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Elucidation of the molecular mechanisms of the sensing systems and initial responses to osmotic stress in plants

Research Project

Project/Area Number 18H03996
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
Research InstitutionThe University of Tokyo

Principal Investigator

Yamaguchi-Shinozaki Kazuko  東京大学, 大学院農学生命科学研究科(農学部), 特任研究員 (30221295)

Co-Investigator(Kenkyū-buntansha) 溝井 順哉  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (20469753)
城所 聡  東京大学, 大学院農学生命科学研究科(農学部), 特任講師 (70588368)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥44,720,000 (Direct Cost: ¥34,400,000、Indirect Cost: ¥10,320,000)
Fiscal Year 2020: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2019: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2018: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Keywordsシロイヌナズナ / 浸透圧ストレス応答 / シグナル伝達機構 / ストレス感知システム / SnRK2キナーゼ / RAFキナーゼ / オスモセンサー / 植物 / 浸透圧ストレス / リン酸化カスケード
Outline of Final Research Achievements

There are ABA-dependent and ABA-independent signaling pathways in the osmotic stress responses such as drought and high salt in plants. We elucidated that three B4-RAF like protein kinases are interacting factors of the subclass I SnRK2 protein kinases that are activated through an ABA-independent signaling pathway in the early stages of the osmotic stress responses. We also elucidated that these kinases co-localize with the subclass I SnRK2 kinases in the P-bodies and specifically phosphorylate and activate the SnRK2s in response to osmotic stress. Furthermore, transcriptome analyses and growth observation of the multiple mutants showed that these kinases are upstream factors of the subclass I SnRK2s. On the other hand, using similar methods, we also indicated that different RAF like kinases are upstream factors of subclass III SnRK2 protein kinases activated through an ABA-dependent signaling pathway.

Academic Significance and Societal Importance of the Research Achievements

乾燥などの浸透圧ストレスを感知した植物は、遺伝子発現を制御して耐性を獲得するが、そのシグナル伝達系には植物ホルモンのABAを介した経路に加え、ABAを介さない経路が存在する。本研究ではABAが合成される前の初期のストレス応答で重要な機能を果たしている因子であるRAF様キナーゼが同定され、浸透圧ストレスの受容体に迫る重要因子が明らかにされた。得られた研究成果はNature Commun.などのレベルの高い学術誌に掲載された。また、同定された遺伝子は環境ストレス耐性作物開発のために利用できると考えられ、地球環境劣化に対応した食料問題や環境問題の解決に貢献できると期待される。

Report

(3 results)
  • 2021 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (22 results)

All 2020 2019 2018

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (18 results) (of which Int'l Joint Research: 6 results,  Invited: 3 results)

  • [Journal Article] Plant Raf-like kinases regulate the mRNA population upstream of ABA-unresponsive SnRK2 kinases under drought stress2020

    • Author(s)
      Soma Fumiyuki, Takahashi Fuminori, Suzuki Takamasa, Shinozaki Kazuo, Yamaguchi-Shinozaki Kazuko
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 1-12

    • DOI

      10.1038/s41467-020-15239-3

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DREB1A/CBF3 is repressed by transgene-induced DNA methylation in the Arabidopsis ice1-1 mutant2020

    • Author(s)
      Kidokoro Satoshi、Kim June-Sik、Ishikawa Tomona、Suzuki Takamasa、Shinozaki Kazuo、Yamaguchi-Shinozaki Kazuko
    • Journal Title

      The Plant Cell

      Volume: 32 Issue: 4 Pages: 1035-1048

    • DOI

      10.1105/tpc.19.00532

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DNA demethylase ROS1 prevents inheritable DREB1A/CBF3 repression by transgene-induced promoter methylation in the Arabidopsis ice1-1 mutant2020

    • Author(s)
      Kim June-Sik、Kidokoro Satoshi、Shinozaki Kazuo、Yamaguchi-Shinozaki Kazuko
    • Journal Title

      Plant Molecular Biology

      Volume: 104 Issue: 6 Pages: 575-582

    • DOI

      10.1007/s11103-020-01061-4

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis thaliana NGATHA1 transcription factor induces ABA biosynthesis by activating NCED3 gene during dehydration stress.2018

    • Author(s)
      Sato, H., Takasaki, H., Takahashi, F., Suzuki, T., Iuchi, S., Mitsuda, N., Ohme-Takagi, M., Ikeda, M., Seo, M., Yamaguchi-Shinozaki, K., and Shinozaki, K.
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A.

      Volume: 115 Issue: 47

    • DOI

      10.1073/pnas.1811491115

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 乾燥ストレス条件下においてサブクラス I SnRK2 の活性化を担うタンパク質キナーゼの同定2020

    • Author(s)
      相馬史幸,高橋史憲,鈴木孝征,篠崎一雄,篠崎和子
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Identification of protein factors that regulate ABA-unresponsive subclass I SnRK2s in Arabidopsis2019

    • Author(s)
      Fumiyuki Soma, Fuminori Takahashi, Takamasa Suzuki, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
    • Organizer
      the 30th International Conference on Arabidopsis Research
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Signalling pathways and adaptation to drought and temperature stress in plants2019

    • Author(s)
      Kazuko Yamaguchi-Shinozaki
    • Organizer
      Climate Change and plant stresss responses
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] シロイヌナ ズナのストレス応答性転写因子DREB2Aの翻訳後制御に関わるプロテインキナーゼの解析2019

    • Author(s)
      溝井順哉,高橋亮輔,中道範人,木下俊則,篠崎一雄,篠崎和子
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 乾燥ストレス条件下において組織特異的に生合成されるアブシシン酸前駆体およびアブシシン酸の器官間の移動2019

    • Author(s)
      戸高大輔,田川祐眞,最上惇郎,渡邊俊介,瀬尾光範,筒井大貴,川勝弥一,野田口理孝,篠崎和子
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Functional Analysis of 70 kDa Heat Shock Proteins in the Regulation of Heat Stress Responsive Gene Expression in Arabidopsis2019

    • Author(s)
      Asad Jan, Huimei Zhao, Naohiko Ohama, Shinya Koizumi, Kazuya Kusakabe, Junya Mizoi, Satoshi Kidokoro, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 概日時計を介したシロイヌナズナDREB1遺伝子の低温誘導性発現制御2019

    • Author(s)
      城所聡,原口裕基,石川朋奈,戸田智美,篠崎一雄,篠崎和子
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナにおけるサブクラス I SnRK2 の活性を制御するプロテインキナーゼの同定2019

    • Author(s)
      相馬史幸,高橋史憲,鈴木孝征,篠崎一雄,篠崎和子
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] イネにおける転写因子HsfA1 の高温ストレス応答における機能解析2019

    • Author(s)
      野口萌子,大濱直彦,戸高大輔,城所聡,篠崎一雄,篠崎和子
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 葉の形態形成に関わるイネ転写因子OsPIL7 の機能解析2019

    • Author(s)
      橋本貴将,戸高大輔,趙宇,篠崎一雄,篠崎和子
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 浸透圧ストレス時にシロイヌナズナのタンパク質リン酸化酵素SnRK2 の活性を制御する上流因子の同定2019

    • Author(s)
      福井あずさ,相馬史幸,最上惇郎,佐藤花綸,佐藤裕太,高橋史憲,篠崎一雄,篠崎和子
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クチクラ形成を調節する植物の乾燥応答ネットワークの解析2019

    • Author(s)
      浦野薫,圓山恭之進,大島良美,石川寿樹,佐藤繭子,川合真紀,豊岡公徳,篠崎(山口)和子, 篠崎一雄
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 小胞体ストレス応答性転写因子 bZIP17、bZIP28 による根部伸長制御2019

    • Author(s)
      June-Sik Kim,坂本勇貴,高橋史憲,小嶋美紀子,浦野薫,榊原均,松永幸大,篠崎和子,篠崎一雄
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Identification of regulator kinases of mRNA decay under osmotic stress conditions in Arabidopsis,2018

    • Author(s)
      Soma Fumiyuki, Mogami Junro, Sato Yuta, Takahashi Fuminori, Shinozaki Kazuo, Yamaguchi-Shinozaki Kazuko
    • Organizer
      29th International Conference on Arabidopsis Research
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of novel transcription activators of the NCED3 gene that regulates drought stress responses in Arabidopsis Thaliana2018

    • Author(s)
      Hikaru Sato, Hironori Takasaki, Fuminori Takahashi, Satoshi Iuchi, Nobutaka Mitsuda, Masaru Ohme-Takagi, Miho Ikeda, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki
    • Organizer
      29th International Conference on Arabidopsis Research
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Two ER-Anchored Transcription Factors bZIP17 and bZIP28 Jointly Regulate Root Elongation and Cell Growth Genes Expression2018

    • Author(s)
      June-Sik Kim, Yuki Sakamoto, Sachihiro Matsunaga, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki
    • Organizer
      29th International Conference on Arabidopsis Research,
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Different cold-signaling pathways in the responses to rapid and gradual decreases in temperature2018

    • Author(s)
      Kidokoro Satoshi, Takahashi Fuminori, Shinozaki Kazuo, Yamaguchi-Shinozaki Kazuko
    • Organizer
      11th International Plant Cold Hardiness Seminar
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] シロイヌナズナにおける CAMTA転写因子による低温誘導性遺伝子の発現制御2018

    • Author(s)
      城所聡、橋本紫光、高橋史憲、篠崎一雄、篠崎和子
    • Organizer
      第63回低温生物工学会
    • Related Report
      2018 Annual Research Report
    • Invited

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Published: 2018-04-23   Modified: 2024-12-25  

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