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Innovating seedling cultivation of endangered tree species by symbiotic microbes

Research Project

Project/Area Number 18H03955
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
Research InstitutionThe University of Tokyo

Principal Investigator

Nara Kazuhide  東京大学, 大学院新領域創成科学研究科, 教授 (60270899)

Co-Investigator(Kenkyū-buntansha) 松田 陽介  三重大学, 生物資源学研究科, 教授 (30324552)
酒井 敦  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (70353696)
金谷 整一  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (90353648)
岩泉 正和  国立研究開発法人森林研究・整備機構, 森林総合研究所 林木育種センター, 主任研究員 等 (50391701)
Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2022: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2021: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2020: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2019: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2018: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Keywords菌根菌 / 絶滅危惧樹木 / 外生菌根菌 / 生物多様性保全 / 外交配
Outline of Final Research Achievements

Ectomycorrhizal (ECM) fungi are symbiotic microbes colonizing tree roots and helping the growth of the host trees. In previous studies, we discovered new ECM fungi specific to endangered host trees and the application of these fungi to host conservation is expected. However, fruitbodies of these specific fungi are rarely attainable and difficult to use in the inoculation to hosts. In this study, we investigated the possibility of using soil spore banks, dormant spores in forest soil, for the inoculation. Our results showed that host seedlings were effectively colonized by these specific fungi by filtering out larger sclerotia of other competing fungi before the application of soil water solution to host seedlings. We also found that outcrossing induced by mixing spore banks from different sites at the inoculation can improve the function of these specific fungi, which suffer inbreeding depression in the small isolated populations.

Academic Significance and Societal Importance of the Research Achievements

樹木の成長と生存には菌根菌との共生が不可欠であるものの、絶滅危惧樹木の保全において菌根菌はこれまで全く考慮されていない。本研究では、対象とする絶滅危惧樹木にのみ共生し、その樹木の成長のみを助ける菌根菌の効果的な接種方法等を明らかにした。これによって、これまで困難であった絶滅危惧樹木の保全を大きく改善できる可能性がある。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (27 results)

All 2023 2022 2021 2020 2019

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results) Presentation (16 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Book (2 results)

  • [Journal Article] Spatial distribution of spore banks of ectomycorrhizal fungus, <i>Rhizopogon togasawarius</i>, at <i>Pseudotsuga japonica</i> forest boundaries2022

    • Author(s)
      Okada Keita Henry、Abe Hiroshi、Matsuda Yosuke、Nara Kazuhide
    • Journal Title

      Journal of Forest Research

      Volume: 印刷中 Issue: 4 Pages: 1-7

    • DOI

      10.1080/13416979.2021.2023386

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ヤクタネゴヨウのマツ材線虫病対策2022

    • Author(s)
      金谷整一
    • Journal Title

      樹木医学研究

      Volume: 印刷中

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Soil spore bank communities of ectomycorrhizal fungi in Pseudotsuga japonica forests and neighboring plantations2022

    • Author(s)
      Okada Keita Henry、Matsuda Yosuke
    • Journal Title

      Mycorrhiza

      Volume: 32 Issue: 1 Pages: 83-93

    • DOI

      10.1007/s00572-021-01065-y

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tuber iryudaense and T. tomentosum: Two new truffles encased in tomentose mycelium from Japan2021

    • Author(s)
      Kinoshita, A., Sasaki, H., Orihara, T., Nakajima, M., Nara, K.
    • Journal Title

      Mycologia

      Volume: 113 Issue: 3 Pages: 653-663

    • DOI

      10.1080/00275514.2021.1875709

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unique host effect of Tilia japonica on ectomycorrhizal fungal communities independent of the tree’s dominance: A rare example of a generalist host?2021

    • Author(s)
      Janowski D, Nara K
    • Journal Title

      Global Ecology and Conservation

      Volume: 31 Pages: e01863-e01863

    • DOI

      10.1016/j.gecco.2021.e01863

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ectomycorrhizal fungal communities in ice-age relict forests of Pinus pumila on nine mountains correspond to summer temperature2020

    • Author(s)
      Koizumi T, Nara K
    • Journal Title

      ISME Journal

      Volume: 14 Issue: 1 Pages: 189-201

    • DOI

      10.1038/s41396-019-0524-7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 屋久島の森林生態系と絶滅危惧種ヤクタネゴヨウの保全2020

    • Author(s)
      金谷整一・手塚賢至
    • Journal Title

      農業新時代

      Volume: 1 Pages: 30-37

    • NAID

      40022558008

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Towards the conservation of ectomycorrhizal fungi on endangered trees: native fungal species on Pinus amamiana are rarely conserved in trees planted ex situ2019

    • Author(s)
      Sugiyama Y, Murata M, Kanetani S, Nara K
    • Journal Title

      Mycorrhiza

      Volume: 29 Issue: 3 Pages: 195-205

    • DOI

      10.1007/s00572-019-00887-1

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of Rhizopogon assocated with Larix from northeastern Siberia: insights into host shift of ectomycorrhizal fungi2019

    • Author(s)
      Miyamoto Y, Maximov TC, Sugimoto A, Nara K
    • Journal Title

      Mycoscience

      Volume: 60 Issue: 5 Pages: 274-280

    • DOI

      10.1016/j.myc.2019.03.003

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 日本産ゴヨウマツの外生菌根菌群集構造2023

    • Author(s)
      阿部寛史・奈良一秀
    • Organizer
      第133回日本森林学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 日本のチョウセンゴヨウ林における外生菌根菌群集2023

    • Author(s)
      立田悠土・奈良一秀・阿部寛史・張 鵬翼
    • Organizer
      第133回日本森林学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 絶滅危惧樹木トガサワラが天然更新する環境条件2022

    • Author(s)
      酒井敦・安藤暁子・奈良一秀
    • Organizer
      第132回日本森林学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Do Tilia maximowicziana ectomycorrhizal communities in Hokkaido include a Tilia-specific ectomycorrhizal fungal species?2022

    • Author(s)
      Janowski D, Nara K
    • Organizer
      第133回日本森林学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 日本産ショウロ属の分子系統解析2022

    • Author(s)
      阿部寛史・花岡美保・奈良一秀
    • Organizer
      日本菌学会第66回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 絶滅危惧針葉樹ヤクタネゴヨウの遺伝的多様性と集団遺伝構造2022

    • Author(s)
      柴野達彦、金谷整一、手塚賢至、池亀寛治、大久保達彦、逢沢峰昭
    • Organizer
      日本森林学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 絶滅危惧樹木トガサワラが天然更新する環境条件2022

    • Author(s)
      酒井敦、安藤暁子、奈良一秀
    • Organizer
      日本森林学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 絶滅危惧樹木ヤクタネゴヨウ育苗のための外生菌根菌接種方法の検討2021

    • Author(s)
      大嶋健資・奈良一秀
    • Organizer
      土壌微生物学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 絶滅危惧種トガサワラの残存林周辺に潜在する外生菌根菌の空間分布2020

    • Author(s)
      岡田経太・松田陽介
    • Organizer
      第131回日本森林学会大会.名古屋大学(名古屋市)
    • Related Report
      2019 Annual Research Report
  • [Presentation] ヤクタネショウロの埋土胞子は何年生きるか:過去の松枯れ被害地から推定2020

    • Author(s)
      村田政穂・金谷整一・奈良一秀
    • Organizer
      第131回日本森林学会大会(名古屋大学, 3/27-3/30)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高知県安田川山希少個体群保護林におけるトガサワラの成長と更新2020

    • Author(s)
      酒井敦・安藤暁子・奈良一秀
    • Organizer
      第131回日本森林学会大会(名古屋大学, 3/27-3/30)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 絶滅危惧樹木ヤクタネゴヨウ保全のための外生菌根菌の遺伝的救助2020

    • Author(s)
      大嶋健資・杉山賢子・金谷整一・村田政穂・奈良一秀
    • Organizer
      第131回日本森林学会大会(名古屋大学, 3/27-3/30)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Molecular detections of soil inocula of an ectomycorrhizal fungus, Rhizopogon togasawariana, in Japanese Douglas-fir forests2019

    • Author(s)
      Okada K, Matsuda Y
    • Organizer
      Asian Mycological Congress 2019(AMC 2019), Mie Center for the Arts,Tsu, Mie, Oct 1-Oct4
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] What will happen to ectomycorrhizal fungi in fragmented forests? : population genetic implications from endangered or relict forests2019

    • Author(s)
      Nara K
    • Organizer
      The 10th International Workshop on Edible Mycorrhizal Mushroom (Oct. 20-25, Suwa, Nagano)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] What will happen to ectomycorrhizal fungi in fragmented forests? : population genetic implications from endangered or relict forests2019

    • Author(s)
      Nara K
    • Organizer
      The 10th International Workshop on Edible Mycorrhizal Mushroom (Oct. 20-25, Suwa, Nagano)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 埋土胞子の混合接種による遺伝的に分化した外 生菌根菌集団間の外交配の誘導2019

    • Author(s)
      大嶋健資・杉山賢子・金谷整一・村田政穂・奈良一秀
    • Organizer
      第130回日本森林学会大会(新潟コンベンションセンター, 3/20-23)
    • Related Report
      2018 Annual Research Report
  • [Book] 絶滅危惧種トガサワラの更新特性2023

    • Author(s)
      酒井敦
    • Total Pages
      20
    • Publisher
      国立研究開発法人 森林研究・整備機構
    • Related Report
      2022 Annual Research Report
  • [Book] トガサワラ.高知県レッドデータブック2022植物編2022

    • Author(s)
      酒井敦(分担執筆)
    • Total Pages
      288
    • Publisher
      高知県
    • Related Report
      2020 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2024-01-30  

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