Single cell mapping of epigenetic modifications through super-nucleosome construction
| Project/Area Number |
18H03931
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Medium-sized Section 37:Biomolecular chemistry and related fields
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Okamoto Akimitsu 東京大学, 先端科学技術研究センター, 教授 (60314233)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥44,200,000 (Direct Cost: ¥34,000,000、Indirect Cost: ¥10,200,000)
Fiscal Year 2020: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2019: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2018: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
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| Keywords | ヌクレオソーム / DNA / クロマチン / ヒストン / パラジウム / ルテニウム / ペプチド / タンパク質化学合成 / エピジェネティクス / タンパク質合成 / 遺伝子制御 / 化学合成 / 合成化学 / 1細胞イメージング |
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| Outline of Final Research Achievements |
(1) Establishment of a chemical synthesis method for artificial nucleosomes: We established a new peptide ligation method using organometallic complexes, and synthesized four types of core histones (H2A, H2B, H3, and H4) and linker histone H1.
(2) Creation of supernucleosomes: In order to discover the essence of the regulation of cell functions by atoms, we investigated protein modification methods and established a method for the chemical synthesis of "supernucleosomes" with functions not found in nature.
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| Academic Significance and Societal Importance of the Research Achievements |
独自の化学合成の確立を通して、天然にはない標識や反応性官能基、複数の複雑な機能性ユニットをヒストン構造に入れることが可能になるので、機能解明を支援する創造的スーパーヌクレオソームを作成して未知のヌクレオソーム機能を明らかにすることができる。これによって、ヌクレオソーム内DNAへ働きかけることができる新たな抗がん剤設計などに利用することができる。
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Report
(4 results)
Research Products
(51 results)
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[Journal Article] Single cell array enclosed with a photodegradable hydrogel in microwells for image-based cell classification and selective photorelease of cells2020
Author(s)
Yamaguchi, S.; Takagi, R.; Hosogane, T.; Ohashi, Y.; Sakai, Y.; Sakakihara, S.; Iino, R.; Tabata, K.; Noji, H.; Okamoto, A.
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Journal Title
ACS Appl. Bio Mater.
Volume: 3
Issue: 9
Pages: 5887-5895
DOI
Related Report
Peer Reviewed
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[Journal Article] Acetylation-modulated communication between the H3 N-terminal tail domain and the intrinsically disordered H1 C-terminal domain2020
Author(s)
Hao, F; Murphy, K. J.; Kujirai, T.; Kamo, N.; Kato, J.; Koyama, M.; Okamato, A.; Hayashi, G.; Kurumizaka, H.; Hayes, J. J.
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Journal Title
Nucleic Acids Res.
Volume: 48
Issue: 20
Pages: 11510-11520
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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