal efficiency under hypoxia.2021
Author(s)
Hiroko Morimoto, Takuya Yamamoto, Takehiro Miyazaki, Harumi Ogonuki, Atsuo Ogura, Takashi Tanaka, Mito Kanatsu-Shinohara , Chihiro Yabe-Nishimura, Hongliang Zhang, Yves Pommier, Andreas Trumpp, Takashi Shinohara
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Journal Title
Genes Dev.
Volume: 35(3-4)
Issue: 3-4
Pages: 250-260
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Aging of spermatogonial stem cells by Jnk-mediated glycolysis activation.2019
Author(s)
Kanatsu-Shinohara, M., Yamamoto, T., Toh, H., Kazuki, Y., Kazuki, K., Imoto, J., Ikeo, K., Oshima, M., Shirahige, K., Iwama, A., Nabeshima, Y., Sasaki, H. and Shinohara, T.
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Journal Title
PNAS
Volume: 116
Issue: 33
Pages: 16404-16409
DOI
Related Report
Peer Reviewed
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