Elucidation of translation-arresting sequences in genomes using computer-based intersectional analyses of life science databases

• Project/Area Number: 18H03330
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 62010:Life, health and medical informatics-related
• Research Institution: Kanazawa University
• Principal Investigator: 高橋 広夫 金沢大学, 薬学系, 准教授 (30454367)
• Co-Investigator(Kenkyū-buntansha): 伊藤 素行 千葉大学, 大学院薬学研究院, 教授 (20377906) ; 尾之内 均 北海道大学, 農学研究院, 准教授 (50322839) ; 佐々木 博己 国立研究開発法人国立がん研究センター, 研究所, 部門長 (60235265) ; 町田 泰則 名古屋大学, 理学研究科, 名誉教授 (80175596)
• Project Period (FY): 2018 – 2020
• Project Status: Discontinued (Fiscal Year 2020)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2018: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: バイオインフォマティクス / 翻訳アレスト / 非コード領域 / オミックス / 翻訳制御 / データベース横断解析 / 新生鎖

Outline of Annual Research Achievements

Upstream Open Reading Frame (uORF)は、AUG以外の開始コドンから翻訳が始まる非AUG開始型uORFが存在する。そのような非AUG開始型uORFの中で翻訳アレストを起こすものを同定するために、ESUCAパイプラインを改変して進化的に保存された配列を持つ非AUG開始型uORFを動植物のゲノムから探索した。その結果、そのような非AUG開始型uORFが14個同定された。それらの中から、主要ORFの翻訳制御に関与するものを一過的発現解析により4個同定した。また、胃がん発症機構解明のため、本年度は、高度遺伝子増幅領域内の遺伝子発現がプロモーターのメチル化と関連しているか、メチロームデータとの3層オミクス統合解析を行い、遺伝子の発現量とメチル化の間に、強い相関があることを見出した。遺伝子増幅に伴って過剰発現する遺伝子にはGATA6のような新たながん遺伝子が多く、発現が低下している遺伝子にはGSDMAのようながん抑制遺伝子が含まれていた。これらにより、3層オミクス統合解析によって、腹膜転移性胃がんの増幅遺伝子の全体像が明らかになった。

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] Comprehensive genome-wide identification of angiosperm upstream ORFs with peptide sequences conserved in various taxonomic ranges using a novel pipeline, ESUCA (2020)
  • Author(s): Takahashi Hiro、Hayashi Noriya、Hiragori Yuta、Sasaki Shun、Motomura Taichiro、Yamashita Yui、Naito Satoshi、Takahashi Anna、Fuse Kazuyuki、Satou Kenji、Endo Toshinori、Kojima Shoko、Onouchi Hitoshi
  • Journal Title: BMC Genomics Volume: 21 Issue: 1 Pages: 260-260
  • DOI: 10.1186/s12864-020-6662-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Excess sterols disrupt plant cellular activity by inducing stress-responsive gene expression (2020)
  • Author(s): Shimada Takashi L、Yamaguchi Katsuki、Shigenobu Shuji、Takahashi Hiro、Murase Masataka、Fukuyoshi Shuichi、Hara-Nishimura Ikuko
  • Journal Title: Journal of Plant Research Volume: in press Issue: 3 Pages: 383-392
  • DOI: 10.1007/s10265-020-01181-4
  • Peer Reviewed
• [Journal Article] Draft Genome Sequence of Aspergillus terreus High-Itaconic-Acid-Productivity Strain IF06365 (2020)
  • Author(s): Takahashi H., Minami T., Okabe M., Park E. Y., Fujimoto T., Takahashi A., Murase M., Fukuyoshi S., Satou K., Kanamasa S.
  • Journal Title: Microbiology Resource Announcements
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Efficacy of glutathione inhibitors for the treatment of ARID1A-deficient diffuse-type gastric cancers (2020)
  • Author(s): Sasaki M., Chiwaki F., Kuroda T., Komatsu M., Matsusaki K., Kohno T., Sasaki H., Ogiwara H.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 522 Issue: 2 Pages: 342-347
  • DOI: 10.1016/j.bbrc.2019.11.078
  • Peer Reviewed
• [Journal Article] CD24 and CK4 are upregulated by SIM2, and are predictive biomarkers for chemoradiotherapy and surgery in esophageal cancer. (2020)
  • Author(s): Takashima K, Fujii S, Komatsuzaki R, Komatsu M, Takahashi M, Kojima T, Daiko H, Minashi K, Chiwaki F, Muto M, Sasaki H, Yano T.
  • Journal Title: Int J Oncol. Volume: 56 Pages: 835-847
  • DOI: 10.3892/ijo.2020.4963
  • Peer Reviewed / Open Access
• [Journal Article] Bacterial SOS Genes mucAB/umuDC Promote Mouse Tumors by Activating Oncogenes Nedd9/Aurkb via a miR-145 Sponge (2020)
  • Author(s): Tanooka H, Inoue A, Takahashi RU, Tatsumi K, Fujikawa K, Nagao T, Ishiai M, Chiwaki F, Aoyagi K, Sasaki H, Ochiya T.
  • Journal Title: Mol Cancer Res. Volume: 18 Issue: 9 Pages: 1271-1277
  • DOI: 10.1158/1541-7786.mcr-20-0137
  • Peer Reviewed
• [Journal Article] Report of the use of patient‐derived xenograft models in the development of anticancer drugs in Japan (2020)
  • Author(s): Tsumura Ryo、Koga Yoshikatsu、Hamada Akinobu、Kuwata Takeshi、Sasaki Hiroki、Doi Toshihiko、Aikawa Katsuji、Ohashi Akihiro、Katano Ikumi、Ikarashi Yoshinori、Ito Mamoru、Ochiai Atsushi
  • Journal Title: Cancer Science Volume: 111 Issue: 9 Pages: 3386-3394
  • DOI: 10.1111/cas.14564
  • Peer Reviewed / Open Access
• [Journal Article] Molecular Network Profiling in Intestinal- and Diffuse-Type Gastric Cancer. (2020)
  • Author(s): Tanabe S, Quader S, Ono R, Cabral H, Aoyagi K, Hirose A, Yokozaki H, Sasaki H
  • Journal Title: Cancers Volume: 12 Issue: 12 Pages: 3833-3833
  • DOI: 10.3390/cancers12123833
  • Peer Reviewed / Open Access
• [Journal Article] Exhaustive identification of conserved upstream open reading frames with potential translational regulatory functions from animal genomes (2020)
  • Author(s): Takahashi Hiro、Miyaki Shido、Onouchi Hitoshi、Motomura Taichiro、Idesako Nobuo、Takahashi Anna、Murase Masataka、Fukuyoshi Shuichi、Endo Toshinori、Satou Kenji、Naito Satoshi、Itoh Motoyuki
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 16289-16289
  • DOI: 10.1038/s41598-020-73307-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The formation of perinucleolar bodies is important for normal leaf development and requires the zinc‐finger DNA‐binding motif in Arabidopsis ASYMMETRIC LEAVES2 (2020)
  • Author(s): Luo Lilan、Ando Sayuri、Sakamoto Yuki、Suzuki Takanori、Takahashi Hiro、Ishibashi Nanako、Kojima Shoko、Kurihara Daisuke、Higashiyama Tetsuya、Yamamoto Kotaro T.、Matsunaga Sachihiro、Machida Chiyoko、Sasabe Michiko、Machida Yasunori
  • Journal Title: The Plant Journal Volume: 101 Issue: 5 Pages: 1118-1134
  • DOI: 10.1111/tpj.14579
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reverse genetics-based biochemical studies of the ribosomal exit tunnel constriction region in eukaryotic ribosome stalling: spatial allocation of the regulatory nascent peptide at the constriction. (2020)
  • Author(s): Seidai Takamatsu, Yubun Ohashi, Noriyuki Onoue, Yoko Tajima, Tomoya Imamichi, Shinya Yonezawa, Kyoko Morimoto, Hitoshi Onouchi, Yui Yamashita, Satoshi Naito
  • Journal Title: Nucleic Acids Res. Volume: 48 Issue: 4 Pages: 1985-1999
  • DOI: 10.1093/nar/gkz1190
  • Peer Reviewed / Open Access
• [Journal Article] Draft Genome Sequence of Aspergillus terreus High-Itaconic-Acid- Productivity Strain IFO6365 (2020)
  • Author(s): Takahashi H., Minami T., Okabe M., Park E.Y., Fujimoto T., Takahashi A., Murase M., Fukuyoshi S., Satou K., and Kanamasa S.
  • Journal Title: Microbiol. Resour. Announc. Volume: - Issue: 16 Pages: 1-2
  • DOI: 10.1128/mra.00080-20
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Actin Filament Disruption Alters Phragmoplast Microtubule Dynamics during the Initial Phase of Plant Cytokinesis (2020)
  • Author(s): Maeda Keisho、Sasabe Michiko、Hanamata Shigeru、Machida Yasunori、Hasezawa Seiichiro、Higaki Takumi
  • Journal Title: Plant and Cell Physiology Volume: 61 Issue: 3 Pages: 445-456
  • DOI: 10.1093/pcp/pcaa003
  • Peer Reviewed / Open Access
• [Journal Article] SIMON: Simple methods for analyzing DNA methylation by targeted bisulfite next-generation sequencing (2019)
  • Author(s): Vial-Pradel Simon、Hasegawa Yoshinori、Nakagawa Ayami、Miyaki Shido、Machida Yasunori、Kojima Shoko、Machida Chiyoko、Takahashi Hiro
  • Journal Title: Plant Biotechnology Volume: 36 Issue: 4 Pages: 213-222
  • DOI: 10.5511/plantbiotechnology.19.0822a
  • NAID: 130007776104
  • ISSN: 1342-4580, 1347-6114
  • Year and Date: 2019-12-25
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Patient‐derived xenograft models of non‐small cell lung cancer for evaluating targeted drug sensitivity and resistance (2019)
  • Author(s): Kenji Kita, Koji Fukuda, Hiro Takahashi, Azusa Tanimoto, Akihiro Nishiyama, Sachiko Arai, Shinji Takeuchi, Kaname Yamashita, Koshiro Ohtsubo, Sakiko Otani, Naohiro Yanagimura, Chiaki Suzuki, Hiroko Ikeda, Masaya Tamura, Isao Matsumoto, Seiji Yano
  • Journal Title: Cancer Science Volume: 110(10) Issue: 10 Pages: 3215-3224
  • DOI: 10.1111/cas.14171
  • Peer Reviewed / Open Access
• [Journal Article] Draft Genome Sequence of Saccharomyces cerevisiae Strain Pf-1, Isolated from Prunus mume (2019)
  • Author(s): Kanamasa Shin、Yamaguchi Daiki、Machida Chiyoko、Fujimoto Tsukasa、Takahashi Anna、Murase Masataka、Fukuyoshi Shuichi、Oda Akifumi、Satou Kenji、Takahashi Hiro
  • Journal Title: Microbiology Resource Announcements Volume: 8 Issue: 46
  • DOI: 10.1128/mra.01169-19
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Draft Genome Sequence of Aspergillus terreus High-Itaconic-Acid-Productivity Mutant TN-484 (2019)
  • Author(s): Kanamasa Shin、Minami Tomoyuki、Okabe Mitsuyasu、Park Enoch Y.、Fujimoto Tsukasa、Takahashi Anna、Murase Masataka、Fukuyoshi Shuichi、Oda Akifumi、Satou Kenji、Takahashi Hiro
  • Journal Title: Microbiology Resource Announcements Volume: 8 Issue: 49
  • DOI: 10.1128/mra.01170-19
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Targeting the Vulnerability of Glutathione Metabolism in ARID1A-Deficient Cancers. (2019)
  • Author(s): Ogiwara H, Takahashi K, Sasaki M, Kuroda T, Yoshida H, Watanabe R, Maruyama A, Makinoshima H, Chiwaki F, Sasaki H, Kato T, Okamoto A, Kohno T.
  • Journal Title: Cancer Cell Volume: 35 Issue: 2 Pages: 177-190
  • DOI: 10.1016/j.ccell.2018.12.009
  • Peer Reviewed / Open Access
• [Journal Article] Upregulation of IGF2R evades lysosomal dysfunction-induced apoptosis of cervical cancer cells via transport of cathepsins (2019)
  • Author(s): Takeda Takashi, Komatsu Masayuki, Chiwaki Fumiko, Komatsuzaki Rie, Nakamura Kanako, Tsuji Kosuke, Kobayashi Yusuke, Tominaga Eiichiro, Ono Masaya, Banno Kouji, Aoki Daisuke, Sasaki Hiroki.
  • Journal Title: Cell Death & Disease Volume: 10(876) Issue: 12 Pages: 1-17
  • DOI: 10.1038/s41419-019-2117-9
  • Peer Reviewed / Open Access
• [Journal Article] バイオインフォマティクスによる配列データベース横断解析に基づく機能上流ORFの推定 (2019)
  • Author(s): 高橋広夫・伊藤素行・尾之内均
  • Journal Title: 生物工学会誌 Volume: 97 Pages: 496-499
• [Journal Article] A newly developed feeder and oxygen measurement system reveals the effects of aging and obesity on the metabolic rate of zebrafish (2019)
  • Author(s): Yang Peng、Yamaki Misato、Kuwabara Shuhei、Kajiwara Riki、Itoh Motoyuki
  • Journal Title: Experimental Gerontology Volume: 127 Pages: 110720-110720
  • DOI: 10.1016/j.exger.2019.110720
  • Peer Reviewed
• [Journal Article] Arabidopsis Zinc-Finger-Like Protein ASYMMETRIC LEAVES2 (AS2) and Two Nucleolar Proteins Maintain Gene Body DNA Methylation in the Leaf Polarity Gene ETTIN (ARF3) (2018)
  • Author(s): Vial-Pradel Simon、Keta Sumie、Nomoto Mika、Luo Lilan、Takahashi Hiro、Suzuki Masataka、Yokoyama Yuri、Sasabe Michiko、Kojima Shoko、Tada Yasuomi、Machida Yasunori、Machida Chiyoko
  • Journal Title: Plant and Cell Physiology Volume: Epub ahead of print Pages: 1385-1397
  • DOI: 10.1093/pcp/pcy031
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Draft Genome Sequence of Saccharomyces cerevisiae Strain Hm-1, Isolated from Cotton Rosemallow (2018)
  • Author(s): Takahashi H., Sakagawa E., Sakakibara I., Machida C., Miyaki S., Takahashi A., Onai S., Fukuyoshi S., Ohta A., Satou K., Kanamasa S.
  • Journal Title: Microbiology Resource Announcements Volume: 7 Issue: 13 Pages: 1-2
  • DOI: 10.1128/mra.01184-18
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Draft Genome Sequence of Trebouxiophyceae sp. Strain KSI-1, Isolated from an Island Hot Spring (2018)
  • Author(s): Takahashi H., Tanaka S., Hayashi S., Miyaki S., Takahashi A., Onai S., Fukuyoshi S., and Miyasaka H.
  • Journal Title: Microbiology Resource Announcements Volume: 7 Issue: 16
  • DOI: 10.1128/mra.01185-18
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Notch signaling regulates the expression of glycolysis-related genes in a context-dependent manner during embryonic development (2018)
  • Author(s): Kuwabara Shuhei、Yamaki Misato、Yu Huiqing、Itoh Motoyuki
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 503 Issue: 2 Pages: 803-808
  • DOI: 10.1016/j.bbrc.2018.06.079
  • Peer Reviewed
• [Journal Article] Expression levels of UL16 binding protein 1 and natural killer group 2 member D affect overall survival in patients with gastric cancer following gastrectomy (2018)
  • Author(s): Kamei R, Yoshimura K, Yoshino S, Inoue M, Asao T, Fuse M, Wada S, Kuramasu A, Furuya-Kondo T, Oga A, Iizuka N, Suzuki N, Maeda N, Watanabe Y, Matsukuma S, Iida M, Takeda S, Ueno T, Yamamoto N, Fukagawa T, Katai H, Sasaki H, Hazama S, Oka M, Nagano H.
  • Journal Title: Oncology Letters Volume: 15 Pages: 747-754
  • DOI: 10.3892/ol.2017.7354
  • Peer Reviewed / Open Access
• [Journal Article] Multiple roles of single-minded 2 in esophageal squamous cell carcinoma and its clinical implications (2018)
  • Author(s): Tamaoki M, Komatsuzaki R, Komatsu M, Minashi K, Aoyagi K, Nishimura T, Chiwaki F, Hiroki T, Daiko H, Morishita K, Sakai Y, Seno H, Chiba T, Muto M, Yoshida T, Sasaki H
  • Journal Title: Cancer Sci. Volume: 109 Issue: 4 Pages: 1121-1134
  • DOI: 10.1111/cas.13531
  • NAID: 120006457186
  • Peer Reviewed / Open Access
• [Journal Article] Chromatin immunoprecipitation and DNA sequencing identified a LIMS1/ILK pathway regulated by LMO1 in neuroblastoma (2018)
  • Author(s): Saeki N., Saito A., Sugaya Y., Amemiya M., Ono H., Komatsuzaki R., Yanagihara K., Sasaki H.
  • Journal Title: Cancer Genomics Proteomics Volume: 15 Issue: 3 Pages: 165-174
  • DOI: 10.21873/cgp.20074
  • Peer Reviewed / Open Access
• [Journal Article] PTCH1 pathway network model in diffuse-type gastric cancer and epithelial-mesenchymal transition (2018)
  • Author(s): Tanabe S., Aoyagi K., Yokozaki H., Sasaki H.
  • Journal Title: J. Stem Cell Res. Med. Volume: 3 Issue: 1 Pages: 1-15
  • DOI: 10.15761/jscrm.1000128
  • Peer Reviewed / Open Access
• [Journal Article] Molecular network pathway of ERBB in diffuse-type gastric cancer, mesenchymal stem cells and epithelial-mesenchymal transition (2018)
  • Author(s): Tanabe S., Aoyagi K., Yokozaki H., Sasaki H.
  • Journal Title: Journal of Clinical Epigenetics Volume: 4 Issue: 02 Pages: 13-13
  • DOI: 10.21767/2472-1158.100098
  • Peer Reviewed / Open Access
• [Journal Article] Indirect Down-regulation of Tumor-suppressive let-7 Family MicroRNAs by LMO1 in Neuroblastoma (2018)
  • Author(s): Saeki N., Saito A., Sugaya Y., Amemiya M., Sasaki H.
  • Journal Title: Cancer Genomics Proteomics Volume: 15 Issue: 5 Pages: 413-420
  • DOI: 10.21873/cgp.20100
  • Peer Reviewed / Open Access
• [Journal Article] Molecular pathway network of EFNA1 in cancer and mesenchymal stem cells (2018)
  • Author(s): Tanabe S., Aoyagi K., Yokozak K., Sasaki H.
  • Journal Title: AIMS Cell and Tissue Engineering Volume: 2 Issue: 2 Pages: 58-77
  • DOI: 10.3934/celltissue.2018.2.58
  • Peer Reviewed / Open Access
• [Journal Article] A genetic link between epigenetic repressor AS1–AS2 and DNA replication factors in establishment of adaxial–abaxial leaf polarity of <i>Arabidopsis</i> (2018)
  • Author(s): Luong Quy Toan, Keta Sumie, Asai Toshiharu, Kojima Shoko, Nakagawa Ayami, Micol Jose, Xia Shitou, Machida Yasunori, Machida Chiyoko
  • Journal Title: Plant Biotechnology Volume: 35 Issue: 1 Pages: 39-49
  • DOI: 10.5511/plantbiotechnology.18.0129b
  • NAID: 130006727681
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Editorial: Novel aspects of plant nucleolar functions in plant growth and development (2018)
  • Author(s): Munetaka Sugiyama, Yasunori Machida
  • Journal Title: Frontiers in Plant Science Volume: 9 Pages: 814-814
  • DOI: 10.3389/fpls.2018.00814
  • Peer Reviewed / Open Access
• [Presentation] ゲノムのジャンク領域における機能領域予測とその実験的検証 (2020)
  • Author(s): 本村汰一朗・宮木至道・林憲哉・尾之内均・伊藤素行・髙橋広夫
  • Organizer: 第48回東海ファジィ研究会、日間賀島公民館、南知多町
  • Year and Date: 2020-02-16
• [Presentation] ゲノムのジャンク領域における機能領域予測とその実験的検証 (2020)
  • Author(s): 本村汰一朗・宮木至道・林憲哉・尾之内均・伊藤素行・高橋広夫
  • Organizer: 第48回東海ファジィ研究会
• [Presentation] 植物間で保存された翻訳調節に関与する非AUG型uORFのゲノムワイド探索 (2020)
  • Author(s): 平郡雄太・高橋広夫・林憲哉・佐々木駿・山下由衣・内藤哲・尾之内均
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] ヒトゲノムにおける翻訳制御上流ORFのデータベース横断解析による推定と実験的検証 (2020)
  • Author(s): 本村汰一朗, 宮木 至道, 伊藤 素行, 尾之内 均, 福吉修一, 高橋 広夫
  • Organizer: 日本遺伝子学会第92回大会
• [Presentation] 過剰なステロール蓄積が植物の生理機能に与える影響 (2020)
  • Author(s): 島田貴士・重信秀治・山口勝司・高橋広夫・福吉修一・上田貴志・西村いくこ
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] シロイヌナズナのAS2が関わる葉の向背軸分化とAS2の標的遺伝子のDNAメチル化における核小体タンパク質の役割の解明 (2020)
  • Author(s): 町田千代子・日比野哲紀・三谷恭平・水谷優斗・ヴィアルプラデルシモン・高橋広夫・小島晶子・町田泰則
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] 上流ORFの新生ペプチドを含む翻訳複合体が細胞内のマグネシウム濃度を感知して翻訳を制御する (2020)
  • Author(s): 林憲哉・佐々木駿・平郡雄太・Feng Zhihang・藤原徹・渡部敏裕・町田幸大・今高寛晃・高橋広夫・山下由衣・内藤哲・尾之内均
  • Organizer: 第61回日本植物生理学会年会
• [Presentation] The Wnt/beta-catenin signaling pathway and its relation to cancer and stem cells、 (2020)
  • Author(s): 田邊思帆里、青柳一彦、Sabina Quader、小野竜一、広瀬明彦、横崎 宏、佐々木博己
  • Organizer: 第2回医薬品毒性機序研究会
• [Presentation] 骨髄由来間葉系幹細胞及び胃がんにおける上皮間葉転換関連分子パスウェイ及びがん幹細胞ネットワーク (2020)
  • Author(s): 田邊思帆里、青柳一彦、カーデル サビーナ、カブラル オラシオ、小野竜一、広瀬明彦、横崎 宏、佐々木博己
  • Organizer: 第19回日本再生医療学会総会「Web」
• [Presentation] がん関連 Wnt/beta-cateninシグナルパスウェイに関する有害性発現経路(Adverse Outcome Pathway ; AOP)の開発 (2020)
  • Author(s): 田邊思帆里、青柳一彦、Sabina Quader、Horacio Cabral、小野竜一、広瀬明彦、横崎 宏、佐々木博己
  • Organizer: 第93回日本薬理学会年会「Web」
• [Presentation] がん及び幹細胞における Wnt/beta-catenin シグナルパスウェイ (2020)
  • Author(s): 田邊思帆里、青柳一彦、Sabina Quader、Horacio Cabral、小野竜一、広瀬明彦、横崎 宏、佐々木博己
  • Organizer: 第140回日本薬学会年会「Web」
• [Presentation] CANCER STEM CELL AND EPITHELIAL-MESENCHYMAL TRANSITION-RELATED MOLECULAR NETWORK PROFILING IN MESENCHYMAL STEM CELLS AND GASTRIC CANCER (2020)
  • Author(s): Shihori Tanabe, Sabina Quader, Ryuichi Ono, Horacio Cabral, Kazuhiko Aoyagi, Akihiko Hirose, Hiroshi Yokozaki, Hiroki Sasaki
  • Organizer: ISSCR (International Society for Stem Cell Research) 2020 Annual Meeting, Virtual 「Web」
  • Int'l Joint Research
• [Presentation] びまん型胃がん及び腸型胃がんにおけるネットワーク解析による Wnt/beta-cateninシグナルパスウェイ関連 Adverse Outcome Pathway (AOP)の開発 (2020)
  • Author(s): 田邊思帆里、Sabina Quader、小野竜一、Horacio Cabral、青柳一彦、広瀬明彦、横崎 宏、佐々木博己
  • Organizer: 第10回レギュラトリーサイエンス学会学術大会
• [Presentation] 抗がん剤開発におけるPDXモデルの日本での利用状況 (2020)
  • Author(s): 古賀宣勝、津村陵、濱田哲暢、桑田健、佐々木博己、土井俊彦、合川勝二、大橋紹宏、片野いくみ、五十嵐美徳、伊藤守、落合淳志
  • Organizer: 第79回日本癌学会学術総会「Web」
• [Presentation] ヒト患者由来腹膜転移癌に対する抗がん剤の増殖抑制作用の検討 (2020)
  • Author(s): 森川崇、柿田実里、本堀佳納、麓美里、原田恵子、榎成憲、片山誠一、松崎圭佑、小松将之、竹下文隆、千脇史子、佐々木博己
  • Organizer: 第79回日本癌学会学術総会「Web」
• [Presentation] びまん型及び腸型胃がんにおけるRNAウィルス感染分子ネットワーク制御機構の解明 (2020)
  • Author(s): 田邊思帆里、Sabina Quader、小野竜一、Horacio Cabral、青柳一彦、広瀬明彦、横崎 宏、佐々木博己
  • Organizer: 第79回日本癌学会学術総会「Web」
• [Presentation] びまん型及び腸型胃がんにおけるRNAウィルス感染分子ネットワーク制御機構の解明 (2020)
  • Author(s): 田邊思帆里、カデール サビーナ、小野竜一、カブラル オラシオ、青柳一彦、広瀬明彦、横崎 宏、佐々木博己
  • Organizer: 第6回次世代を担う若手のためのレギュラトリーサイエンスフォーラム「Web」
• [Presentation] 分子ネットワークマッピング解析を用いたバイオ疾患プロファイリングによる治療薬ターゲット探索 (2020)
  • Author(s): 田邊思帆里、カデール サビーナ、小野竜一、カブラル オラシオ、青柳一彦、広瀬明彦、横崎 宏、佐々木博己
  • Organizer: 第43回日本分子生物学会年会「Web」
• [Presentation] 神経発生期と脳疾患におけるNotchシグナル機能と制御 (2020)
  • Author(s): 伊藤素行
  • Organizer: 日本薬学会第140年会
• [Presentation] 遺伝性難病CADASILにおける変異NOTCH3凝集メカニズムの解析 (2020)
  • Author(s): 鈴木翔大・松本岳海・日浦智史・伊藤素行
  • Organizer: 日本薬学会第140年会
• [Presentation] 加齢に伴うigf2bp3とIGFシグナル経路分子の発現相関の解析 (2020)
  • Author(s): 今井萌乃・李穎怡・伊藤素行
  • Organizer: 日本薬学会第140年会
• [Presentation] グリオーマにおけるNotchシグナルのHK2発現調節機構の解明 (2020)
  • Author(s): 桑原周平・八巻実里・兪慧晴・伊藤素行
  • Organizer: 日本薬学会第140年会
• [Presentation] Development of bioinformatics methods and their applications : from plant to medical sciences (2019)
  • Author(s): Takahashi H., Machida C., Muto M., Kojima S., Itoh M., Onouchi H., and Sasaki H.
  • Organizer: International Conference for Leading and Young Medical Scientists - IC-LYMS 2019-
  • Int'l Joint Research / Invited
• [Presentation] Roles of Nucleolar Proteins and Zinc-Finger Protein ASYMMETRIC LEAVES2 in establishment of leaf adaxial-abaxial polarity in Arabidopsis thaliana (2019)
  • Author(s): Machida C., Vial-Pradel S., Nomoto M., Takahashi H., Kojima S., Tada Y and Machida Y.
  • Organizer: Plant Organ Growth Symposium
  • Int'l Joint Research
• [Presentation] Epigenetic role of zinc-finger protein AS2 and nucleolar proteins in the specification of the adaxial cell fate of leaves in Arabidopsis thaliana (2019)
  • Author(s): Machida C., Ando S., Sakamoto Y., Matsunaga S., Kurihara D., Higashiyama T., Takahashi H., Kojima S., Machida Y.
  • Organizer: 第42回日本分子生物学会年会
  • Invited
• [Presentation] 翻訳制御上流ORFのバイオインフォマティクスによる推定と実験的検証 (2019)
  • Author(s): 宮木至道・林憲哉・尾之内均・伊藤素行・高橋広夫
  • Organizer: 日本遺伝学会第91回大会
• [Presentation] 葉にオイルボディを過剰蓄積するシロイヌナズナ変異体lipid rich 1の解析 (2019)
  • Author(s): 山口萌・重信秀治・山口勝司・高橋広夫・福吉修一・島田貴士
  • Organizer: 第32回植物脂質シンポジウム
• [Presentation] 植物ステロール過剰蓄積が及ぼす植物体への影響 (2019)
  • Author(s): 島田貴士・重信秀治・山口勝司・高橋広夫・福吉修一・上田貴志・西村いくこ
  • Organizer: 第32回植物脂質シンポジウム
• [Presentation] 植物間で保存された翻訳調節に関与する非AUG型uORFのゲノムワイド探索 (2019)
  • Author(s): 平郡雄太・高橋広夫・林憲哉・佐々木駿・山下由衣・内藤哲・尾之内均
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] NCBNにおけるがん研究とゲノム医療のためのバイオリソース (2019)
  • Author(s): 吉田輝彦・濱田哲暢・佐々木博己・間野博行
  • Organizer: 第78回日本癌学会学術総会
• [Presentation] カゼインキナーゼ2の阻害は、インスリン様増殖因子2受容体の調整不全を介してライソソーム機能不全を導く (2019)
  • Author(s): 竹田貴・小松将之・千脇史子・小松崎理絵・小林佑介・冨永英一郎・阪埜浩司・青木大輔・佐々木博己
  • Organizer: 第78回日本癌学会学術総会
• [Presentation] 胃がん及び幹細胞における上皮間葉転換関連ネットワークパスウェイ (2019)
  • Author(s): 田邊思帆里・カーデルサビーナ・小野竜一・青柳一彦・広瀬明彦・横崎宏・佐々木博己
  • Organizer: 第78回日本癌学会学術総会
• [Presentation] Development of bioinformatics methods and their applications : from plant to medical sciences (2019)
  • Author(s): Takahashi H., Machida C., Muto M., Kojima S., Itoh M., Onouchi H., and Sasaki H
  • Organizer: International Conference for Leading and Young Medical Scientists - IC-LYMS 2019-
• [Presentation] 上流ORFの新生ペプチドを含む翻訳複合体が細胞内のマグネシウム濃度を感知して翻訳を制御する (2019)
  • Author(s): 林憲哉・佐々木駿・平郡雄太・Feng Zhihang・藤原徹・高橋広夫・山下由衣・内藤哲・尾之内均
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] シロイヌナズナのAS2を介した葉の発生に関わるサイトカイニン合成酵素遺伝子の解析 (2019)
  • Author(s): 小島晶子・西本珠美・香田加那・石橋菜々子・小嶋美紀子・高橋広夫・榊原均・町田泰則・町田千代子
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] 選択的スプライシングと上流ORFによる翻訳制御を介した植物の核小体ストレス応答機構 (2019)
  • Author(s): 佐々木駿・工藤凛・笹原大暉・高橋広夫・渡部俊・大林祝・杉山宗隆・内藤哲・尾之内均
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] シロイヌナズナのzinc-finger-like protein AS2が関わる葉の向背軸分化とDNA メチル化における核小体タンパク質の役割の解明 (2019)
  • Author(s): 鈴木雅貴・ヴィアルプラデルシモン・高橋広夫・杉山宗隆・氣多澄江・小島晶子・町田泰則・町田千代子
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Development of bioinformatics methods and their applications: from plant to medical sciences (2019)
  • Author(s): Takahashi H., Machida C., Muto M., Kojima S., Itoh M., Onouchi H., and Sasaki H.
  • Organizer: International Conference for Leading and Young Medical Scientists - IC-LYMS 2019 -
  • Int'l Joint Research / Invited
• [Presentation] カゼインキナーゼ2の阻害は、インスリン様増殖因子2 受容体の調整不全を介してライソソーム機能不全を導く (2019)
  • Author(s): 竹田貴・小松将之・千脇史子・小松崎理絵・小林佑介・冨永英一郎・阪埜浩司・青木大輔・佐々木博己
  • Organizer: 第78回日本癌学会学術総会
• [Presentation] A new live staining probe, FITC-BPC3: for zebrafish fibrous tissues (2019)
  • Author(s): Wang Z., Kurihara T., Nakajima S., Mizoguchi T., Nemoto T. and Itoh M.
  • Organizer: 第25回小型魚類研究会
• [Presentation] 糖尿病誘導ゼブラフィッシュにおける鱗を利用した骨病態の評価 (2019)
  • Author(s): 兪慧晴・佐藤巧都・Siripat Chaishit・田中佑樹・小椋康光・伊藤素行
  • Organizer: 第５回創薬ゼブラフィッシュメダカ研究会
• [Presentation] ゼブラフィッシュ成魚を用いた脳梗塞モデルの確立と脳機能回復過程の解析 (2019)
  • Author(s): 大北真由・溝口貴正・伊藤素行
  • Organizer: 第５回創薬ゼブラフィッシュメダカ研究会
• [Presentation] A new live staining probe, FITC-BPC3: for zebrafish fibrous tissues (2019)
  • Author(s): Wang Z., Kurihara T., Nakajima S., Mizoguchi T., Nemoto T. and Itoh M.
  • Organizer: 第５回創薬ゼブラフィッシュメダカ研究会
• [Presentation] Zebrafish behavior pattern recognition using three-dimensional tracking and machine learning (2018)
  • Author(s): Yang P., Kajiwara R., Takahashi H., Itoh M.
  • Organizer: The 24th Japanese medaka and zebrafish meet
• [Presentation] Arabidopsis zinc-finger-like protein ASYMMETRIC LEAVES2 (AS2) bound exon 1 of ETTIN (ARF3) and maintain gene body DNA methylation in ETTIN together with nucleolar proteins (2018)
  • Author(s): Vial-Pradel S., Nomoto M., Takahashi H., Ando S., Suzuki M., Kojima S., Tada Y., Machida Y., Machida C.
  • Organizer: 日本植物学会第82回大会
• [Presentation] がんオミックスデータに基づく治療前診断・治療標的探索 (2018)
  • Author(s): 高橋広夫・武藤学・佐々木博己
  • Organizer: 化学工学会 第50回秋季大会
• [Presentation] シロイヌナズナの葉の極性確立においてAS1-AS2とTOP1αはETTIN (ARF3)を介してCDKインヒビターを抑制する (2018)
  • Author(s): 町田千代子・中川彩美・高橋広夫・ビアループラデル シモン・山川 美里・小島晶子・町田泰則
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] Arabidopsis zinc-finger-like protein ASYMMETRIC LEAVES2 (AS2) bound exon 1 of ETTIN (ARF3) and maintain gene body DNA methylation in ETTIN together with nucleolar proteins (2018)
  • Author(s): Vial Pradel S., Nomoto M., Takahashi H., Ando S., Suzuki M., Kojima S., Tada Y., Machida Y., Machida C.
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] Arabidopsis zinc-finger-like protein ASYMMETRIC LEAVES2 (AS2) binds exon 1 of ETTIN(ARF3) and maintains gene body DNA methylation in ETTIN together with nucleolar proteins. (2018)
  • Author(s): Vial-Pradel S., Nomoto M., Takahashi H., Ando S., Suzuki M., Kojima S., Tada Y., Machida Y., Machida C.
  • Organizer: EMBO Workshop "New shores in land plant"
  • Int'l Joint Research
• [Presentation] Bioinformatic identification method of evolutional ranges for functional small peptides on genomes (2018)
  • Author(s): Takahashi H., Hayashi N., Takahashi A., Onouchi H.
  • Organizer: The 2nd edition of Biotech France 2018 International Conference and Exhibition -Biotech France 2018-
  • Int'l Joint Research
• [Presentation] Bioinformatic identification of evolutionary ranges for functional small peptides in genomes (2018)
  • Author(s): Takahashi H., Hayashi N., and Onouchi H.
  • Organizer: International Symposium on "Proteins; from the Cradle to the Grave"
  • Int'l Joint Research
• [Presentation] 多層オミックス情報のある腹膜再発がん患者腹水から樹立された124種のPDC（Patient-derived cell line）とその高い有用性 (2018)
  • Author(s): 佐々木博己
  • Organizer: 【新しい治療法の開発を目指す患者由来がんモデル】講演会
  • Invited
• [Presentation] Combination of network pathway and epithelial-mesenchymal transition-related gene expression in gastric cancer and mesenchymal stem cells (2018)
  • Author(s): Tanabe S., Aoyagi K., Yokozaki H., Sasaki H.
  • Organizer: The 18th World Congress of Basic and Clinical Pharmacology (WCP2018)
  • Int'l Joint Research
• [Presentation] Network pathways of PTCH1, ERBB3, CTNNB1 and EFNA1 in gastric cancer and mesenchymal stem cells (2018)
  • Author(s): Tanabe S., Aoyagi K., Yokozaki H., Sasaki H.
  • Organizer: 5th Tissue Engineering and Regenerative Medicine International Society World Congress - 2018 Kyoto
  • Int'l Joint Research
• [Presentation] Knockdown of SIM2 enhances radio-resistance and tumor growth by inducing HIF1A in cervical squamous cell carcinoma (2018)
  • Author(s): Chiwaki F., Kobayashi Y., Banno K., Iijima M., Takeda T., Tominaga E., Sasaki H., Aoki D.
  • Organizer: 17th International Gynecologic Cancer Society Meeting (IGCS)
  • Int'l Joint Research
• [Presentation] オーロラキナーゼの遮断はがん遺伝子中毒を新たに誘導することでEGFR阻害剤への感受性を亢進させる (2018)
  • Author(s): 小松将之・中村加奈子・千脇史子・竹田貴・小松崎理絵・阪埜浩司・青木大輔・佐々木博己
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] 食道扁平上皮がんにおけるパルボシクリブとエルロチニブの併用療法の非臨床試験 (2018)
  • Author(s): 小松崎理絵・小松将之・千脇史子・三谷幸代・河野隆志・市川仁・佐々木博己
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] 患者腹水中がん細胞の徹底的な特性評価 (2018)
  • Author(s): 千脇史子・坂本裕美・小松将之・松下弘道・小松崎理絵・市川仁・濱口哲弥・朴成和・平岡伸介・松崎圭祐・落合淳志・吉田輝彦・佐々木博己
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] IGF2Rは、子宮頸癌の予後不良なバイオマーカーであり、癌細胞の生存に寄与する (2018)
  • Author(s): 竹田貴・小松将之・千脇史子・小松崎理絵・小林佑介・富永英一郎・阪埜浩司・青木大輔・佐々木博己
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] 肺腺がん由来PDX/CDXモデルにおけるがん－間質相互作用による発現遺伝子の変化 (2018)
  • Author(s): 石ケ守里加子・高橋真美・河野隆志・佐々木博己・今井俊夫
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] 神経芽腫において、がん促進転写調節因子LMO1はがん抑制的let-7ファミリーmicroRNAの発現を抑制している (2018)
  • Author(s): 佐伯宣久・斎藤聡・菅谷勇樹・雨宮光宏・佐々木博己
  • Organizer: 第41回日本分子生物学会
• [Presentation] Notch signaling regulates expression of glycolytic genes during development (2018)
  • Author(s): Yamaki M., Kuwabara S., Yu H., Itoh M.
  • Organizer: Joint Annual Meeting of 70th JSCB and 51st JSDB
• [Presentation] Precise regulation of neuron-specific Notch signal is required for neuronal differentiation and locomotive behavior (2018)
  • Author(s): Fukagawa S., Mizoguchi T., Iihama M., Fukada M., Song X., Itoh M.
  • Organizer: Joint Annual Meeting of 70th JSCB and 51st JSDB
• [Presentation] Identification of neuropeptides regulating memory formation (2018)
  • Author(s): Sizhe R., Nakashima H., Itoh M., Tonoki A.
  • Organizer: The 11th Workshop for Molecular Analysis of Higher Brain Function
  • Int'l Joint Research
• [Presentation] Transient Notch signal activation is required for V2a/V2b interneuron cell fate choice, and normal V2b differentiation and morphology (2018)
  • Author(s): Mizoguchi T., Iihama M., Fukagawa S., Song X., Nakaura M., Fukada M., Itoh M.
  • Organizer: The 24th Japanese medaka and zebrafish meeting
• [Presentation] Glycolysis-related gene expression is context dependently regulated by Notch signaling during zebrafish neural development (2018)
  • Author(s): Kuwabara S., Yamaki M., Yu H., Itoh M.
  • Organizer: The 24th Japanese medaka and zebrafish meeting
• [Presentation] Zebrafish behavior pattern recognition using three-dimensional tracking and machine learning (2018)
  • Author(s): Yang P., Kajiwara R., Takahashi H., Itoh M.
  • Organizer: The 24th Japanese medaka and zebrafish meeting
• [Presentation] Notchリガンドビーズを用いたNotchリガンド/受容体EGF様リピートと糖鎖修飾の機能解析 (2018)
  • Author(s): 大丸修平・劉楽笛・伊藤素行
  • Organizer: 第17回 次世代を担う若手ファーマ・バイオフォーラム
• [Presentation] 過食と恒明条件飼育によるゼブラフィッシュの糖尿病様病態の誘導 (2018)
  • Author(s): 梶原理希・楊鵬・桑原周平・伊藤素行
  • Organizer: 第17回 次世代を担う若手ファーマ・バイオフォーラム
• [Presentation] NO-sGC pathway potentially contributes to age-related memory impairment in Drosophila (2018)
  • Author(s): Nagai S., Yu Z., Takahashi H., Itoh M., Tonoki A.
  • Organizer: The 13th Japanese *Drosophila* Research Conference
• [Presentation] ゼブラフィッシュ胚背側化誘導化合物のBMPシグナル抑制メカニズム解析 (2018)
  • Author(s): 近藤優衣・矢藤まり・三上翔平・大倉航・溝口貴正・野田翔太・天野剛志・廣明秀一・伊藤素行
  • Organizer: 第62回日本薬学会関東支部大会
• [Presentation] 自動給餌装置と明暗コントロールによるゼブラフィッシュの空腹時高血糖の誘導 (2018)
  • Author(s): 梶原理希・楊鵬・桑原周平・伊藤素行
  • Organizer: 第4回ゼブラフィッシュ・メダカ創薬研究会
• [Presentation] DeltaA dependent Notch signal is required for effective escape behavior in zebrafish (2018)
  • Author(s): Mizoguchi T., Ji S., Chin M., Itoh M.
  • Organizer: 第41回日本分子生物学会年会
• [Book] 患者由来がんモデルを用いたがん研究実践ガイド (2019)
  • Author(s): 編集・著者 佐々木博己
  • Total Pages: 293
  • Publisher: 羊土社
• [Remarks] 高橋広夫研究室
  • URL: https://www.takahashi-lab.com/