Induction of vasculogenesis and angiogenesis in three-dimensional tissues by using protein expressions with mRNA delivery
| Project/Area Number |
18H03537
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 90120:Biomaterials-related
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
Kobayashi Jun 東京女子医科大学, 医学部, 講師 (20385404)
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| Co-Investigator(Kenkyū-buntansha) |
秋山 義勝 東京女子医科大学, 医学部, 講師 (20349640)
増田 信奈子 東京女子医科大学, 医学部, 助教 (30342851)
中山 正道 東京女子医科大学, 医学部, 講師 (00338980)
辰巳 公平 奈良県立医科大学, 医学部, 准教授 (70555432)
山岡 哲二 国立研究開発法人国立循環器病研究センター, 研究所, 部長 (50243126)
長瀬 健一 慶應義塾大学, 薬学部(芝共立), 准教授 (10439838)
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Keywords | 細胞シート / 血管新生 / 脈管形成 / mRNA送達 / 血管内皮前駆細胞 / 血管前駆細胞 |
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| Outline of Final Research Achievements |
In this study, we developed a novel technique to induce angiogenesis in 3D cell sheet tissue and promote angiogenesis to connect with a host blood vessel. By introducing VEGF-encoding messenger RNA (mRNA) into the 3D cell sheet tissue and allowing it to act like a paracline in the vicinity of vascular endothelial cells, it was suggested to induce angiogenesis inside the 3D cell sheet tissue. It was also found that transplantable liver / cardiomyocyte sheets that rapidly secrete VEGF can be prepared by mRNA delivery. Furthermore, by stacking these, it can be expected to be applied to the construction of a functional three-dimensional tissue having a capillary-like structure of endothelial cells.
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| Academic Significance and Societal Importance of the Research Achievements |
mRNAを利用した脈管形成と同様の内容はこれまでに報告されていない。また、移植可能な3D組織の構築が実現されれば、これまでに不可能であった肝組織の効率的な移植方法につながり、組織再生治療分野における大きなブレークスルーとなると同時に、肝組織再生治療の臨床応用への展開が期待される。
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Report
(5 results)
Research Products
(17 results)
