| Budget Amount *help |
¥44,070,000 (Direct Cost: ¥33,900,000、Indirect Cost: ¥10,170,000)
Fiscal Year 2021: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2020: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2019: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2018: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
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| Outline of Final Research Achievements |
The following results were obtained from studies on translational regulation. (1) Fluorescently labeled pre-miRNA was injected into cultured cells, and miRNA dynamics were analyzed by fluorescence correlation spectroscopy and single molecule imaging. Single miRNA molecules were observed to move along the cytoskeleton. (2) Translation arrest complexes consisting of SecM, mRNA, and ribosomes were bound to cover glass and magnetic beads. When a force was applied by bringing a magnet close to the beads, the translation arrest was released in a load-dependent manner. (3) mRNAs in stress granules were fluorescently labeled and observed by super-resolution microscopy. We found that there were regions of high-density and low-density mRNA in the stress granules.
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