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Muti-step targeted drug delivery to endoplasmic reticulum via retrograde transport systems

Research Project

Project/Area Number 18H03531
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 90120:Biomaterials-related
Research InstitutionKyoto University

Principal Investigator

Yamashita Fumiyoshi  京都大学, 薬学研究科, 教授 (30243041)

Co-Investigator(Kenkyū-buntansha) 樋口 ゆり子  京都大学, 薬学研究科, 准教授 (40402797)
宗 可奈子  京都大学, 薬学研究科, 助教 (50816684)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Keywordsドラッグデリバリーシステム / 細胞内標的指向化 / 糖鎖 / 小胞体 / 逆行性輸送 / ドラッグデリバリー
Outline of Final Research Achievements

Med-size or macromolecular drugs are expected to have high potency and high selectivity for therapeutic targets, but their cell membrane permeability is severely limited. In this study, we developed drug delivery systems that utilizes selective transport mechanisms of intracellular vesicles to transport drugs from outside the cell to targeted intracellular organelles. By modifying nanoparticles composed of lipids or block co-polymers and proteins containing low molecular weight antibodies with carbohydrate ligands such as sialyl Lewis X and hyaluronic acid, the transport to the endoplasmic reticulum and cytoplasm was promoted, thereby enhancing the therapeutic effect of bioactive substances. Because these systems can deliver macromolecules such as low-molecular-weight antibodies effectively, they would be able to expand the range of therapeutic targets and contribute to the development of new drugs.

Academic Significance and Societal Importance of the Research Achievements

細胞表面の形質膜で認識されて内在化された高分子や粒子はリソソーム分解経路に移行するが、本研究で開発した糖鎖リガンド修飾薬物デリバリーシステムは抗体医薬品等を安定に細胞内小器官に輸送する。抗体医薬品は細胞外あるいは細胞表面を標的とするものしか開発できなかった。一方、細胞内情報伝達の多くはタンパク質間相互作用により起こるが、これは低分子薬物では制御できないという問題もある。本システムにより低分子抗体等高分子物質を必要な部位に送達できれば様々な適用が可能となり、新しい医薬品の創製に貢献できると期待される。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (13 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results)

  • [Int'l Joint Research] University of Pennsylvania(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 江蘇大学(中国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Sialyl LewisX mimic-decorated liposomes for anti-angiogenic everolimus delivery to E-selectin expressing endothelial cells2019

    • Author(s)
      Chantarasrivong Chanikarn、Higuchi Yuriko、Tsuda Masahiro、Yamane Yuuki、Hashida Mitsuru、Konishi Miku、Komura Naoko、Ando Hiromune、Yamashita Fumiyoshi
    • Journal Title

      RSC Advances

      Volume: 9 Issue: 36 Pages: 20518-20527

    • DOI

      10.1039/c9ra01943j

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Binding and structure-kinetic relationship analysis of selective TLR4-targeted immunosuppressive self-assembling heparin nanoparticles2018

    • Author(s)
      Babazada Hasan、Yanamoto Shinya、Hashida Mitsuru、Yamashita Fumiyoshi
    • Journal Title

      International Journal of Pharmaceutics

      Volume: 552 Issue: 1-2 Pages: 76-83

    • DOI

      10.1016/j.ijpharm.2018.09.054

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 糖鎖リガンドsLeX mimicと小胞体局在シグナルペプチドKDELを利用した 小胞体へのタンパク質デリバリー2021

    • Author(s)
      内田結、森川知彦、樋口ゆり子、山下富義
    • Organizer
      第30回日本バイオイメージング学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Targeted delivery of proteins and nucleic acids via carbohydrate binding receptors2021

    • Author(s)
      Fumiyoshi Yamashita
    • Organizer
      Joint Symposium on Frontiers of Pharmaceutical Science and International Pharmaceutical Education
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] sLeX mimic修飾抗 MDM2 scFvによるHUVECの血管新生阻害2020

    • Author(s)
      森川知彦、采女紗也、河村奈緒子、安藤弘宗、樋口ゆり子、山下富義
    • Organizer
      日本薬剤学会 第35年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Targeted delivery of drugs, genes, and cells by using specialized ligand-receptor recognition systems2020

    • Author(s)
      Fumiyoshi Yamashita
    • Organizer
      International Conference and Exhibition on Pharmaceutical Sciences and Technology 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Data mining technologies in accelerating ADME screening2018

    • Author(s)
      Fumiyoshi Yamashita
    • Organizer
      The 5th Asian Symposium on Pharmaceutical Science and Technology & the 5th Enlarged Editorial Board Conference of the Asian Journal of Pharmaceutical Sciences
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 薬学系からみた最近のDDSのトピックと将来展望2018

    • Author(s)
      山下富義
    • Organizer
      第34回日本DDS学会学術集会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Bio-distribution characteristics of liposomes bearing structurally simplified sialyl Lewis X2018

    • Author(s)
      Yuuki Yamane,Chanikarn Chantarasrivong, Ryosaku Ohta, Yuriko Higuchi, Mitsuru Hashida, Fumiyoshi Yamashita
    • Organizer
      日本薬物動態学会第33回年会/MDO国際合同学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 構造単純化シアリルルイス X 修飾リポソームの創製と物性・体内動態評価2018

    • Author(s)
      山根悠暉 、Chantarasrivong Chanikarn、太田亮作、樋口ゆり子、橋田 充、山下富義
    • Organizer
      第68回日本薬学会近畿支部総会・大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Application of machine learning techniques to pharmacokinetic analysis2018

    • Author(s)
      Fumiyoshi Yamashita
    • Organizer
      13th France-Japan DDS Symposium
    • Related Report
      2018 Annual Research Report
    • Invited

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Published: 2018-04-23   Modified: 2023-01-30  

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