| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
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| Outline of Final Research Achievements |
The study aimed to establish the transgenic model plant expressing MerC and other toxic metal transporters isolated from mercury-resistant bacteria and improve the feasibility of phytoremediation for multi-metal polluted environments. First, we analyzed inorganic mercury accumulation of the Arabidopsis plant lines expressing MerC mainly in the plasma membrane of the epidermis or endodermis. Both cell-type-specific expression of MerC improved mercury accumulation ability to the shoots, and the effect was comparable to the 35S-driven ubiquitously expressing plant. We further developed a vector system harboring up to four expression cassettes in a single T-DNA, which can be applied to introduce multiple Mer transporters to the plant by a single transformation.
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