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Comprehensive understanding of nitrate signaling in plants

Research Project

Project/Area Number 18H03940
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 38:Agricultural chemistry and related fields
Research InstitutionThe University of Tokyo

Principal Investigator

Yanagisawa Shuichi  東京大学, 大学院農学生命科学研究科(農学部), 教授 (20222359)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2021: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2020: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2019: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2018: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywords窒素栄養 / 硝酸シグナル / 窒素応答 / 遺伝子発現制御 / 転写因子 / 植物 / 硝酸応答 / 遺伝子発現 / シグナル伝達 / 窒素 / 栄養応答 / 栄養シグナル / 転写カスケード / 窒素代謝 / NLP転写因子
Outline of Final Research Achievements

In plants, nitrate acts as a signal and induces changes in gene expression profiles, metabolic balances, etc., referred to as nitrate responses. In this study, the physiological roles of nitrate as a signal were investigated by identifying new target genes of NLP transcription factors that govern nitrate responses and clarifying the functions of the new target genes. Identification of the aspartate oxidase gene required for de novo NAD+ biosynthesis and two homeodomain-leucine zipper class I transcription factors as direct target genes of the NLP transcription factors revealed that nitrate-dependent coordinated regulation of N assimilation and other metabolic pathways is essential to optimizing growth in response to N nutrient conditions.

Academic Significance and Societal Importance of the Research Achievements

硝酸シグナルが窒素同化と他の代謝経路を同調的に制御していることが実証され、また、このような同調的制御が植物成長の最適化に重要であることも示されたことから、植物における硝酸シグナルの役割の理解が大きく進んだ。硝酸シグナルの新しい役割として葉緑体と光化学系の維持があることが明らかとなったこと、また、硝酸シグナルの伝達を強化すると光エネルギーの利用向上が起こる可能性があることを示したことは社会的意義も大きい。

Report

(5 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (50 results)

All 2022 2021 2020 2019 2018

All Journal Article (16 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 16 results,  Open Access: 7 results) Presentation (34 results) (of which Int'l Joint Research: 3 results,  Invited: 7 results)

  • [Journal Article] Arabidopsis nitrate-induced aspartate oxidase gene expression is necessary to maintain metabolic balance under nitrogen nutrient fluctuation2022

    • Author(s)
      Moriaki Saito, Mineko Konishi, Atsuko Miyagi, Yasuhito Sakuraba, Maki Kawai-Yamada, Shuichi Yanagisawa
    • Journal Title

      Communications Biology

      Volume: 5 Issue: 1 Pages: 432-432

    • DOI

      10.1038/s42003-022-03399-5

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Arabidopsis NLP7-HB52/54-VAR2 pathway modulates energy utilization in diverse light and nitrogen conditions2022

    • Author(s)
      Ariga Takuto、Sakuraba Yasuhito、Zhuo Mengna、Yang Mailun、Yanagisawa Shuichi
    • Journal Title

      Current Biology

      Volume: 32 Issue: 24 Pages: 5344-5353

    • DOI

      10.1016/j.cub.2022.10.024

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] RWP-RK domain-containing transcription factors in the Viridiplantae: biology and phylogenetic relationships2022

    • Author(s)
      Sakuraba Yasuhito、Zhuo Mengna、Yanagisawa Shuichi
    • Journal Title

      Journal of Experimental Botany

      Volume: 73 Issue: 13 Pages: 4323-4337

    • DOI

      10.1093/jxb/erac229

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 2. Mechanism of nitrogen transport in plants2021

    • Author(s)
      木羽 隆敏, 小西 美稲子, 柳澤 修一
    • Journal Title

      Japanese Journal of Soil Science and Plant Nutrition

      Volume: 92 Issue: 2 Pages: 76-91

    • DOI

      10.20710/dojo.92.2_76

    • NAID

      130008024162

    • ISSN
      0029-0610, 2424-0583
    • Year and Date
      2021-04-05
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ribosome biogenesis factor OLI2 and its interactor BRX1-2 are associated with morphogenesis and lifespan extension in <i>Arabidopsis thaliana</i>2021

    • Author(s)
      Shugo Maekawa, Shuichi Yanagisawa
    • Journal Title

      Plant Biotechnology

      Volume: 38 Issue: 1 Pages: 117-125

    • DOI

      10.5511/plantbiotechnology.20.1224a

    • NAID

      130008002860

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2021-03-25
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Enhanced NRT1.1/NPF6.3 expression in shoots improves growth under nitrogen deficiency stress in Arabidopsis2021

    • Author(s)
      Sakuraba Yasuhito、Chaganzhana、Mabuchi Atsushi、Iba Koh、Yanagisawa Shuichi
    • Journal Title

      Communications Biology

      Volume: 4 Issue: 1 Pages: 256-256

    • DOI

      10.1038/s42003-021-01775-1

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Nitrate-responsive NIN-like protein (NLP) transcription factors perform unique and redundant roles in Arabidopsis2021

    • Author(s)
      Konishi, M., Okitsu, T., and Yanagisawa, S.
    • Journal Title

      J. Exp. Bot.

      Volume: 72 Issue: 15 Pages: 5735-5750

    • DOI

      10.1093/jxb/erab246

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nitrate‐inducible NIGT1 proteins modulate phosphate uptake and starvation signalling via transcriptional regulation of SPX genes2020

    • Author(s)
      Ueda Yoshiaki、Kiba Takatoshi、Yanagisawa Shuichi
    • Journal Title

      The Plant Journal

      Volume: 102 Issue: 3 Pages: 448-466

    • DOI

      10.1111/tpj.14637

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Gene regulatory network and its constituent transcription factors that control nitrogen deficiency responses in rice2020

    • Author(s)
      Ueda Yoshiaki、Ohtsuki Namie、Kadota Koji、Tezuka Ayumi、Nagano Atsushi J.、Kadowaki Taro、Kim Yonghyun、Miyao Mitsue、Yanagisawa Shuichi
    • Journal Title

      New Phytologist

      Volume: in press Issue: 5 Pages: 1434-1452

    • DOI

      10.1111/nph.16627

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intercellular and intracellular colonization of Azorhizobium caulinodans in Arabidopsis thaliana under nitrogen deficient conditions2020

    • Author(s)
      Kurumisawa Keigo、Ishizuna Fumiko、Matsuoka Jun-Ichi、Ogawa Tetsuhiro、Morohashi Kengo、Aono Toshihiro
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 67 Issue: 1 Pages: 71-79

    • DOI

      10.1080/00380768.2020.1842139

    • NAID

      210000158619

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Jasmonate-Activated MYC2-Dof2.1-MYC2 Transcriptional Loop Promotes Leaf Senescence in Arabidopsis2019

    • Author(s)
      Zhuo Mengna、Sakuraba Yasuhito、Yanagisawa Shuichi
    • Journal Title

      The Plant Cell

      Volume: 32 Issue: 1 Pages: 242-262

    • DOI

      10.1105/tpc.19.00297

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The role of protein-protein interactions mediated by the PB1 domain of NLP transcription factors in nitrate-inducible gene expression2019

    • Author(s)
      Konishi, M. and Yanagisawa, S.
    • Journal Title

      BMC Plant Biology

      Volume: 19 Issue: 1 Pages: 90-90

    • DOI

      10.1186/s12870-019-1692-3

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Delineation of Nitrogen Signaling Networks: Computational Approaches in the Big Data Era2019

    • Author(s)
      Ueda Yoshiaki、Yanagisawa Shuichi
    • Journal Title

      Molecular Plant

      Volume: 12 Issue: 2 Pages: 150-152

    • DOI

      10.1016/j.molp.2019.01.008

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Perception, transduction, and integration of nitrogen and phosphorus nutritional signals in the transcriptional regulatory network in plants2019

    • Author(s)
      Ueda Yoshiaki、Yanagisawa Shuichi
    • Journal Title

      Journal of Experimental Botany

      Volume: 70 Issue: 15 Pages: 3709-3717

    • DOI

      10.1093/jxb/erz148

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A phytochrome B-mediated regulatory mechanism of phosphorus acquisition2018

    • Author(s)
      Sakuraba, Y., Kanno, S., Mabuchi, A., Monda, K., Iba, K., and Yanagisawa, S.
    • Journal Title

      Nature Plants

      Volume: 4 Issue: 12 Pages: 1089-1101

    • DOI

      10.1038/s41477-018-0294-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of a Brix Domain-Containing Ribosome Biogenesis Factor ARPF2 and its Interactor ARRS1 Causes Morphological Changes and Lifespan Extension in Arabidopsis thaliana2018

    • Author(s)
      Shugo Maekawa, Yoshiaki Ueda, Shuichi Yanagisawa.
    • Journal Title

      Front. Plant Sci.

      Volume: 9 Pages: 1177-1177

    • DOI

      10.3389/fpls.2018.01177

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] NIGT1-centered Transcriptional Network for the Balanced Acquisition of Nitrogen and Phosphorus2022

    • Author(s)
      Shuichi YANAGISAWA
    • Organizer
      The 86th Annual Meeting of the Botanical Society of Japan
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] The role of Dof1.7 transcription factor in NIGT1-regulated nitrogen deficiency responses in Arabidopsis2022

    • Author(s)
      Mengna Zhuo, Yasuhito Sakuraba, Shuichi Yanagisawa
    • Organizer
      第63回日本植物生理学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] グルタミンによる窒素応答関連遺伝子の発現抑制におけるLBDタンパク質の役割2022

    • Author(s)
      鳥井 要佑、小西 美稲子、櫻庭 康仁、柳澤 修一
    • Organizer
      第63回日本植物生理学会つくば年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] The Role of Dof1.7 Transcription Factor in NIGT1-Mediated Nitrogen Starvation Responses in Arabidopsis2021

    • Author(s)
      Mengna Zhuo, Yasuhito Sakuraba, Shuichi Yanagisawa
    • Organizer
      日本土壌肥料学会 2021年度北海道大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] イネの窒素欠乏応答ネットワークにおいて重要な役割を果たす転写因子の機能解析2021

    • Author(s)
      大槻 並枝、植田 佳明、櫻庭 康仁、柳澤 修一
    • Organizer
      日本土壌肥料学会 2021年度北海道大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] An Arabidopsis NRT1.1 allele is a superior allele conferring better nitrogen use under nitrogen-deficient conditions2020

    • Author(s)
      Chaganzhana, Yasuhito Sakuraba, Shuichi Yanagisawa
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナにおける赤色光シグナル伝達による硝酸獲得の調節機構2020

    • Author(s)
      尾上佑真、櫻庭康仁、柳澤修一
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナの硝酸応答時におけるNRT2.1の発現パターンの数理モデル2020

    • Author(s)
      植田佳明、柳澤修一
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Involvement of Dof1.7 transcriptional repressor in the NIGT1-regulated nitrogen starvation responses in Arabidopsis2020

    • Author(s)
      Mengna Zhuo, Yasuhito Sakuraba, Shuichi Yanagisawa
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Analysis of the degradation mechanism of NIN-LIKE PROTEIN (NLP) transcription factors responsible for nitrate responses in plants2020

    • Author(s)
      Takuto Ariga, Yasuhito Sakuraba, Mineko Konishi, and Shuichi Yanagisawa
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] グルタミンによるNRT2.1の発現抑制の分子機構2020

    • Author(s)
      小西美稲子、郭鵬程、柳澤修一
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] The role of Dof1.7 transcription factor in NIGT1-regulated nitrogen starvation responses in Arabidopsis2020

    • Author(s)
      Mengna Zhuo, Yasuhito Sakuraba, Shuichi Yanagisawa
    • Organizer
      日本土壌肥料学会 2020年度 岡山大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナにおける硝酸シグナルによる葉緑体発達制御の可能性の検討2020

    • Author(s)
      有賀琢人、櫻庭康仁 、柳澤修一
    • Organizer
      日本土壌肥料学会 2020年度 岡山大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナのNIGT1転写因子間の相互作用が標的配列の認識と栄養応答に及ぼす影響2020

    • Author(s)
      植田佳明、野崎翔平、宮川拓也、木羽隆敏、田之倉優、柳澤修一
    • Organizer
      日本土壌肥料学会 2020年度 岡山大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 低窒素環境において生育向上をもたらすNRT1.1硝酸輸送体遺伝子プロモーター中の自然突然変異2020

    • Author(s)
      櫻庭康仁、Chaganzahana、馬渕敦士、射場厚、柳澤修一
    • Organizer
      日本土壌肥料学会 2020年度 岡山大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナにおける赤色光シグナルによる硝酸態窒素獲得の調節機構2020

    • Author(s)
      尾上佑真、櫻庭康仁、柳澤修一
    • Organizer
      日本土壌肥料学会 2020年度 岡山大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 硝酸応答とニコチンアミドアデニンジヌクレオチド合成2019

    • Author(s)
      柳澤修一
    • Organizer
      第4回植物栄養研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Transcriptional cascades maintaining the balance of N and P acquisition2019

    • Author(s)
      柳澤修一
    • Organizer
      Nitrogen 2019 The 4th International Symposium on the Nitrogen Nutrition of Plants
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 多様な環境での栄養獲得・利用の最適化のための植物メカニズム2019

    • Author(s)
      柳澤修一
    • Organizer
      岡大植物研共同研究拠点ワークショップ
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナの窒素栄養飢餓応答に 関わるABCタンパク質の機能解析2019

    • Author(s)
      櫻庭康仁、 馬渕敦士、射場厚、 柳澤修一
    • Organizer
      日本土壌肥料学会 2019年度 静岡大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物の硝酸応答を担うNLP転写因子の分解機構の解析2019

    • Author(s)
      有賀琢人、小西美稲子、柳澤修一
    • Organizer
      日本土壌肥料学会 2019年度静岡大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シロイヌナズナ硝酸応答性転写因子NLP2のNLP7とは異なる役割2019

    • Author(s)
      小西美稲子、沖津孝幸、柳澤修一
    • Organizer
      日本土壌肥料学会 2019年度静岡大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Possible involvement of Dof1.7 transcription factor in nitrogen-starvation responses in Arabidopsis2019

    • Author(s)
      Mengna Zhuo・櫻庭康仁・柳澤修一
    • Organizer
      日本土壌肥料学会 2019年度静岡大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 硝酸シグナルに応答した遺伝子発現誘導におけるNLP転写因子のPB1ドメインの役割2019

    • Author(s)
      小西美稲子,柳澤修一
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物におけるグルタミンを介した窒素応答のフィードバック制御の分子メカニズム2019

    • Author(s)
      Pengcheng Guo, Mineko Konishi, Shuichi Yanagisawa
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナにおける,硝酸シグナルによる NAD 生合成制御がもたらす広範な代謝動態2019

    • Author(s)
      斉藤守秋,宮城敦子,小西美稲子,川合真紀,柳澤修一
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Roles of the NIGT1-centered transcriptional cascade in the regulation of nitrate uptake and responses2019

    • Author(s)
      Mineko Konishi, Yoshiaki Ueda and Shuichi Yanagisawa
    • Organizer
      Plant and Animal Genome XXVII
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高等植物におけるグルタミンを介した窒素利用関連遺伝子の発現抑制の分子機構2018

    • Author(s)
      Guo Pengcheng, Mineko Konishi, Shuichi Yanagisawa
    • Organizer
      土壌肥料学会2018年度神奈川大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] NLP転写因子を介した硝酸シグナルによるNAD生合成制御メカニズム2018

    • Author(s)
      斉藤守秋、小西美稲子、柳澤修一
    • Organizer
      土壌肥料学会2018年度神奈川大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 硝酸応答性転写因子NLP7におけるPB1ドメインの役割の検討2018

    • Author(s)
      小西美稲子、柳澤修一
    • Organizer
      日本土壌肥料学会2018年度神奈川大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Modulation of methyl jasmonate signaling by nitrate-inducible Dof2.1 transcriptional activator in Arabidopsis2018

    • Author(s)
      Mengna Zhuo, Yasuhito Sakuraba, Shuichi Yanagisawa
    • Organizer
      日本土壌肥料学会2018年度神奈川大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 硝酸シグナル伝達とリン飢餓シグナル伝達の転写カスケードを介したクロストーク2018

    • Author(s)
      柳澤修一
    • Organizer
      日本植物学会第82回大会シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 赤色光シグナルによる植物栄養獲得の調節2018

    • Author(s)
      櫻庭康仁、柳澤修一
    • Organizer
      植物の栄養研究会 第4回研究交流会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Nutrient uptake-based phenomics and transcriptomic analyses using diverse rice accessions2018

    • Author(s)
      Yoshiaki Ueda, Koji Kadota, Ayumi Tezuka, Atsushi J. Nagano, Taro Kadowaki, Yonghyun Kim, Mitsue Miyao, Shuichi Yanagisawa
    • Organizer
      16th International Symposium on Rice Functional Genomics
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2024-01-30  

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