| Budget Amount *help |
¥44,200,000 (Direct Cost: ¥34,000,000、Indirect Cost: ¥10,200,000)
Fiscal Year 2020: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2019: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2018: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
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| Outline of Final Research Achievements |
In this study, we developed knock-in mice that incorporate photoactivatable DNA recombination system, named PA-Cre, into their chromosomes and adeno-associated viral vectors (AAVs) encoding PA-Cre, thereby developing a new cell analysis technology through in vivo verification of PA-Cre in living mice. In addition, we also developed a photoactivatable gene activation system that exhibits a genomic reaction upon light illumination, and validated it at the cellular level. These technologies are expected to contribute to the elucidation of various cell lineages and gene functions in vivo, which have been difficult to achieve with conventional technologies.
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