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Combined effects of high seawater temperature and anthropogenic chemicals on coral bleaching using advanced molecular biological analysis

Research Project

Project/Area Number 18H03387
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 63040:Environmental impact assessment-related
Research InstitutionEhime University

Principal Investigator

Takeuchi Ichiro  愛媛大学, 農学研究科, 教授 (30212020)

Co-Investigator(Kenkyū-buntansha) 高山 弘太郎  愛媛大学, 農学研究科, 教授 (40380266)
山城 秀之  琉球大学, 熱帯生物圏研究センター, 教授 (80341676)
石橋 弘志  愛媛大学, 農学研究科, 准教授 (90403857)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywordsサンゴ / 高水温 / 環境化学物質 / 毒性影響評価 / 分子生物学的解析 / 毒性評価
Outline of Final Research Achievements

The genus Acropora comprises one of the major groups of hermatypic corals along the southern coast of Japan. Irgarol was originally formulated as an herbicide and is now also used in antifouling paints for ship hulls. Toxicity experiments were conducted to evaluate the effects of Irgarol 1051 and high seawater temperatures on Acropora spp. Acropora tenuis, a representative species in southern Japan, was bleached at 32 °C, but not at 30 °C. The combined effects of high sea water temperature (32 °C) and herbicides were confirmed on the photosynthetic efficiency of zooxanthellae, symbiotic algae within the coral, and on the expression of various genes, including those encoding heat shock protein, of both corals and symbiotic algae. Th results suggest that both corals and symbiotic zooxanthellae are susceptible to the effects of Irgarol at a seawater temperature of 32 °C.

Academic Significance and Societal Importance of the Research Achievements

サンゴ礁生態系は地球上で最も生物多様性及び生産性が高い生態系であるが、近年、世界各地で大規模なサンゴ白化が発生している。サンゴの白化は、高水温だけでなく、赤土、富栄養化や人工化学物質等も関与していることが指摘されている。本研究により、32℃の高水温下では、近年、船底防汚塗料としても使用されているイルガロールが、ミドリイシ属サンゴへ複合影響を及ぼすことが明らかになった。よって、除草剤等の人工化学物質の影響を低減させると、高水温の影響が緩和される可能性が示唆され、気候変動下でのサンゴ礁生態系の保全政策立案に貢献すると考えられた。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (12 results)

All 2021 2020 2019 2018

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (7 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Thermal tolerance of the hermatypic coral Acropora tenuis elucidated by RGB analysis and expression of heat shock proteins in coral and symbiotic dinoflagellates.2021

    • Author(s)
      Shitaoka, R., Ishibashi, H. and Takeuchi, I.
    • Journal Title

      Marine Pollution Bulletin

      Volume: 162 Pages: 111812-111812

    • DOI

      10.1016/j.marpolbul.2020.111812

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of the herbicide Irgarol 1051 on the transcriptome of hermatypic coral Acropora tenuis and its symbiotic dinoflagellates.2021

    • Author(s)
      Ishibashi, H., Takaichi, D. and Takeuchi, I.
    • Journal Title

      Science of the Total Environment

      Volume: 780 Pages: 146542-146542

    • DOI

      10.1016/j.scitotenv.2021.146542

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Succession of delayed fluorescence correlated with coral bleaching in the hermatypic coral acropora tenuis.2020

    • Author(s)
      Takeuchi, I., Takaichi, D., Katsumata, M. and Ishibashi, H.
    • Journal Title

      Marine Pollution Bulletin

      Volume: 154 Pages: 111008-111008

    • DOI

      10.1016/j.marpolbul.2020.111008

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of ecologically relevant concentrations of Irgarol 1051 in tropical to subtropical coastal seawater on hermatypic coral Acropora tenuis and its symbiotic dinoflagellates.2020

    • Author(s)
      Kamei, M., Takayama, K., Ishibashi, H. and Takeuchi, I.
    • Journal Title

      Marine Pollution Bulletin

      Volume: 150 Pages: 110734-110734

    • DOI

      10.1016/j.marpolbul.2019.110734

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Usage of UV-curable oligomer-based adhesive agent in hermatypic coral experimental research.2019

    • Author(s)
      Takeuchi, I., Yamashiro, H. and Gushi, M.
    • Journal Title

      MethodsX

      Volume: 6 Pages: 1600-1607

    • DOI

      10.1016/j.mex.2019.06.007

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Effects of high temperature and a PSII herbicide (Irgarol 1051) on hermatypic coral Acropora tenuis evaluated using a compact ecotoxicity experiment system2020

    • Author(s)
      Gushi, M., Takayama, K., Ishibashi, Yamashiro, Takeuchi, I.
    • Organizer
      SETAC SciCon. SETAC Europe 30th Annual Meeting.
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 高水温下でIrgarol 1051(除草剤)に曝露したウスエダミドリイシAcropora tenuisの体色と光合成収率の変動過程の解析2020

    • Author(s)
      具志美香子・高山弘太郎・石橋弘志・竹内一郎
    • Organizer
      2020年日本プランクトン学会・日本ベントス学会合同大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 30~32℃の高水温に曝露したコユビミドリイシAcropora digitiferaの体色と光合成収率の変動過程の解析2020

    • Author(s)
      水口雅貴・石橋弘志・高山弘太郎・山城秀之・竹内一郎
    • Organizer
      日本サンゴ礁学会第23回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] The mechanism underlying Acropora tenuis bleaching by a photosystem ii herbicide, elucidated by measuring delayed fluorescence.2019

    • Author(s)
      Takeuchi, I., Takaichi, D., Katsumata, M. and Ishibashi, H.
    • Organizer
      The 9th International Conference on Marine Pollution and Ecotoxicology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 30℃でIrgarol 1051に曝露したウスエダミドリイシAcropora tenuisの体色とクロロフィル蛍光収率の変動過程2019

    • Author(s)
      具志美香子・高山弘太郎・山城秀之・石橋弘志・竹内一郎
    • Organizer
      日本サンゴ礁学会第22回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Transcriptome analysis to assess coral bleaching induced by Irgarol 1051.2018

    • Author(s)
      Ishibashi, H., Takaishi, D., and Takeuchi, I.
    • Organizer
      The Second International Workshop on Eco-shoreline Designs for Sustainable Coastal Development.
    • Related Report
      2018 Annual Research Report
  • [Presentation] Effects of ambient levels of Irgarol 1051 in tropical coastal seawater on the hard coral Acropora tenuis.2018

    • Author(s)
      Takeuchi, I., Kamei, M., Takayama, K., and Ishibashi, H.
    • Organizer
      What's in Our Water WiOW2018 Symposium.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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