Novel mechanisms of protein quality control mediated by the cooperation of ERp44 and zinc ions in the early secretory pathway

• Project/Area Number: 18H03978
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
• Research Institution: Tohoku University
• Principal Investigator: Inaba Kenji 東北大学, 多元物質科学研究所, 教授 (10423039)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000); Fiscal Year 2020: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000); Fiscal Year 2019: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000); Fiscal Year 2018: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
• Keywords: タンパク質品質管理 / 亜鉛イオン / 亜鉛輸送体 / ERp44 / 小胞体 / ゴルジ体 / Ｘ線結晶構造解析 / 細胞イメージング / 亜鉛トランスポーター / クライオ電子顕微鏡 / タンパク質品管理 / 蛋白質品質管理 / 亜鉛

Outline of Final Research Achievements

ERp44 is a PDI family member that plays an important role in protein quality control in the early secretory pathway. We recently discovered that zinc ions (Zn2+) regulate structure, affinity for client proteins and intracellular localization of ERp44, leading to its maximal client retention activity. We also developed an organelle-targettable Zn2+ fluorescent probe in collaboration with Prof. Shin Mizukami's group, and succeeded in quantification of labile Zn2+ concentration in the Golgi with the probe. By Zn2+ imaging under the conditions of systematic Zn2+ transporter (ZnT) knockdowns, we revealed that ZnT7 serves as a　ZnT that primarily regulates intracellular localization and function of ERp44. Thus, we established molecular and mechanistic basis of the ZnT-mediated Zn2+ homeostasis and ERp44-dependent protein quality control in the early secretory pathway.

Academic Significance and Societal Importance of the Research Achievements

細胞のタンパク質品質管理機構の異常は、アルツハイマー病などの神経変性疾患や糖尿病を引き起こすことが数多く報告されている。また亜鉛イオンの恒常性維持の破綻が、発育障害、免疫低下、皮膚病等のいわゆる亜鉛欠乏症を引き起こすことも広く知られる。本研究において我々は、小胞体とゴルジ体における、亜鉛イオンとpHによって制御される全く新しいタンパク質品質管理機構を解明した。さらに細胞内小器官の遊離亜鉛イオン濃度を定量することにも成功し、細胞の亜鉛恒常性維持機構に関する重要な知見を得た。本研究成果は上記の疾患の原因解明・創薬につながり、基礎細胞生物学のみならず、医学的にも非常に重要なものである。

Research Products

• [Int'l Joint Research] San Raffaele Scientific Institute(イタリア)
• [Int'l Joint Research] San Rafaelle Scientific Institute(イタリア)
• [Int'l Joint Research] Academia Sinica(台湾)
• [Int'l Joint Research] San Raffaele Scientific Institute(イタリア)
• [Journal Article] Distinct roles and actions of protein disulfide isomerase family enzymes in catalysis of nascent-chain disulfide bond formation (2021)
  • Author(s): Hirayama, C., Machida, K., Noi, K., Murakawa, T., Okumura, M., Ogura, T., Imataka, H., and Inaba, K.*
  • Journal Title: iScience Volume: 4 Issue: 4 Pages: 102296-102296
  • DOI: 10.1016/j.isci.2021.102296
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A unique leucine-valine adhesive motif supports structure and function of protein disulfide isomerase P5 via dimerization (2021)
  • Author(s): Okumura M, Kanemura S., Matsusaki M., Kinoshita M., Saio T., Ito D., Hirayama C., Kumeta H., Watabe M., Amagai Y., Lee Y. H., Akiyama S. and Inaba K.
  • Journal Title: Structure Volume: 29 Issue: 12 Pages: 1-14
  • DOI: 10.1016/j.str.2021.03.016
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cryo-EM structures of SERCA2b reveal the mechanism of regulation by the luminal extension tail (2020)
  • Author(s): Zhang. Y., Inoue, M., Tsutsumi, A., Watanabe, S., Nishizawa, T., Nagata, K., Kikkawa, M. and Inaba, K.*
  • Journal Title: Science Advances Volume: 6 Issue: 33
  • DOI: 10.1126/sciadv.abb0147
  • Peer Reviewed / Open Access
• [Journal Article] Quantitative Imaging of Labile Zn2+ in the Golgi Apparatus Using a Localizable Small-Molecule Fluorescent Probe (2020)
  • Author(s): Kowada Toshiyuki、Watanabe Tomomi、Amagai Yuta、Liu Rong、Yamada Momo、Takahashi Hiroto、Matsui Toshitaka、Inaba Kenji、Mizukami Shin
  • Journal Title: Cell Chemical Biology Volume: 27 Issue: 12 Pages: 1521-1531
  • DOI: 10.1016/j.chembiol.2020.09.003
  • Peer Reviewed
• [Journal Article] Characterization of the endoplasmic reticulum-resident peroxidases GPx7 and GPx8 shows the higher oxidative activity of GPx7 and its linkage to oxidative protein folding (2020)
  • Author(s): Kanemura S., Sofia E. F., Hirai N., Okumura M., Kadokura H. and Inaba K.
  • Journal Title: J. Biol. Chem. Volume: 295 Issue: 36 Pages: 12772-12785
  • DOI: 10.1074/jbc.ra120.013607
  • Peer Reviewed / Open Access
• [Journal Article] Observing the nonvectorial yet cotranslational folding of a multidomain protein, LDL receptor, in the ER of mammalian cells (2020)
  • Author(s): Kadokura Hiroshi、Dazai Yui、Fukuda Yo、Hirai Naoya、Nakamura Orie、Inaba Kenji
  • Journal Title: Proceedings of the National Academy of Sciences, USA Volume: 117 Issue: 28 Pages: 16401-16408
  • DOI: 10.1073/pnas.2004606117
  • Peer Reviewed / Open Access
• [Journal Article] Antipsychotic olanzapine-induced misfolding of proinsulin in the endoplasmic reticulum accounts for atypical development of diabetes (2020)
  • Author(s): Ninagawa Satoshi、Tada Seiichiro、Okumura Masaki、Inoguchi Kenta、Kinoshita Misaki、Kanemura Shingo、Imami Koshi、Umezawa Hajime、Ishikawa Tokiro、Mackin Robert B、Torii Seiji、Ishihama Yasushi、Inaba Kenji、Anazawa Takayuki、Nagamine Takahiko、Mori Kazutoshi
  • Journal Title: eLife Volume: 9 Pages: 23-31
  • DOI: 10.7554/elife.60970
  • NAID: 120006898101
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] PDI Family Members as Guides for Client Folding and Assembly (2020)
  • Author(s): Kanemura S., Matsusaki M., Inaba K. and Okumura M.
  • Journal Title: Int. J. Mol. Sci. Volume: 21 Issue: 24 Pages: 9351-9351
  • DOI: 10.3390/ijms21249351
  • Peer Reviewed / Open Access
• [Journal Article] Visualization of structural dynamics of protein disulfide isomerase enzymes in catalysis of oxidative folding and reductive unfolding (2020)
  • Author(s): Okumura, M., Noi, K. and Inaba, K.*
  • Journal Title: Curr. Opin. Struct. Biol. Volume: 66 Pages: 49-57
  • DOI: 10.1016/j.sbi.2020.10.004
  • Peer Reviewed / Open Access
• [Journal Article] Structural basis of sarco/endoplasmic reticulum Ca2+-ATPase 2b regulation via transmembrane helix interplay (2019)
  • Author(s): Michio Inoue, Nanami Sakuta, Satoshi Watanabe, Yuxia Zhang, Kunihito Yoshikaie, Yoshiki Tanaka, Ryo Ushioda, Yukinari Kato, Junichi Takagi, Tomoya Tsukazaki, Kazuhiro Nagata, Kenji Inaba
  • Journal Title: Cell Reports Volume: 27 Issue: 4 Pages: 1221-1230
  • DOI: 10.1016/j.celrep.2019.03.106
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] High-Resolution Crystal Structure of Arabidopsis FLOWERING LOCUS T Illuminates Its Phospholipid-Binding Site in Flowering (2019)
  • Author(s): Nakamura Yuki、Lin Ying-Chen、Watanabe Satoshi、Liu Yu-chi、Katsuyama Kentaro、Kanehara Kazue、Inaba Kenji
  • Journal Title: iScience Volume: 21 Pages: 577-586
  • DOI: 10.1016/j.isci.2019.10.045
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dynamic assembly of protein disulfide isomerase in catalysis of oxidative folding (2019)
  • Author(s): Okumura Masaki、Noi Kentaro、Kanemura Shingo、Kinoshita Misaki、Saio Tomohide、Inoue Yuichi、Hikima Takaaki、Akiyama Shuji、Ogura Teru、Inaba Kenji
  • Journal Title: Nature Chemical Biology Volume: 15 Issue: 5 Pages: 499-509
  • DOI: 10.1038/s41589-019-0268-8
  • Peer Reviewed
• [Journal Article] Protein Disulfide Isomerase family: from Proteostasis to Pathogenesis (2019)
  • Author(s): Matsusaki M.#, Kanemura S.#, Kinoshita M.#, Lee Y.H., Inaba K.*, and Okumura M.* (#equal contribution *corresponding authors)
  • Journal Title: Biochim Biophys Acta-general subjects Volume: in press Issue: 2 Pages: 30080-30087
  • DOI: 10.1016/j.bbagen.2019.04.003
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Coupling effects of thiol and urea-type groups for promotion of oxidative protein folding (2019)
  • Author(s): Shunsuke Okada, Motonori Matsusaki, Kenta AraiK , Yuji Hidaka, Kenji Inaba, Masaki Okumura, Takahiro Muraoka
  • Journal Title: Chemical Communications Volume: 55(6) Issue: 6 Pages: 759-762
  • DOI: 10.1039/c8cc08657e
  • Peer Reviewed
• [Journal Article] Zinc regulates ERp44-dependent protein quality control in the early secretory pathway (2019)
  • Author(s): Watanabe Satoshi、Amagai Yuta、Sannino Sara、Tempio Tiziana、Anelli Tiziana、Harayama Manami、Masui Shoji、Sorrentino Ilaria、Yamada Momo、Sitia Roberto、Inaba Kenji
  • Journal Title: Nature Communications Volume: 10 Issue: 1 Pages: 1-16
  • DOI: 10.1038/s41467-019-08429-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Methods to identify the substrates of thiol‐disulfide oxidoreductases (2019)
  • Author(s): Fujimoto Takushi、Inaba Kenji、Kadokura Hiroshi
  • Journal Title: Protein Science Volume: 28 Issue: 1 Pages: 30-40
  • DOI: 10.1002/pro.3530
  • Peer Reviewed / Open Access
• [Journal Article] Identification of the physiological substrates of PDIp, a pancreas-specific protein-disulfide isomerase family member (2018)
  • Author(s): Fujimoto Takushi、Nakamura Orie、Saito Michiko、Tsuru Akio、Matsumoto Masaki、Kohno Kenji、Inaba Kenji、Kadokura Hiroshi
  • Journal Title: Journal of Biological Chemistry Volume: 293 Issue: 48 Pages: 18421-18433
  • DOI: 10.1074/jbc.ra118.003694
  • Peer Reviewed / Open Access
• [Journal Article] Ero1-mediated reoxidation of PDI accelerates the folding of cone-snail toxins (2018)
  • Author(s): O'Brien H., Kanemura S., Okumura M., Baskin P., Bandyopadhyay P., Ellgaard L., Inaba K., and Safavi-Hemami H.*
  • Journal Title: Int. J. Mol. Sci. Volume: 11 Issue: 11 Pages: 3418-3418
  • DOI: 10.3390/ijms19113418
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization and optimization of two-chain folding pathways of insulin via native chain assembly (2018)
  • Author(s): Arai Kenta、Takei Toshiki、Shinozaki Reina、Noguchi Masato、Fujisawa Shouta、Katayama Hidekazu、Moroder Luis、Ando Setsuko、Okumura Masaki、Inaba Kenji、Hojo Hironobu、Iwaoka Michio
  • Journal Title: Communications Chemistry Volume: 1 Issue: 1 Pages: 26-26
  • DOI: 10.1038/s42004-018-0024-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] クライオ電子顕微鏡単粒子解析が明らかにする小胞体カルシウムポンプSERCA2bの構造機能制御機構 (2020)
  • Author(s): 稲葉 謙次
  • Organizer: 東北大―理研連携シンポジウム
  • Invited
• [Presentation] 亜鉛輸送体によるゴルジ体の亜鉛制御が ERp44機能を調節する (2020)
  • Author(s): 天貝佑太, 山田桃, 小和田俊行, 渡邊朝美, 楢本悟史, 渡部聡, 経塚淳子, 水上進, Roberto Sitia, 稲葉謙次
  • Organizer: 第72回日本細胞生物学会大会
• [Presentation] A new role of cellular zinc for protein quality control in the early secretory pathway (2020)
  • Author(s): Satoshi Watanabe, Yuta Amagai, Sara Sannino, Amiko Miyake, Roberto Sitia, and Kenji Inaba
  • Organizer: 第20回蛋白質科学会年会
• [Presentation] Biochemical characterizations of ER-resident peroxidases, GPx7andGPx8, reveal their distinct oxidative activities (2020)
  • Author(s): Shingo Kanemura, Elza Firdiani Sofia, Naoya Hirai, Masaki Okumura, Hiroshi Kadokura and Kenji Inaba
  • Organizer: 第20回蛋白質科学会年会
• [Presentation] PDIファミリー酵素PDIRの3つのチオレドキシン様ドメインの異なる機能的および化学的特性 (2020)
  • Author(s): 東 晃太、松崎 元紀、渡部 聡、稲葉 謙次
  • Organizer: 第43回日本分子生物学会
• [Presentation] 小胞体における亜鉛結合性シャペロンERp44とクライアントとの複合体の解離機構の生化学的解析 (2020)
  • Author(s): 三宅 杏美子，渡部 聡，天貝 佑太，稲葉 謙次
  • Organizer: 第43回日本分子生物学会
• [Presentation] 小胞体内ジスルフィド結合触媒ネットワークとインスリンフォールディングの品質管理 (2020)
  • Author(s): 奥村正樹、稲葉謙次
  • Organizer: 第43回日本分子生物学会
  • Invited
• [Presentation] Molecular basis for the cooperation of ERp44 and zinc for protein quality control at the ER-Golgi interface (2019)
  • Author(s): Inaba, K.
  • Organizer: The 14th ER & Redox Club Meeting
  • Int'l Joint Research
• [Presentation] Molecular basis for the pH- and zinc-dependent protein quality control at the ER-Golgi interface (2019)
  • Author(s): Inaba, K.
  • Organizer: Structural Biology Seminar at Weissman Institute of Science
  • Invited
• [Presentation] Molecular basis for the pH- and zinc-dependent protein quality control at the ER-Golgi interface (2019)
  • Author(s): Inaba, K.
  • Organizer: Structural Biology Seminar at Technical University of Munich
  • Invited
• [Presentation] Structural basis of zinc-dependent protein quality control in the early secretory pathway (2019)
  • Author(s): Watanabe, S., Amagai, Y., Sannino, S., Yamada, M., Sitia, R. and Inaba, K.
  • Organizer: Cell Biology of Metals, Gordon Research Conference
  • Int'l Joint Research / Invited
• [Presentation] Dynamic assembly/disassembly of Protein Disulfide Isomerase during the catalysis of oxidative protein folding (2019)
  • Author(s): M. Okumura, K. Noi, S. Kanemura, M. Kinoshita, T. Saio, Y. Inoue, T. Hikima, S. Akiyama, T. Ogura, and K. Inaba
  • Organizer: The 14th ER & Redox Club Meeting
  • Int'l Joint Research
• [Presentation] Visualization of zinc in the ER and Golgi (2019)
  • Author(s): Amagai, Y., Yamada, M., Watanabe, T., Kowada, T., Naramoto, S., Watanabe, S., Kyozuka, J., Sitia, R., Mizukami, S. and Inaba, K.
  • Organizer: The 14th ER & Redox Club Meeting
  • Int'l Joint Research
• [Presentation] Mechanisms of translation-coupled oxidative folding (2019)
  • Author(s): C. Hirayama, M. Okumura, K. Machida, K. Noi, T. Ogura, H. Imataka, K. Inaba
  • Organizer: The 14th ER & Redox Club Meeting
  • Int'l Joint Research
• [Presentation] Newly developed thiol-disulfide exchange agent, guanidino-thiol (2019)
  • Author(s): M. Matsusaki, S. Okada, K. Arai, Y. Hidaka, K. Inaba, T. Muraoka, and M. Okumura
  • Organizer: The 14th ER & Redox Club Meeting
  • Int'l Joint Research
• [Presentation] ERp44 acquires Zinc ions at the ER-Golgi intermediate compartment via ZnT7 (2019)
  • Author(s): Amagai, Y., Yamada, M., Watanabe, T., Kowada, T., Naramoto, S., Watanabe, S., Kyozuka, J., Sitia, S., Mizukami, S. and Inaba, K.
  • Organizer: The 6th Meeting of International Society for Zinc Biology
  • Int'l Joint Research
• [Presentation] Mechanisms of disulfide bond formation of newly synthesized polypeptide chains in the endoplasmic reticulum (2019)
  • Author(s): S. Kanemura, M. Okumura, P.J. Robinson, N.J. Bulleid, and K. Inaba
  • Organizer: International Symposium on Disordered Proteins, Protein Folding, and Disease-causing Aggregation
  • Int'l Joint Research
• [Presentation] Coupling effects of thiol and urea-type groups for promotion of oxidative folding (2019)
  • Author(s): M. Matsusaki, S. Okada, K. Inaba, T. Muraoka, and M. Okumura
  • Organizer: International Symposium on Disordered Proteins, Protein Folding, and Disease-causing Aggregation
  • Int'l Joint Research
• [Presentation] Zinc ions regulate ERp44-dependent protein quality control in the early secretory pathway (2019)
  • Author(s): Inaba, K.
  • Organizer: 第19回日本蛋白質科学会年会 第71回日本細胞生物学会大会 合同年次大会
  • Invited
• [Presentation] Novel protein quality control system cooperated by zinc ions and ERp44 in the early secretory pathway (2019)
  • Author(s): Inaba, K.
  • Organizer: 第42回日本分子生物学会年会
  • Invited
• [Presentation] 構造生物学が明らかにする細胞のタンパク質品質管理機構 (2019)
  • Author(s): 稲葉 謙次
  • Organizer: 第１回バイオ単分子研究会
  • Invited
• [Presentation] 亜鉛イオンとシャペロンタンパク質ERp44による新たなタンパク質品質管理機構 (2019)
  • Author(s): 渡部 聡, 天貝佑太, 山田桃, Roberto Sitia, 稲葉謙次
  • Organizer: 第1９回日本蛋白質科学会年会
  • Invited
• [Presentation] 亜鉛が制御する初期分泌経路のタンパク質品質管理機構 (2019)
  • Author(s): 天貝佑太、山田桃、渡邊朝美、小和田俊行、渡部聡、水上進、稲葉謙次
  • Organizer: 第19回日本蛋白質科学会年会
• [Presentation] Mechanistic basis and physiological functions of GPx7 and GPx8, newly identified PDI oxidases in the mammalian endoplasmic reticulum (2019)
  • Author(s): E.F. Sofia, S. Kanemura, H. Kadokura, M. Okumura, and K. Inaba
  • Organizer: 第19回日本蛋白質科学会年会
• [Presentation] PDIファミリー酵素による新生鎖の酸化的フォールディングの触媒機構 (2019)
  • Author(s): 平山千尋、奥村正樹、町田幸大、野井健太郎、小椋光、今高寛晃、稲葉謙次
  • Organizer: 第19回日本蛋白質科学会年会
• [Presentation] 酸化的フォールディングを触媒するPDIの可視化 (2019)
  • Author(s): 奥村正樹、野井健太郎、金村進吾、木下岬、斉尾智英、井上裕一、引間孝明、秋山修志、小椋光、稲葉謙次
  • Organizer: 第19回日本蛋白質科学会年会 第71回日本細胞生物学会大会 合同年次大会
• [Presentation] Understanding the mechanism by PDI family control proteostasis (2019)
  • Author(s): 奥村正樹、稲葉謙次
  • Organizer: 第92回日本生化学大会
  • Invited
• [Presentation] ZnT7 regulates ERp44 through the control of Zn2+ concentrations at the ERGIC/cis-Golgi (2019)
  • Author(s): Yuta Amagai, Momo Yamada, Tomomi Watanabe, Toshiyuki Kowada, Satoshi Naramoto, Satoshi Watanabe, Junko Kyozuka, Roberto Sitia, Shin Mizukami, Kenji Inaba
  • Organizer: 第４２回日本分子生物学会年会
• [Presentation] 新生鎖の立体構造形成を補助するPDIファミリー酵素のジスルフィド結合導入機構 (2019)
  • Author(s): 平山千尋、町田幸大、野井健太郎、奥村正樹、小椋光、今高寛晃、稲葉謙次
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] PDIファミリーメンバーP5の新規構造と機能 (2019)
  • Author(s): 奥村正樹、金村進吾、松崎元紀、木下岬、荒井堅太、平山千尋、天貝佑太、門倉広、秋山修志、稲葉謙次
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] ヒト細胞小胞体機能の低下を鋭敏に検出するレポーターの開発と応用 (2019)
  • Author(s): 八巻聡、河野憲二、稲葉謙次、門倉広
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] ERdj5とBiPの共役による構造異常タンパク質中のジスルフィド結合還元機構 (2019)
  • Author(s): 伊藤 翔悟、井上 道雄、稲葉 謙次
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] 分泌経路局在型カルシウムポンプSPCA1の機能解析 (2019)
  • Author(s): 橋田 丈徳、井上 道雄、稲葉 謙次
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] Structural basis of Sarco/Endoplasmic Reticulum Ca2+-ATPase 2b regulation via transmembrane helix interplay (2019)
  • Author(s): Zhang, Y., Inoue, M., Watanabe, S., Tsutsumi, A., Nishizawa, T., Kikkawa, M., Nagata, K. and Inaba, K.
  • Organizer: 第42回日本分子生物学会年会
• [Presentation] Zinc ions regulate ERp44-dependent protein quality control in the early secretory pathway (2018)
  • Author(s): Inaba, K.
  • Organizer: TAIPEI Tech-IMTRAM joint symposium
  • Int'l Joint Research / Invited
• [Presentation] Zinc ions regulate ERp44-mediated protein quality control in the early secretory pathway (2018)
  • Author(s): Inaba, K.
  • Organizer: International Meeting “Proteins: from the cradle to the grave”
  • Int'l Joint Research / Invited
• [Presentation] Zinc ions have a new physiological role in protein quality control in the early secretory pathway (2018)
  • Author(s): Inaba, K.
  • Organizer: CWRU-Tohoku joint symposium
  • Int'l Joint Research / Invited
• [Presentation] Molecular basis of the ERp44-Zn2+ cooperation for protein quality control in the early secretory pathway (2018)
  • Author(s): Inaba, K.
  • Organizer: 第９１回日本生化学会年会
  • Invited
• [Presentation] Structural basis of pH- and zinc-dependent multiple client recognition by ERp44 (2018)
  • Author(s): Watanabe, S., Amagai, Y., Sitia, R. and Inaba, K.
  • Organizer: 第56回 日本生物物理学会年会
  • Invited
• [Presentation] Structural basis of Sarco/Endoplasmic reticulum Ca2+-ATPase 2b regulation via transmembrane helix interplay (2018)
  • Author(s): 井上 道雄, 作田 菜奈美, 渡部 聡, 田中 良樹, 潮田 亮, 加藤 幸成, 高木 淳一, 塚崎 智也, 永田 和宏 , 稲葉 謙次
  • Organizer: 第56回 日本生物物理学会年会
  • Invited
• [Presentation] Identification of the zinc transporters involved in functional regulation of ERp44 (2018)
  • Author(s): 山田 桃、天貝 佑太、渡部 聡、Sitia Roberto、稲葉 謙次
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] P5による基質認識の分子構造基盤 (2018)
  • Author(s): 奥村 正樹、金村 進吾、松崎元紀、木下岬、荒井 堅太、秋山 修志、稲葉 謙次
  • Organizer: 第18回日本蛋白質科学会年会
• [Presentation] 酸化的フォールディングを触媒するPDIファミリー酵素と新生鎖間のインタープレー (2018)
  • Author(s): 平山千尋、奥村正樹、町田幸大、野井健太郎、小椋光、今高寛晃、稲葉謙次
  • Organizer: 第18回日本蛋白質科学会年会
• [Presentation] 小胞体内腔における新生鎖のジスルフィド結合形成反応の解析 (2018)
  • Author(s): 金村 進吾、奥村 正樹、Neil Bulleid、稲葉 謙次
  • Organizer: 第18回日本蛋白質科学会年会
• [Presentation] Mechanistic basis of GPx7 and GPx8 catalytic cycles for PDI oxidation in concert with hydrogen peroxide (2018)
  • Author(s): Elza F Sofia、金村 進吾、奥村 正樹、稲葉 謙次
  • Organizer: 第18回日本蛋白質科学会年会
• [Presentation] Physiological substrates of PDIp, a pancreas-specific protein disulfide isomerase family member protein (2018)
  • Author(s): Takushi Fujimoto, Orie Nakamura, Michiko Saito, Akio Tsuru, Masaki Matsumoto, Kenji Kohno, Kenji Inaba, Hiroshi Kadokura
  • Organizer: FASEB Science Research Conference Protein Folding in the Cell
  • Int'l Joint Research
• [Presentation] Structural basis of zinc-dependent ERp44 regulation for protein quality control in the early secretory pathway (2018)
  • Author(s): Watanabe, S., Amagai, Y., Sannino, S., Harayama, M., Masui, S., Yamada, M., Sitia, R. and Inaba, K.
  • Organizer: International Meeting “Proteins: from the cradle to the grave
  • Int'l Joint Research
• [Presentation] Zinc ions control the function of ERp44 for protein quality control in the early secretory pathway (2018)
  • Author(s): Yuta Amagai, Satoshi Watanabe, Sara Sannino, Momo Yamada, Tiziana Tempio, Manami Harayama, Roberto Sitia and Kenji Inaba
  • Organizer: International Meeting “Proteins: from the cradle to the grave”
  • Int'l Joint Research
• [Presentation] Elucidation of molecular mechanisms of PDI family enzymes acting on nascent chains in early-stage of translation (2018)
  • Author(s): Chihiro Hirayama, Masaki Okumura, Kodai Machida, Kentaro Noi, Teru Ogura, Hiroaki Imataka and Kenji Inaba
  • Organizer: International Meeting “Proteins: from the cradle to the grave”
  • Int'l Joint Research
• [Presentation] Molecular mechanisms of PDI family enzymes in catalysis of oxidative folding of nascent chains (2018)
  • Author(s): 平山千尋、奥村正樹、町田幸大、野井健太郎、小椋光、今高寛晃、稲葉謙次
  • Organizer: 第４１回日本分子生物学会年会
• [Presentation] 初期分泌経路内のタンパク質品質管理におけるERp44の新たな生理的機能の探索 (2018)
  • Author(s): 天貝佑太、稲葉謙次
  • Organizer: 第４１回日本分子生物学会年会
• [Presentation] Identification of the Zinc Transporter critical for regulation of ERp44, a key protein quality controller in the early secretory pathway (2018)
  • Author(s): Momo Yamada, Yuta Amagai, Satoshi Watanabe, Roberto Sitia, Kenji Inaba
  • Organizer: TAIPEI Tech-IMTRAM joint symposium
  • Int'l Joint Research
• [Presentation] Mechanistic basis of hydrogen peroxide-dependent PDI family oxidation catalyzed by GPx7 and GPx8 (2018)
  • Author(s): Elza Firdiani Sofia, Shing Kanemura, Hiroshi Kadokura, Masaki Okumura, Kenji Inaba
  • Organizer: TAIPEI Tech-IMTRAM joint symposium
  • Int'l Joint Research
• [Book] 実験医学 (2020)
  • Author(s): 天貝佑太、渡部聡、稲葉謙次
  • Total Pages: 8
  • Publisher: 羊土社
• [Book] 日本生化学会誌 (2020)
  • Author(s): 奥村 正樹、稲葉 謙次
  • Total Pages: 6
  • Publisher: 日本生化学会
• [Book] 臨床免疫・アレルギー科 (2020)
  • Author(s): 金村 進吾、松崎 元紀、 前仲 勝実、 稲葉 謙次、 奥村 正樹
  • Total Pages: 8
  • Publisher: 科学評論社
• [Book] 実験医学 (2020)
  • Author(s): 天貝 佑太、渡部 聡、稲葉 謙次
  • Total Pages: 8
  • Publisher: 羊土社
• [Book] 日本生化学会誌 (2020)
  • Author(s): 奥村 正樹、稲葉 謙次
  • Total Pages: 6
  • Publisher: 日本生化学会誌
• [Book] 月刊『細胞』 (2019)
  • Author(s): 奥村 正樹、稲葉 謙次
  • Total Pages: 6
  • Publisher: ニューサイエンス社
• [Book] 放射光利用の手引き「細胞のタンパク質の品質を監査する仕組み」 (2019)
  • Author(s): 渡部 聡、稲葉 謙次
  • Total Pages: 6
  • Publisher: アグネ技術センター
• [Book] 放射光利用の手引き「細胞のタンパク質の立体構造を頑強にする仕組み」 (2019)
  • Author(s): 奥村 正樹、稲葉 謙次
  • Total Pages: 5
  • Publisher: アグネ技術センター
• [Book] Oxidative folding of proteins 2nd Edition (2018)
  • Author(s): Okumura, M., Watanabe, S. and Inaba, K.
  • Total Pages: 25
  • Publisher: Royal Chemical Society
• [Book] アルツハイマー病―発症メカニズムと新規診断法・創薬・治療開発 (2018)
  • Author(s): 奥村 正樹、稲葉 謙次
  • Total Pages: 11
  • Publisher: エヌ・ティー・エス
• [Remarks] 東北大学多元物質科学研究所 稲葉研究室ホームページ
  • URL: http://www2.tagen.tohoku.ac.jp/lab/inaba/html/
• [Remarks] 東北大学多元物質科学研究所生体分子構造研究分野
  • URL: http://www2.tagen.tohoku.ac.jp/lab/inaba/html/
• [Remarks] 東北大学生命科学研究科生体分子構造研究分野 稲葉謙次
  • URL: https://www.lifesci.tohoku.ac.jp/research/teacher/detail.html?id=16689
• [Remarks] 東北大学多元物質科学研究所生体分子構造研究分野publications
  • URL: http://www2.tagen.tohoku.ac.jp/lab/inaba/html/publications1.html
• [Remarks] 東北大学多元物質科学研究所プレスリリース
  • URL: http://www2.tagen.tohoku.ac.jp/lab/news_press/20190213/
• [Patent(Industrial Property Rights)] 液滴及びその製造方法 (2020)
  • Inventor(s): 奥村 正樹、松﨑 元紀、金村 進吾、齋尾 智英、稲葉 謙次
  • Industrial Property Rights Holder: 奥村 正樹、松﨑 元紀、金村 進吾、齋尾 智英、稲葉 謙次
  • Industrial Property Rights Type: 特許
  • Filing Date: 2020
  • Acquisition Date: 2020