The Fate of Frozen Cells: Development of Quality Assessment Technology for Therapeutic Cells
| Project/Area Number |
18H03556
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 90140:Medical technology assessment-related
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| Research Institution | National Center for Child Health and Development |
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Principal Investigator |
MIYAMOTO Yoshitaka 国立研究開発法人国立成育医療研究センター, 細胞医療研究部, 研究員 (20425705)
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| Co-Investigator(Kenkyū-buntansha) |
河野 菜摘子 明治大学, 農学部, 専任准教授 (00451691)
中林 一彦 国立研究開発法人国立成育医療研究センター, 周産期病態研究部, 室長 (10415557)
池内 真志 東京大学, 大学院情報理工学系研究科, 講師 (90377820)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | 凍結細胞 / バイオインフォマティクス / 品質評価 / 医療技術 / 再生・細胞医療 |
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| Outline of Final Research Achievements |
The purpose of this research is to "clarify the fate and intracellular potential of frozen cells" and "construct quality evaluation technology for therapeutic cells". Specifically, we conducted "cellular characteristics," "safety," "comprehensive analysis by metabolomics and epigenetics," and "quality judgment and prediction from cell and tissue images" of thawed cells and tissues, and were able to construct an effective quality evaluation technology for use in the fields of regenerative medicine, transplantation medicine, and assisted reproduction. In addition, hierarchical cluster analysis by metabolomics allows us to propose new candidate molecules (evaluation indicators) for quality assurance of frozen cells. By combining various evaluation methods in addition to conventional cell characteristics, it is possible to obtain more accurate information about the state of cells and tissues.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の成果は、医療用細胞の品質を向上させ、細胞医薬品としての安全性、再生・細胞医療における質の改善につながる研究開発である。さらに、光干渉断層撮影（OCT）や機械学習を取り入れることで、非侵襲的に医療用細胞の品質が判定でき、予測することができる。学術的意義として、凍結融解における細胞・組織の特性、メタボロミクスやエピジェネティックスによる網羅的解析から、凍結融解に関与する候補分子を推定し、そのメカニズムを解明することで、細胞機能の改善につなげることができる。社会的意義として、細胞・組織を凍結することでいつでも供給することが可能となり、再生医療、移植医療や生殖補助医療などへの貢献が期待できる。
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Report
(4 results)
Research Products
(72 results)
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[Journal Article] Suppression of Non-Random Fertilization by MHC Class I Antigens2020
Author(s)
Kamiya Junki、Kang Woojin、Yoshida Keiichi、Takagi Ryota、Kanai Seiya、Hanai Maito、Nakamura Akihiro、Yamada Mitsutoshi、Miyamoto Yoshitaka、Miyado Mami、Kuroki Yoko、Hayashi Yoshiki、Umezawa Akihiro、Kawano Natsuko、Miyado Kenji
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Journal Title
International Journal of Molecular Sciences
Volume: 21
Issue: 22
Pages: 8731-8731
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Membrane protein CD9 is repositioned and released to enhance uterine function.2019
Author(s)
Iwai M, Hamatani T, Nakamura A, Kawano N, Kanai S, Kang W, Yoshii N, Odawara Y, Yamada M, Miyamoto Y, Saito T, Saito H, Miyado M, Umezawa A, Miyado K, Tanaka M.
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Journal Title
Laboratory Investigation
Volume: 99
Issue: 2
Pages: 200-209
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Degradation of phosphate polymer polyP enhances lactic fermentation in mice.2018
Author(s)
Nakamura A, Kawano N, Motomura K, Kuroda A, Sekiguchi K, Miyado M, Woojin K, Miyamoto Y, Hanai M, Iwai M, Yamada M, Hamatani T, Saito T, Saito H, Tanaka M, Umezawa A, Miyado K.
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Journal Title
Genes Cells.
Volume: 23(10)
Issue: 10
Pages: 904-914
DOI
Related Report
Peer Reviewed / Open Access
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