| Budget Amount *help |
¥44,330,000 (Direct Cost: ¥34,100,000、Indirect Cost: ¥10,230,000)
Fiscal Year 2021: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2020: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2019: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2018: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
|
|---|
| Outline of Final Research Achievements |
The purpose of this project is to elucidate the mechanism regulating the reprogram of gene expression after fertilization by analyzing the dynamics and the function of histone variants. First, I found that H3.3 but not H3.1/H3.2 is abundant in the nuclei of 1-cell stage embryos in mice. Regarding H2A variants, only H2A.X and TH2A is abundant. Knock-down/out and overexpression of these proteins caused the developmental delay and/or arrest and the changes in the expression of some stage specific genes and chromatin structure, suggesting that these histone variants are involved in the regulation of reprogramming.
|
