| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Outline of Final Research Achievements |
Expression levels of nicotinamide N-methyltransferase (NNMT) mRNA are diminished in the liver of glucagon gene knock-out mouse (GCGKO). As amino acid metabolism is largely altered in GCGKO, we explored possible involvement of NNMT in regulation of amino acid metabolism, as well as glucose metabolism.
To this end, we generated mouse models which lack NNMT ubiquitously or in a liver-specific manner (NNMTKO and NNMTLKO, respectively). We also crossed these models with GCGKO to produce double knockout models for NNMT and GCGKO (NNMT/GCG DKO).
NNMTKO, NNMTLKO and NNMT/GCGDKO were born according to expected ratio, indicating that NNMT is not absolutely required for development and survival. No significant difference in the expression levels of genes involved in metabolism of amino acids and glucose was observed between the control and KO mice. Therefore, NNMT plays relatively minor role in the regulation of amino acid metabolism, at least under normal or disease-free conditions.
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