| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
The purpose of this study is to measure mechanical properties of endothelial primary cilia with the diameter of ca. 200 nm and the length of several micron existing on the cell surface and to identify them as a mechanosensor. We fabricated an in-house micro-tensile tester to measure mechanical properties of primary cilia and successfully obtained their Young's modulus. In addition, when calcium experiments were performed on the isolated primary cilia by tensile stimulation, an increase in calcium expression was observed in the primary cilia, indicating that calcium channels existing on the surface of primary cilia could activate the entry of calcium ion.
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