Development of fertilization therapy for functional recovery of stem cells-wasting pathology
Project/Area Number |
18H02963
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 56070:Plastic and reconstructive surgery-related
|
Research Institution | Jichi Medical University |
Principal Investigator |
|
Project Period (FY) |
2018-04-01 – 2021-03-31
|
Project Status |
Completed (Fiscal Year 2020)
|
Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
|
Keywords | 放射線障害 / 幹細胞 / 老化 / 難治性潰瘍 / 脂肪幹細胞 / 虚血性疾患 / 創傷治癒 / 慢性下肢虚血 |
Outline of Final Research Achievements |
In vitro study using cultured human ASCs found that a single dose of irradiation resulted in a dose-dependent cytotoxicity, but only up to a 2-Gy dose, suggesting the cell cycle stage is critical for determination of susceptibility of stem cells to radiation. The animal study revealed that, in contrast to acute radiation injury, chronic radiation damage-associated wound healing capacity was not proportional to the individual dose or the total irradiation dose received, but appeared to be highly influenced by the number of and the interval between irradiations, if each dose was more than 2 Gy. After radiation exposure, the tissue functions, including vascularity and wound healing capacity, were impaired progressively over time. This result indicated that the tissue devitalization did not result directly from the acute injury; it resulted from the stem cell depletion that was a progressive consequence from repeated irradiation.
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Academic Significance and Societal Importance of the Research Achievements |
本研究結果により、放射線障害の本質は幹細胞死であり、幹細胞枯渇による組織の機能低下であることがわかった。その障害は進行性に増悪するものであり、放射線障害を改善するための、照射プロトコールの開発や予防法の開発に大きく貢献できるものである。
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Report
(4 results)
Research Products
(1 results)