| Budget Amount *help |
¥44,070,000 (Direct Cost: ¥33,900,000、Indirect Cost: ¥10,170,000)
Fiscal Year 2021: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2020: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2019: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2018: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
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| Outline of Final Research Achievements |
We constructed a sensor system that can measure cell contraction using a MEMS force sensor and measured the beating of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). This sensor system is equipped with a function to stimulate cells to stretch, and the sensor signal can be used for feedback control of the amount of stretching. Experimental results showed that the greater the amount of cell extension, the stronger the beating force. This means that the Frank-Starling law is valid at the cellular level. Using feedback control, we were able to induce cardiomyocytes to undergo auxotonic and isometric contractions. We also observed that the beating cycle became longer at lower temperatures, and the beating stopped at around 20 degrees C. This is a property similar to that of many mammalian hearts.
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