| Budget Amount *help |
¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000)
Fiscal Year 2020: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2019: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
Fiscal Year 2018: ¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
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| Outline of Final Research Achievements |
ERp44 is a PDI family member that plays an important role in protein quality control in the early secretory pathway. We recently discovered that zinc ions (Zn2+) regulate structure, affinity for client proteins and intracellular localization of ERp44, leading to its maximal client retention activity. We also developed an organelle-targettable Zn2+ fluorescent probe in collaboration with Prof. Shin Mizukami's group, and succeeded in quantification of labile Zn2+ concentration in the Golgi with the probe. By Zn2+ imaging under the conditions of systematic Zn2+ transporter (ZnT) knockdowns, we revealed that ZnT7 serves as a ZnT that primarily regulates intracellular localization and function of ERp44. Thus, we established molecular and mechanistic basis of the ZnT-mediated Zn2+ homeostasis and ERp44-dependent protein quality control in the early secretory pathway.
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