• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of cell fate determination based on transcriptome analysis in tooth development

Research Project

Project/Area Number 18H03012
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 57070:Developmental dentistry-related
Research InstitutionKyushu University

Principal Investigator

Yoshizaki Keigo  九州大学, 歯学研究院, 助教 (10507982)

Co-Investigator(Kenkyū-buntansha) 福本 敏  九州大学, 歯学研究院, 教授 (30264253)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Keywords歯 / 上皮-間葉相互作用 / CAGE / 形態形成 / 再生医学 / 発現解析 / miRNA / 咬頭形成
Outline of Final Research Achievements

Tooth morphogenesis is initiated by reciprocal interactions between the ectoderm and neural crest derived mesenchyme. During tooth development, tooth cusps are regulated by precise control of proliferation of cell clusters, termed enamel knots, that are present among dental epithelial cells. However, the mechanisms of tooth formation are still unclear. In this study, we performed CAGE analysis using early stages of tooth germ, and identified Nkx2-3, which may regulates tooth morphogenesis. We also demonstrate that Nkx2-3 is a target molecule of EDA and critical for expression of the cell cycle regulator p21 in the enamel knot.

Academic Significance and Societal Importance of the Research Achievements

歯、毛、唾液腺、肺および腎臓など、上皮-間葉相互作用によって形成される器官は、その複雑な形成過程から、再生は未だ困難な状況にある。これは、2種類以上の細胞の組み合わせによる形態形成の難しさを示しており、形態形成期における制御機構の解明が期待されていた。本研究では、歯、毛、唾液腺、肺および腎臓の初期発生に関わるトランスクリプトーム解析をCAGE法を用いて行い、新規形態形成因子の検索を行い、歯の咬頭形成に重要である転写因子の同定に成功した。本研究成果は、再生器官へ正しい形態を付与する技術へ応用することが可能であり、将来の器官再生技術への応用が期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (34 results)

All 2020 2019 2018

All Journal Article (11 results) (of which Int'l Joint Research: 10 results,  Peer Reviewed: 11 results,  Open Access: 11 results) Presentation (23 results) (of which Int'l Joint Research: 6 results)

  • [Journal Article] Sox21 Regulates Anapc10 Expression and Determines the Fate of Ectodermal Organ2020

    • Author(s)
      Saito K, Michon F, Yamada A, Inuzuka H, Yamaguchi S, Fukumoto E, Yoshizaki K, Nakamura T, Arakaki M, Chiba Y, Ishikawa M, Okano H, Thesleff I, Fukumoto S.
    • Journal Title

      iScience

      Volume: 23 Issue: 7 Pages: 101329-101329

    • DOI

      10.1016/j.isci.2020.101329

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] G protein coupled receptor Gpr115 (Adgrf4) is required for enamel mineralization mediated by ameloblasts2020

    • Author(s)
      Chiba Yuta、Yoshizaki Keigo、Saito Kan、Ikeuchi Tomoko、Iwamoto Tsutomu、Rhodes Craig、Nakamura Takashi、de Vega Susana、Morell Robert J.、Boger Erich T.、Martin Daniel、Hino Ryoko、Inuzuka Hiroyuki、Bleck Christopher K.E.、Yamada Aya、Yamada Yoshihiko、Fukumoto Satoshi
    • Journal Title

      Journal of Biological Chemistry

      Volume: 295 Issue: 45 Pages: 15328-15341

    • DOI

      10.1074/jbc.ra120.014281

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Melnick-Needles syndrome associated molecule, Filamin-A regulates dental epithelial cell migration and root formation2020

    • Author(s)
      Hino Ryoko、Yamada Aya、Chiba Yuta、Yoshizaki Keigo、Fukumoto Emiko、Iwamoto Tsutomu、Maruya Yuriko、Otsu Keishi、Harada Hidemistu、Saito Kan、Fukumoto Satoshi
    • Journal Title

      Pediatric Dental Journal

      Volume: 30 Issue: 3 Pages: 208-214

    • DOI

      10.1016/j.pdj.2020.09.003

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Single-Cell RNA-Sequencing From Mouse Incisor Reveals Dental Epithelial Cell-Type Specific Genes2020

    • Author(s)
      Chiba Y, Saito K, Martin D, Boger ET, Rhodes C, Yoshizaki K, Nakamura T, Yamada A, Morell RJ, Yamada Y, Fukumoto S.
    • Journal Title

      Front Cell Dev Biol

      Volume: 8 Pages: 841-841

    • DOI

      10.3389/fcell.2020.00841

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Expression Patterns of Claudin Family Members During Tooth Development and the Role of Claudin-10 (Cldn10) in Cytodifferentiation of Stratum Intermedium2020

    • Author(s)
      Wang Xin、Chiba Yuta、Jia Lingling、Yoshizaki Keigo、Saito Kan、Yamada Aya、Qin Man、Fukumoto Satoshi
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 8 Pages: 595593-595593

    • DOI

      10.3389/fcell.2020.595593

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mouse embryonic tooth germ dissection and ex vivo culture protocol.2020

    • Author(s)
      Han X, Yoshizaki K, Tian T, Miyazaki K, Takahashi I, Fukumoto S.
    • Journal Title

      Bio-protocol

      Volume: 10 Issue: 3

    • DOI

      10.21769/bioprotoc.3515

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] microRNA-875-5p plays critical role for mesenchymal condensation in epithelial-mesenchymal interaction during tooth development2020

    • Author(s)
      Funada Keita、Yoshizaki Keigo、MIyazaki Kanako、Han Xue、Yuta Tomomi、Tian Tian、Mizuta Kanji、Fu Yao、Iwamoto Tsutomu、Yamada Aya、Takahashi Ichiro、Fukumoto Satoshi
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 4918-4918

    • DOI

      10.1038/s41598-020-61693-w

    • NAID

      120006872357

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Coordination of WNT signaling and ciliogenesis during odontogenesis by piezo type mechanosensitive ion channel component 1.2019

    • Author(s)
      Miyazaki A, Sugimoto A, Yoshizaki K, Kawarabayashi K, Iwata K, Kurogoushi R, Kitamura T, Otsuka K, Hasegawa T, Akazawa Y, Fukumoto S, Ishimaru N, Iwamoto T.
    • Journal Title

      Sci Rep.

      Volume: 9(1) Issue: 1 Pages: 14762-14762

    • DOI

      10.1038/s41598-019-51381-9

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The transcription factor AmeloD stimulates epithelial cell motility essential for tooth morphology.2019

    • Author(s)
      Chiba Y, He B, Yoshizaki K, Rhodes C, Ishijima M, Bleck CKE, Stempinski E, Chu EY, Nakamura T, Iwamoto T, de Vega S, Saito K, Fukumoto S, Yamada Y
    • Journal Title

      J Biol Chem

      Volume: 294(10) Issue: 10 Pages: 3406-3418

    • DOI

      10.1074/jbc.ra118.005298

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The transcription factor NKX2-3 mediates p21 expression and ectodysplasin-A signaling in the enamel knot for cusp formation in tooth development2018

    • Author(s)
      Han Xue、Yoshizaki Keigo、Miyazaki Kanako、Arai Chieko、Funada Keita、Yuta Tomomi、Tian Tian、Chiba Yuta、Saito Kan、Iwamoto Tsutomu、Yamada Aya、Takahashi Ichiro、Fukumoto Satoshi
    • Journal Title

      Journal of Biological Chemistry

      Volume: 293 Issue: 38 Pages: 14572-14584

    • DOI

      10.1074/jbc.ra118.003373

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of the Novel Tooth-Specific Transcription Factor AmeloD2018

    • Author(s)
      He B.、Chiba Y.、Li H.、de Vega S.、Tanaka K.、Yoshizaki K.、Ishijima M.、Yuasa K.、Ishikawa M.、Rhodes C.、Sakai K.、Zhang P.、Fukumoto S.、Zhou X.、Yamada Y.
    • Journal Title

      Journal of Dental Research

      Volume: 98 Issue: 2 Pages: 234-241

    • DOI

      10.1177/0022034518808254

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 歯特異的microRNAによる上皮-間葉相互作用制御機構の解明2020

    • Author(s)
      吉崎 恵悟、福本 敏
    • Organizer
      第58回日本小児歯科学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 成熟期エナメル芽細胞におけるGタンパク質共役型受容体の機能解析2020

    • Author(s)
      千葉 雄太、斎藤 幹、吉崎 恵悟、福本 敏
    • Organizer
      第62回歯科基礎医学会学術大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Cyclophosphamide, an anti-cancer drug, disrupts tooth germ development by inhibiting epithelial cell proliferation and differentiation.2020

    • Author(s)
      Tian Tian, Keigo Yosizaki, Kanako Miyazaki, Keita Funada, Tomomi Yuta, Kanji Mizuta, Yao Fu, Ichiro Takahashi.
    • Organizer
      第9回国際矯正歯科会議世界大会
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nephronectin regulates ameloblast differentiation through its RGD domain.2020

    • Author(s)
      Kanji Mizuta, Keigo Yoshizaki, Kanako Miyazaki, Keita Funada, Tomomi Yuta, Tian Tian, Yao Fu, Ichiro Takahashi.
    • Organizer
      第9回国際矯正歯科会議世界大会
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A tooth specific Lypd1 expressed in dental mesenchyme regulates odontoblast differentiation in tooth development.2020

    • Author(s)
      Yao Fu, Kanako Miyazaki, Keigo Yoshizaki, Keita Funada, Tomomi Yuta, Tian Tian, Kanji Mizuta, Ichiro Takahashi.
    • Organizer
      第9回国際矯正歯科会議世界大会
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of the gene regulation of the novel inner enamel epithelium marker AmeloD.2019

    • Author(s)
      Shahad AI Thamin, Yuta Chiba, Keigo Yoshizaki, LingLing Jia, Aya Yamada, Kan Saito, Satoshi Fukumoto.
    • Organizer
      第57回日本小児歯科学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 歯原性上皮幹細胞の分離および転写因子NKX2-3の咬頭形成における作用2019

    • Author(s)
      韓雪、吉崎恵悟、宮崎佳奈子、田甜、高橋一郎、福本敏
    • Organizer
      2019口腔医学青年科学家討論大会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gタンパク質共役型受容体Gpr115はエナメル芽細胞における炭酸脱水素酵素の発言を制御しエナメル質形成に関与する2019

    • Author(s)
      葉 雄太, 齋藤 幹, 吉崎 恵悟, 福本 敏
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The G-protein coupled receptor Gpr115 regulates expression of the carbonic anhydrase in ameloblast during enamel formation.2019

    • Author(s)
      Chiba Y, Saito K, Yoshizaki K, Fukumoto S.
    • Organizer
      第61回歯科基礎医学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The G-protein coupled receptor Gpr115 is essential for enamel mineralization via regulation of pH homeostasis.2019

    • Author(s)
      Yuta Chiba, Keigo Yoshizaki, Tomoko Ikeuchi, Kan Saito, Craig Rhodes, Tsutomu Iwamoto, Takashi Nakamura, Yoshihiko Yamada and Satoshi Fukumoto.
    • Organizer
      NIH-Japan-JSPS symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PKP1の歯原性上皮細胞における密着結合因子ZO-1の局在制御機構2019

    • Author(s)
      宮崎佳奈子、吉崎恵悟、傅堯、韓雪、鮒田啓太、田甜、湯田智美、水田敢士、高橋一郎
    • Organizer
      第78回日本矯正歯科学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] microRNA-875-5pはPDGFシグナル経路を介して歯の上皮-間葉相互作用を制御する2019

    • Author(s)
      鮒田啓太、吉崎恵悟、宮崎佳奈子、韓雪、田甜、湯田智美、水田敢士、傅堯、高橋一郎
    • Organizer
      第78回日本矯正歯科学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] microRNA875-5pは歯の発生においてPDGFシグナルを介して上皮-間葉相互作用を制御する2019

    • Author(s)
      鮒田啓太、吉崎恵悟、宮崎佳奈子、韓雪、湯田智美、田甜、水田敢士、傅堯、福本敏、高橋一郎
    • Organizer
      第42回日本分子生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Nkx2-3は歯の発生においてEDA/EDARシグナル伝達経路を介してp21の転写制御を行い歯の咬頭形成を制御する2019

    • Author(s)
      田甜、吉崎恵悟、韓雪、宮崎佳奈子、鮒田啓太、湯田智美、水田敢士、傅堯、福本敏、高橋一郎
    • Organizer
      第42回日本分子生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] デスモゾーム構成因子PKP1は歯原性上皮細胞の密着結合においてZO-1の局在を制御する2019

    • Author(s)
      堯、宮崎佳奈子、吉崎恵悟、湯田智美、韓雪、鮒田啓太、田甜、水田敢士、福本敏、高橋一郎
    • Organizer
      第42回日本分子生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] PKP1 は歯原性上皮細胞において密着結合構成因子 ZO-1 の局在を制御する2018

    • Author(s)
      宮崎佳奈子、吉崎恵悟、湯田智美、韓雪、新井智映子、鮒田啓太、田甜 、福本敏、高橋一郎
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 歯に特異的に発現する microRNA-875 の同定および歯の発生における役割2018

    • Author(s)
      鮒田啓太、吉崎恵悟、韓雪、宮崎佳奈子、新井智映子、湯田智美、田甜 、福本敏、高橋一郎
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 歯の咬頭形成におけるホメオボックス転写因子 Nkx2-3 の役割2018

    • Author(s)
      韓雪、吉崎恵悟、宮崎佳奈子、新井智映子、鮒田啓太、湯田智美、田甜 、福本敏、高橋一郎
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 新規basic-helix-loop-helix転写因子AmeloDは歯原性上皮細胞の遊走能を制御し歯胚形態形成に関与する2018

    • Author(s)
      千葉雄太、吉崎恵悟、齋藤幹、中村卓史、岩本勉、福本敏
    • Organizer
      第60回歯科基礎医学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The G protein-coupled receptor Gpr115 regulates pH homeostasis that is essential for tooth mineralization2018

    • Author(s)
      Yuta Chiba, Kan Saito, Keigo Yoshizaki, Tsutomu Iwamoto, Yoshihiko Yamada and Satoshi Fukumoto
    • Organizer
      11th Pediatric Dentistry Association of Asia
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 歯特異的microRNA-875は歯の発生において歯原性間葉細胞の凝集を誘導する2018

    • Author(s)
      鮒田啓太、吉崎恵悟、韓雪、宮崎佳奈子、新井智映子、湯田智美、田甜、高橋一郎
    • Organizer
      第77回日本矯正歯科学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ホメオボックス転写因子Nkx2-3による歯の咬頭形成制御機構2018

    • Author(s)
      田甜 、吉崎恵悟、韓雪、宮崎佳奈子、新井智映子、鮒田啓太、湯田智美、高橋一郎
    • Organizer
      第77回日本矯正歯科学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 下顎頭軟骨の分化を制御するmechanosensitive miRNA の探索2018

    • Author(s)
      野田 佳苗, 星 健治, 吉崎 恵悟, 髙橋 一郎
    • Organizer
      第14回九州矯正歯科学会学術大会
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi