| Budget Amount *help |
¥43,940,000 (Direct Cost: ¥33,800,000、Indirect Cost: ¥10,140,000)
Fiscal Year 2021: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2020: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2019: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2018: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
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| Outline of Final Research Achievements |
During ferret cortical development, neural stem cell called apical radial glia (aRG) frequently generate sibling cells, which express Hes1 and bear a short basal process. These cell, termed short radial glia (sRG)l undergo a couple of apical cell divisions with nuclear interkinetic migration just like aRG. Interestingly, the appearance of sRG is stochastic in the lineages. These cells differentiate intermediate progenitor cells expressing Ascl1, and continue several rounds of divisions to produce neurons. While we examine these cell division patterns by time-lapse imaging of slice culture, the consecutive cell cycle number is limited. To overcome this limit, we started in vivo cell lineage analysis using "barclock", which is genome-inserted barcode that accumulates mutation in the presense of genome-editor, hybrid of dCas9 with Cytosine deaminase.
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