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Decelopment of nect generation precision theraoy for oral cancer by single cell analysis of oral cancer CTCs

Research Project

Project/Area Number 18H03006
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 57060:Surgical dentistry-related
Research InstitutionKagoshima University

Principal Investigator

SUGIURA TSUYOSHI  鹿児島大学, 医歯学域歯学系, 教授 (40322292)

Co-Investigator(Kenkyū-buntansha) 加藤 護  国立研究開発法人国立がん研究センター, 研究所, 部門長 (40391916)
高田 耕児  富山県産業技術研究開発センター, その他部局等, 主任研究員 (40530621)
新井 康仁  国立研究開発法人国立がん研究センター, 研究所, 主任研究員 (80222727)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywords口腔癌 / 早期診断 / 腫瘍マーカー / 循環癌細胞 / マイクロRNA / 循環癌細胞 早期診断 / 次世代シーケンス / 口腔がん / シングルセル解析 / 次世代シーケンサー
Outline of Final Research Achievements

Circulating tumor cell plays crucial role in cancer metastasis. In this project, we developed two important techniques for oral cancer early detection and treatment.
1.We developed CTC separating deveice using size sort chip and its separating performance is around 99% purity from peripheral blood.
2. We developed microRNA based diagnosis system for oral cancer using perpheral blood sample from oral cacner patients. We newly defined "microRNA index for oral cancer detection" using six specific miRNAs(miR-24,miR-20a,miR-122,miR-150,miR-4419a,miR-5100) and it diagnosed oral cancer by sensitivity 55% and specificity 92.5%.

Academic Significance and Societal Importance of the Research Achievements

6つの血清中miRNA(miR-24,miR-20a,miR-122,miR-150,miR-4419a,miR-5100)を組み合わせたmiRNA indexは、従来もちいられていた腫瘍マーカーと比較しても診断能が極めて高いことが示された。これらのmiRNAは細胞内シグナルに重要な分子を標的にすることが明らかになっており、その標的分子は多くの癌腫における悪性化の指標となっていることから今後治療への応用が期待できる。

Report

(4 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (7 results)

All 2021 2020 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (4 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Circulating microRNA Panel as a Potential Novel Biomarker for Oral Squamous Cell Carcinoma Diagnosis2021

    • Author(s)
      Kodai Nakamura, Naomi Hiyake, Tomofumi Hamada, Seiya Yokoyama, Kazuki Mori, Kouta Yamashiro, Mahiro Beppu, Yasuaki Sagara, Yoshiaki Sagara, Tsuyoshi Sugiura
    • Journal Title

      Cancers

      Volume: 13(3) Issue: 3 Pages: 449-449

    • DOI

      10.3390/cancers13030449

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Noninvasive precision diagnosis and real-time monitoring for oral cancer based on patient’s time axis2020

    • Author(s)
      Sugiura Tsuyoshi
    • Journal Title

      Journal of Japanese Society of Oral Oncology

      Volume: 32 Issue: 4 Pages: 153-158

    • DOI

      10.5843/jsot.32.153

    • NAID

      130007958730

    • ISSN
      0915-5988, 1884-4995
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 患者時間軸を重視した非侵襲口腔癌精密診断とリアルタイムモニタリング2020

    • Author(s)
      杉浦剛
    • Organizer
      第38回 日本口腔腫瘍学会総会・学術大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] マイクロ流体チップによるCTC の検出2019

    • Author(s)
      松村吉晃、杉浦剛
    • Organizer
      第37回日本口腔腫瘍学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] マイクロ流体チップによるCTC の検出2018

    • Author(s)
      松村吉晃、杉浦剛
    • Organizer
      第63 回日本口腔外科学会総会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Capture of CTC using SS-Chip2018

    • Author(s)
      松村吉晃、杉浦剛
    • Organizer
      第77 回日本癌学会学術総会
    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] 口腔癌判定装置、口腔癌判定方法、プログラム及び口腔癌判定キット2018

    • Inventor(s)
      中村康大、杉浦剛、浜田倫史
    • Industrial Property Rights Holder
      中村康大、杉浦剛、浜田倫史
    • Industrial Property Rights Type
      特許
    • Patent Publication Number
      2020-068673
    • Filing Date
      2018
    • Acquisition Date
      2020
    • Related Report
      2020 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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