| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2018: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Outline of Final Research Achievements |
In biomedical applications utilizing nanoparticles, the nanoparticles are often required to translocate across a cell membrane. Using a molecular dynamics simulation, we here found that even under a weak external electric field that was lower than the membrane breakdown intensity a cationic nanoparticle directly translocates across a model cell membrane without membrane disruption. We then reveal its physical mechanism. At the contact interface between the nanoparticle and the cell membrane, the electric potential across the membrane is locally enhanced by superimposing the nanoparticle surface potential on the externally applied potential, resulting in the nanoparticle direct translocation. We also observed the nanoparticle translocation across artificial cell membrane by using a planer lipid bilayer experimental set-up. Our finding implies that by controlling the nanoparticle-induced local enhancement of the membrane potential the cellular delivery of nanoparticles can be realized.
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