| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
|
|---|
| Outline of Final Research Achievements |
DNA molecules that carry the genetic information of the cell are constantly associated with various proteins. DNA-associated proteins are covalently trapped in DNA to form DNA-protein cross-links (DPCs) when cells are irradiated with ionizing radiation or treated with other DNA-damaging agents. However, little is known about the amount of DPC damage resulting from the exposure to ionizing radiation and other DNA-damaging agents. Furthermore, how DPC damage is repaired in cells remains largely elusive. In the present study, we irradiated cells with X-rays and analyzed the proteins involved in DPCs. The possible mechanisms of DPC formation are discussed. We also used cells that were deficient in tyrosyl-DNA phosphodiesterases (TDP) 1 and 2 together with other enzymes, and elucidated the roles of these enzymes in the repair of DPCs that contained the cleavage complex of topoisomerases 1 and 2 (TOP1cc and TOP2cc).
|
