Elucidation of self-organization of myocardium focusing on cell homing and its application for bio-artificial heart engineering
| Project/Area Number |
18H03517
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 90110:Biomedical engineering-related
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| Research Institution | Osaka University |
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Principal Investigator |
LEE Jong-Kook 大阪大学, 医学系研究科, 特任教授(常勤) (60303608)
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| Co-Investigator(Kenkyū-buntansha) |
坂田 泰史 大阪大学, 医学系研究科, 教授 (00397671)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Keywords | 自己組織化 / iPS細胞由来心筋細胞 / ストレスファイバー / YAPシグナル / Duchenne型筋ジストロフィー / 脱細胞化 / 三次元心筋組織構築 / 成熟化 / 三次元組織構築 / 細胞ホーミング / hippoシグナル / 脱細胞化細胞外マトリックス / 臓器様三次元心筋組織構築 / 3次元イメージング / 三次元イメージング / 臓器様３次元心筋組織構築 |
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| Outline of Final Research Achievements |
We have approached the development of new technologies that can be used to create a three-dimensional bioartificial heart with functions and morphology comparable to living organs:
# 1. We have developed a new decellularization technology using surfactants and glycolipid substances; # 2. We reported a method for promoting maturation and self-organization of human iPS cell-derived cardiomyocytes; # 3. We investigated the relationship between stress fibers (actin) and YAP signals. The result suggests that YAP signals also play an important role in self-organization; # 4. We have established a three-dimensional cardiac imaging technique that can be used for organ-like three-dimensional myocardial tissue by applying the tissue- clearing technique (CIBIC-2).
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| Academic Significance and Societal Importance of the Research Achievements |
心臓全体がびまん性広範囲に障害を受け、重篤な機能不全に陥っている心筋症のような病態に対しては、ドナーからの臓器移植を含む治療が行われているが、臓器そのものを再生し、移植する方法の開発が期待されている。近年、脱細胞化細胞外マトリックスを用いて立体臓器を構築する方法が一定の効果を示し、注目が集まるようになったが、生体臓器に匹敵するバイオ人工心臓を構築するには様々な課題を解決する必要があり、細胞が本来あるべき場所に生着し、生着、成長する、自己組織化を理解することが不可欠である。本課題により、三次元心筋組織構築に有用な知見が得られた。
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Geometrical Patterning and Constituent Cell Heterogeneity Facilitate Electrical Conduction Disturbances in a Human Induced Pluripotent Stem Cell-Based Platform: An In vitro Disease Model of Atrial Arrhythmias2019
Author(s)
Nakanishi H, Lee JK, Miwa K, Masuyama K, Yasutake H, Li J, Tomoyama S, Honda Y, Deguchi J, Tsujimoto S, Hidaka K, Miyagawa S, Sawa Y, Komuro I, Sakata Y.
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Journal Title
Frontiers in Physiology
Volume: 10
Pages: 818-818
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] In vitro platform of allogeneic stem cell-derived cardiomyocyte transplantation for cardiac conduction defects.2018
Author(s)
Yoshida A, Lee JK, Tomoyama S, Miwa K, Shirakawa K, Hamanaka S, Yamaguchi T, Nakauchi H, Miyagawa S, Sawa Y, Komuro I, Sakata Y.
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Journal Title
Europace.
Volume: 20(9)
Issue: 9
Pages: 1553-1560
DOI
Related Report
Peer Reviewed
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