| Budget Amount *help |
¥44,070,000 (Direct Cost: ¥33,900,000、Indirect Cost: ¥10,170,000)
Fiscal Year 2021: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2020: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2019: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2018: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
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| Outline of Final Research Achievements |
In this study, we unraveled the molecular mechanisms driving proteasomal degradation in detail by investigating the molecular network centered on the proteasome and analyzing its changes to certain stressed conditions and cell differentiation. The major findings are as follows: 1) The ubiquitin-selective unfoldase p97-UFD1-NPL4 complex and the shuttle factor RAD23B are critically important for the selection of proteasomal substrates. 2) Under hyperosmotic stress, the proteasomes and ubiquitylated substrates undergo a liquid-liquid phase separation and form proteolytic droplets. 3) Expression level of ECPAS, a proteasome-binding molecule, increases during ES cell differentiation into neurons and is required for cell differentiation.
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