| Budget Amount *help |
¥44,070,000 (Direct Cost: ¥33,900,000、Indirect Cost: ¥10,170,000)
Fiscal Year 2022: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2021: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2020: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2019: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2018: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Outline of Final Research Achievements |
In S. cerevisiae, hydrolysis activity of OST was enhanced in hrd1 mutant, a ubiquitin ligase involved in endoplasmic reticulum-associated degradation (ERAD). After detailed analyses, it was found that formation of OST-mediated free glycans are enhanced under conditions of enhanced accumulation of unfolded proteins, such as ER stress conditions, and free glycans are suggested to play a role on promoting protein folding and/or preventing misfolded proteins from forming protein aggregates. On the other hand, in mammalian cells we established a method for analyzing serum free glycans, for which OST is believed to be involved in the formation, and identified several novel structures. Furthermore, using rat primary hepatocytes it was shown that liver is indeed involved in the secretion of OST-derived serum free glycans.
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