| Budget Amount *help |
¥43,940,000 (Direct Cost: ¥33,800,000、Indirect Cost: ¥10,140,000)
Fiscal Year 2022: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2021: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2020: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2019: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2018: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Outline of Final Research Achievements |
The project aimed to elucidate the biosynthetic enzymes and receptors of the mating hormones (α1 and α2) that control the sexual reproduction of Phytophthora, a crop disease fungus. Regarding biosynthetic enzymes, RNA-Seq was used to narrow down the cytochrome P450 (CYP) genes, which show differences in gene expression level between the A1 and A2 mating types. We succeeded in identifying 11-hydroxylase as one of the biosynthetic genes for α2 (=11,16-dihydroxyphytol). On the other hand, the receptors were narrowed down by structural similarity and nuclear receptors, etc., and one receptor candidate pair (94% homology) was found. The regulators of asexual reproduction that are responsible for the rapid epidemic was also investigated.
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