| Budget Amount *help |
¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000)
Fiscal Year 2020: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2019: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2018: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
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| Outline of Final Research Achievements |
We have previously developed our original artificial nucleic acid, D-aTNA, which cross-pairs with neither natural D-DNA nor D-RNA. In contrast to D-aTNA, our SNA can cross-pair with D-aTNA, D-DNA, and D-RNA. With these artificial nucleic acids, a new signal amplification system was designed by combining D-aTNA amplification circuit with SNA interface. The D-aTNA amplification circuit can be triggered by natural D-RNA that is orthogonal to D-aTNA because interface SNA can convert input D-RNA into D-aTNA output. By using this system, we have successfully developed robust signal amplification system that can detect small amount of microRNA (miR21) in the presence of contaminating DNA and RNA.
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