| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2021: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2018: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
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| Outline of Final Research Achievements |
The aim of this study was to develop a technology to induce dentin-pulp complex formation from human iPS cells (hiPS) by using human Copine-7 (hCPNE7) and Semaphorin4D, which regulates cell polarity. MSCs) induced from hiPS and miPS cells were differentiated into odontoblasts when exposed to hCPNE7 or hCPNE7-expressing HAT7 (rat enamel blast cell line) culture supernatant. Furthermore, the basement membrane component secreted by the newly established Hertwig epithelial sheath cell line (HERS02T) also showed high induction of odontblast differentiation and polarity toward mouse incisor papillary mesenchymal stem cells, and similar results were obtained for NCLCs. The combined 3D culture of epithelial cell organoids and NCLC organoids showed enhanced differentiation induction and calcification formation.
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