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Developing cell-culture-independent evaluation method for elucidating virus removal during drinking water treatment and advanced treatments enabling efficient virus removal

Research Project

Project/Area Number 18H03790
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 22:Civil engineering and related fields
Research InstitutionHokkaido University

Principal Investigator

Matsushita Taku  北海道大学, 工学研究院, 准教授 (30283401)

Co-Investigator(Kenkyū-buntansha) 松井 佳彦  北海道大学, 工学研究院, 教授 (00173790)
白崎 伸隆  北海道大学, 工学研究院, 准教授 (60604692)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥43,940,000 (Direct Cost: ¥33,800,000、Indirect Cost: ¥10,140,000)
Fiscal Year 2021: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2020: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2019: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
Fiscal Year 2018: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Keywords土木環境システム / ウイルス / 浄水処理
Outline of Final Research Achievements

DNA encapsulation in norovirus VLPs was successfully achieved by modifying foreign DNA onto gold nanoparticles in advance. Laboratory-scale experiments with the VLPs revealed that the removal of norovirus was comparable to that of PMMoV. Surveys at full-scale drinking water treatment plants showed that the removal of PMMoV during coagulation, sedimentation, and sand filtration processes was 0.9-2.7 logs, suggesting that the removal of native norovirus at the full-scale plants is expected to be 0.9-2.7 logs.

Academic Significance and Societal Importance of the Research Achievements

本研究で構築した手法は、いかなる「培養困難な水系ヒト感染性ウイルス」に対しても適用可能であるため、これまで浄水処理性を調べることができなかった他の多くの培養困難な水系ヒト感染ウイルスの浄水処理性の評価が可能となり、大きな波及効果が期待できる。本研究の成果は、実処理場の技術者にも大きなインパクトを与えるとともに、ウイルスの水道水質基準への組み込みについての議論にも大きく貢献する。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (13 results)

All 2022 2021 2020 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (11 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Evaluation of reduction efficiencies of pepper mild mottle virus and human enteric viruses in full-scale drinking water treatment plants employing coagulation-sedimentation-rapid sand filtration or coagulation-microfiltration2022

    • Author(s)
      Shirakawa, D., Shirasaki, N., Matsushita, T., Matsui, Y., Yamashita, R., Matsumura, T. and Koriki, S.
    • Journal Title

      Water Research

      Volume: 213 Pages: 118160-118160

    • DOI

      10.1016/j.watres.2022.118160

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suitability of pepper mild mottle virus as a human enteric virus surrogate for assessing the efficacy of thermal or free-chlorine disinfection processes by using infectivity assays and enhanced viability PCR2020

    • Author(s)
      Shirasaki N.、Matsushita T.、Matsui Y.、Koriki S.
    • Journal Title

      Water Research

      Volume: 186 Pages: 116409-116409

    • DOI

      10.1016/j.watres.2020.116409

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] Evaluating the efficacy of drinking water treatment processes to remove and inactivate human sapovirus: Application of in vitro cell-culture method2022

    • Author(s)
      Qiuhan Hu, Daiki Shirakawa, Nobutaka Shirasaki, Hirotaka Takagi, Tomoichiro Oka, Taku Matsushita and Yoshihiko Matsui
    • Organizer
      第56回日本水環境学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 低圧膜ろ過処理におけるウイルスの除去性:実浄水処理場における調査および室内添加実験の実施による評価2021

    • Author(s)
      松村拓哉, 白川大樹, 高力聡史, 白崎伸隆, 松下拓, 松井佳彦
    • Organizer
      第55回日本水環境学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Evaluation of virus reduction efficiency in coagulation-microfiltration by a full-scale study and lab-scale experiments2020

    • Author(s)
      Shirakawa, D., Shirasaki, N., Matsumura, T., Koriki, S., Matsushita, T. and Matsui, Y.
    • Organizer
      The Water and Environment Technology Conference 2020-Online
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 凝集沈澱-砂ろ過処理におけるウイルスの除去性 -実浄水処理場における調査および室内添加実験の実施による評価-2020

    • Author(s)
      白川大樹, 山下玲菜, 髙力聡史, 松村拓哉, 白崎伸隆, 松下拓, 松井佳彦
    • Organizer
      日本水道協会令和2年度全国会議(水道研究発表会)
    • Related Report
      2020 Annual Research Report
  • [Presentation] 膜ろ過浄水施設におけるウイルスの処理性評価:陽電荷膜と限外ろ過膜を組み合わせたウイルス濃縮法の適用2020

    • Author(s)
      白崎伸隆, 松村拓哉, 白川大樹, 高力聡史, 松下拓, 松井佳彦
    • Organizer
      第23回日本水環境学会シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 外来遺伝子を封入した人工合成ウイルス様粒子の創製:培養困難なノロウイルスの浄水処理性評価への適用2020

    • Author(s)
      白川大樹, 白崎伸隆, 松下拓, 松井佳彦
    • Organizer
      第54回日本水環境学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Evaluation of virus removal efficiency in an actual drinking water treatment plant by using a novel virus concentration method and pepper mild mottle virus as a process indicator.2019

    • Author(s)
      Shirakawa, D., Shirasaki, N., Yamashita, R., Koriki, S., Matsumura, T., Matsushita, T. and Matsui, Y.
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 凝集-膜ろ過処理を導入した実浄水処理場におけるウイルスの処理性評価2019

    • Author(s)
      松村拓哉, 高力聡史, 白川大樹, 白崎伸隆, 松下拓, 松井佳彦
    • Organizer
      第27回衛生工学シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] PMAxx-Enhancer-PCR 法による水道原水中の感染性ウイルスの選択的定量2019

    • Author(s)
      高力聡史, 白崎伸隆, 松下拓, 松井佳彦
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] トウガラシ微斑ウイルスを挙動指標とした膜ろ過浄水施設におけるウイルスの処理性評価2019

    • Author(s)
      松村拓哉, 高力聡史, 白川大樹, 白崎伸隆, 松下拓, 松井佳彦
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 培養困難なウイルスの浄水処理性評価に向けた遺伝子封入ウイルス様粒子の創製2018

    • Author(s)
      白川大樹, 白崎伸隆, 松下拓, 松井佳彦
    • Organizer
      第26回衛生工学シンポジウム
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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