| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
Here we investigated the relationship between the three-dimensional geometry and biological function of focal adhesions or cell-substrate adhesions, a membrane-less organelles. We found using atomic force microscopy together with fluorescence microscopy that focal adhesions possess a specific multi-protein structure in which alpha-actinin-1, one of the actin cross-linking proteins, plays a critical role. Finite element structural analysis together with theoretical analysis suggested that, by having the specific structure, mechanical stress that the individual focal adhesions bear is decreased to allow the structure to be mechanically stabilized. Thus, our findings revealed the biomechanical basis regarding how focal adhesions are stably maintained within cells as the anchorage to the underlying substrate.
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