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Four-dimensional multimodal optical microscopy with nonlinear holographic parallel manipulation of cells

Research Project

Project/Area Number 18H03888
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 30:Applied physics and engineering and related fields
Research InstitutionKobe University

Principal Investigator

Matoba Osamu  神戸大学, 先端融合研究環, 教授 (20282593)

Co-Investigator(Kenkyū-buntansha) 全 香玉  神戸大学, システム情報学研究科, 助教 (40814778)
粟辻 安浩  京都工芸繊維大学, 電気電子工学系, 教授 (80293984)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥30,940,000 (Direct Cost: ¥23,800,000、Indirect Cost: ¥7,140,000)
Fiscal Year 2018: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Keywordsマルチモーダルイメージング / 4次元イメージング / ディジタルホログラフィー / 強度輸送方程式 / 計算機ホログラフィー / マルチモーダル顕微鏡 / 非線形細胞観察技術 / 非線形細胞操作技術 / 蛍光 / 位相
Outline of Final Research Achievements

In order to construct a four-dimensional multimodal microscope with nonlinear holographic parallel manipulation of cells, we constructed four-dimensional multimodal optical microscopes with simultaneous observation of phase and fluorescence in three-dimensional space and one-dimensional time using digital holography or transport of intensity equation, and applied them to animal and plant cells. In addition, we introduced a technique of creating multi-spots using computer-generated holography for inducing stem cells in plant by parallel cell processing such as laser ablation.

Academic Significance and Societal Importance of the Research Achievements

光技術によるイメージング情報は生命科学や物理学など多くの研究分野の進展に役立ってきた。本研究では,位相と蛍光の2つの物理量を同時に空間3次元と時間1次元の4次元観察することを可能にし,動植物において生きた細胞観察に適用できることを実証した。これらの研究成果は,生命科学研究に有用なツールを提供することに繋がる。また,観察だけでなく植物の幹細胞化誘導に向けた光操作機能を有するシステムが完成すれば基礎研究,応用研究に役立つものと期待できる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (32 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 3 results) Presentation (21 results) (of which Int'l Joint Research: 16 results,  Invited: 14 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Department of Physics/ChouaibDoukkali University(モロッコ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Centro de Investigaciones en Optica(メキシコ)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Multimodal microscopy: Fast acquisition of quantitative phase and fluorescence imaging in 3D space2021

    • Author(s)
      Xiangyu Quan, Manoj Kumar, Sudheesh K Rajput, Yosuke Tamada, Yasuhiro Awatsuji, Osamu Matoba
    • Journal Title

      IEEE Journal of Selected Topics in Quantum Electronics

      Volume: 27 Issue: 4 Pages: 6800911-6800911

    • DOI

      10.1109/jstqe.2020.3038403

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Digital holographic multimodal cross-sectional fluorescence and quantitative phase imaging system2020

    • Author(s)
      Kumar M, Quan X, Awatsuji Y, Tamada Y, Matoba O
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 7580-7580

    • DOI

      10.1038/s41598-020-64028-x

    • NAID

      120006882492

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single-shot common-path off-axis dual-wavelength digital holographic microscopy2020

    • Author(s)
      Manoj Kumar, Xiangyu Quan, Yasuhiro Awatsuji, Yosuke Tamada, Osamu Matoba
    • Journal Title

      Applied Optics

      Volume: 59 Issue: 24 Pages: 7144-7152

    • DOI

      10.1364/ao.395001

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Common-path multimodal three-dimensional fluorescence and phase imaging system2020

    • Author(s)
      Kumar M, Quan X, Awatsuji Y, Cheng C, Hasebe M, Tamada Y, Matoba O
    • Journal Title

      Journal of Biomedical Optics

      Volume: 25 Issue: 03 Pages: 032010-032010

    • DOI

      10.1117/1.jbo.25.3.032010

    • NAID

      120007017120

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Digital four-step phase-shifting technique from a single fringe pattern using Riesz transform2019

    • Author(s)
      Tounsi Yassine、Kumar Manoj、Siari Ahmed、Mendoza-Santoyo Fernando、Nassim Abdelkrim、Matoba Osamu
    • Journal Title

      Optics Letters

      Volume: 44 Issue: 14 Pages: 3434-3434

    • DOI

      10.1364/ol.44.003434

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Speckle denoising by variant nonlocal means methods2019

    • Author(s)
      Tounsi Yassine、Kumar Manoj、Nassim Abdelkrim、Mendoza-Santoyo Fernando、Matoba Osamu
    • Journal Title

      Applied Optics

      Volume: 58 Issue: 26 Pages: 7110-7110

    • DOI

      10.1364/ao.58.007110

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 3D stimulation and imaging-based functional optical microscopy (SIFOM) of biological cells2018

    • Author(s)
      Xiangyu Quan, Manoj Kumar, Osamu Matoba, Yasuhiro Awatsuji, Yoshio Hayasaki, Satoshi Hasegawa, and Hiroki Wake
    • Journal Title

      Optics Letters

      Volume: 43 Issue: 21 Pages: 5447-5450

    • DOI

      10.1364/ol.43.005447

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Multi-wavelength digital holographic microscopy for bio-imaging and applications2020

    • Author(s)
      Manoj Kumar, Xiangyu Quan, Yasuhiro Awatsuji, and Osamu Matoba
    • Organizer
      6th Biomedical and Sensing Conference (BISC)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Stable multimodal three-dimensional imaging2020

    • Author(s)
      Manoj Kumar, Osamu Matoba, Xiangyu Quan, Yosuke Tamada, Yasuhiro Awatsuji
    • Organizer
      OSA Imaging and Applied Optics Congress
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 超深度ライトフィールド顕微鏡を用いた蛍光ビーズ記録2020

    • Author(s)
      新家 涼,Sudheesh K. Rajput,全 香玉,的場 修
    • Organizer
      日本光学会年次学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 空間光変調技術を用いた蛍光顕微鏡2020

    • Author(s)
      的場 修,全 香玉,Manoj Kumar,粟辻 安浩
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Active 3D fluorescence imaging based on holography2019

    • Author(s)
      O. Matoba, X. Quan, M. Kumar, Y. Awatsuji
    • Organizer
      SPIE Three-Dimensional Imaging, Visualization, and Display 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Observation of Plant Cell by Holographic 3D Illumination and Imaging Functional Optical Microscopy2019

    • Author(s)
      M. Kumar, X. Quan, Y. Awatsuji, Y. Tamada, O. Matoba
    • Organizer
      OSA Digital Holography and 3-D Imaging Topical Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Holographic 3D Stimulation and Observation for Neuroscience2019

    • Author(s)
      O. Matoba, X. Quan, Manoj Kumar, Y. Awatsuji
    • Organizer
      Workshop on Information Optics 2019 (WIO2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multimodal digital holography for live plant cell imaging2019

    • Author(s)
      O. Matoba, X. Quan, M. Kumar, Y. Awatsuji
    • Organizer
      SPIE Photonics Asia
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 3D Fluorescence Imaging Techniques for Biomedical Applications2019

    • Author(s)
      O. Matoba, M. Kumar, S. K. Rajput, X. Quan, and Y. Awatsuji
    • Organizer
      International Workshop on Holography and Related Technologies 2019 (IWH2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multimodal imaging based digital holography2019

    • Author(s)
      M. Kumar, O. Matoba, X. Quan, S. K. Rajput, and Y. Awatsuji
    • Organizer
      SPIE Future Sensing Technologies
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single-shot high-stable common-path off-axis digital holographic microscope2019

    • Author(s)
      M. Kumar, X. Quan, Y. Awatsuji, Y. Tamada, O. Matoba
    • Organizer
      日本光学会年次学術講演会2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] 蛍光オフアクシスデジタルホログラフィーと空間コヒーレンスの考察2019

    • Author(s)
      全 香玉,マノジ クマー, 柿田 康孝, 的場 修, 粟辻 安浩
    • Organizer
      日本光学会年次学術講演会2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] 生体培養細胞の三次元観察に向けたホログラフィック刺激システム2019

    • Author(s)
      小管 啓仁,全 香玉,森田 光洋,的場 修
    • Organizer
      日本光学会年次学術講演会2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] Three-dimensional imaging based on common-path off-axis incoherent digital holography2018

    • Author(s)
      Osamu Matoba
    • Organizer
      SPIE, Three-Dimensional Imaging, Visualization, and Display 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multimodal digital holographic microscopy for simultaneous phase and fluorescence imaging2018

    • Author(s)
      Xiangyu Quan
    • Organizer
      SPIE, The 4th Biomedical Imaging and Sensing Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multi-parameter imaging based on digital holography2018

    • Author(s)
      Osamu Matoba
    • Organizer
      The 10th International Conference on Digital Image Processing (ICDIP2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multimodal digital holographic microscopy and applications on live cell imaging2018

    • Author(s)
      Xiangyu Quan
    • Organizer
      The International Conference on 3D Systems & Applications 2018 (3DSA2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multi-dimensional digital holographic microscopy2018

    • Author(s)
      Osamu Matoba
    • Organizer
      Optical Design and Testing VIII, Photonics Asia 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A New Type of Microscopy for Light Stimulation and 3D Imaging2018

    • Author(s)
      Xiangyu Quan
    • Organizer
      International Workshop on Holography and Related Technologies 2018 (IWH2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Applications of Digital Holography to Biology and Sound Field Imaging2018

    • Author(s)
      Osamu Matoba
    • Organizer
      PHOTONICS-2018: International Conference on Fiber Optics and Photonics
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Multi-physical parameter imaging based on digital holography2018

    • Author(s)
      Osamu Matoba
    • Organizer
      17th Workshop on Information Optics (WIO2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] 神戸大学システム計測研究室ホームページ

    • URL

      http://www.lab.kobe-u.ac.jp/csi-applied-optics/index.html

    • Related Report
      2020 Annual Research Report
  • [Patent(Industrial Property Rights)] ホログラフィック3次元マルチスポット光刺激装置及び方法2018

    • Inventor(s)
      的場 修,全 香玉,和氣 弘明
    • Industrial Property Rights Holder
      神戸大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-130309
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2022-01-27  

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